Natural resistance in cottonseed to Aspergillus flavus infection has not been explored to date. A green fluorescent protein (GFP) expressing A. flavus strain was used to assess the resistance of seed from 35 cotton varieties from Gossypium arboreum, G. barbadense, and G. hirsutum. Mature cotyledons devoid of seed coat were wounded, inoculated, and assessed for innate resistance to A. flavus infection. Of the initial 35 varieties tested, we observed a range of resistance to infection in representatives of all three species. A subset of 15 representatives was further analyzed. Within this group, G. arboreum cultivar A2 186 and G. hirsutum cultivar SA 1582 were most resistant to fungal infection. The most susceptible cultivar in this group was G. hirsutum SA 1595. The remaining 12 representatives tested in the secondary screen (3 G. arboreum, 3 G. barbadense, and 6 G. hirsutum lines) exhibited intermediate resistance. We did not observe any relationship between species and resistance. Within each species, there was a range of responses to fungal infection. Future studies using this methodology to screen additional diploid and tetraploid cotton lines may enable us to identify naturally resistant germplasm that can be used to develop cotton with enhanced resistance to fungal infection. 相似文献
Efficient water and fertilizer use is of paramount importance both in rain-fed and irrigated rice cultivation systems to tread off between the crop water demand during the dry spell and the fertilizer leaching. This lysimeter study on paddy in a lateritic sandy loam soil of the eastern India, to simulate the water and solute transports using the HYDRUS-1D model, reveals that this model could very well simulate the soil depth-specific variations of water pressure heads and nitrogen (N) concentrations with the efficiency of >86 and 89%, respectively. The change in the level of water ponding depth did not have a significant effect on the time to peak and the temporal variability of N concentration in the bottom soil layer. The lysimeter-scale water balance analysis indicated that the average deep percolation loss and crop water use were 35.01 ± 2.03 and 39.74 ± 1.49% of the total water applied during the crop growth period, respectively. Similarly, the amount of N stored in the plant and lost through soil storage, deep percolation, and other losses (mineralization, denitrification, and gaseous N loss to the atmosphere through plant leaves) were 1.60 ± 0.16, 0.17 ± 0.04, 12.00 ± 0.48, and 86.23 ± 0.41% of the total applied nitrogen, respectively. The simulation results reveal that a constant ponding depth of 3 cm could be maintained in paddy fields to reduce the N leaching loss to 7.5 kgN/ha. 相似文献
Plant landraces have long been recognized as potential gene pools for biotic and abiotic stress-related genes. This research used spring wheat landrace accessions to identify new sources of resistance to the wheat stem sawfly (WSS) (Cephus cinctus Norton), an important insect pest of wheat in the northern Great Plains of North America. Screening efforts targeted 1409 accessions from six geographical areas of the world where other species of grain sawflies are endemic or where a high frequency of accessions possesses the resistance characteristic of solid stems. Resistance was observed in approximately 14% of accessions. Half of the lines displayed both antixenosis and antibiosis types of resistance. Among the resistant accessions, 41% had solid or semi-solid stems. Molecular genetic screening for haplotypes at the solid stem QTL, Qss.msub.3BL, showed that 15% of lines shared the haplotype derived from ‘S-615’, the original donor of the solid stem trait to North American germplasm. Other haplotypes associated with solid stems were also observed. Haplotype diversity was greater in the center of origin of wheat. Evaluation of a representative set of resistant landrace accessions in replicated field trials at four locations over a three year period identified accessions with potential genes for reduced WSS infestation, increased WSS mortality, and increased indirect defense via parasitoids. Exploitation of distinct types of plant defense will expand the genetic diversity for WSS resistance currently present in elite breeding lines. 相似文献
Accelerated erosion removes fertile top soil along with nutrients through runoff and sediments, eventually affecting crop productivity and land degradation. However, scanty information is available on soil and nutrient losses under different crop covers in a vertisol of Central India. Thus, a field experiment was conducted for 4 years (2010–2013) to study the effect of different crop cover combinations on soil and nutrient losses through runoff in a vertisol.
Materials and methods
Very limited information is available on runoff, soil, and nutrient losses under different vegetative covers in a rainfed vertisol. Thus, the hypothesis of the study was to evaluate if different crop cover combinations would have greater impact on reducing soil and nutrient losses compared to control plots in a vertisol.
This experiment consisted of seven treatment combinations of crop covers namely soybean (Glycine max) (CC1), maize (Zea mays) (CC2), pigeon pea (Cajanus cajan) (CC3), soybean (Glycine max)?+?maize (Zea mays) ??1:1 (CC4), soybean (Glycine ma x))?+?pigeon pea (Cajanus cajan) ?2:1 (CC5), maize (Zea mays)?+?pigeon pea (Cajanus cajan) ??1:1 (CC6), and cultivated fallow (CC7). The plot size was 10?×?5 m with 1% slope, and runoff and soil loss were measured using multi-slot devisor. All treatments were arranged in a randomized block design with three replications.
Results and discussion
Results demonstrated that the runoff and soil loss were significantly (p?<?0.05) higher (289 mm and 3.92 Mg ha?1) under cultivated fallow than those in cropped plots. Among various crop covers, sole pigeon pea (CC3) recorded significantly higher runoff and soil loss (257 mm and 3.16 Mg ha?1) followed by that under sole maize (CC2) (235 mm and 2.85 Mg ha?1) and the intercrops were in the order of maize?+?pigeon pea (211 mm and 2.47 Mg ha?1) followed by soybean?+?maize (202 mm and 2.38 Mg ha?1), and soybean?+?pigeon pea (195 mm and 2.15 Mg ha?1). The lowest runoff and soil loss were recorded under soybean sole crop (194 mm and 2.27 Mg ha?1). The data on nutrient losses indicated that the highest losses of soil organic carbon (SOC) (25.83 kg ha?1), total nitrogen (N), phosphorus (P), and potassium (K) (7.76, 0.96, 32.5 kg ha?1) were recorded in cultivated fallow (CC7) as compared to those from sole and intercrop treatments. However, sole soybean and its intercrops recorded the minimum losses of SOC and total N, P, and K, whereas the maximum losses of nutrients were recorded under pigeon pea (CC3). The system productivity in terms of soybean grain equivalent yield (SGEY) was higher (p?<?0.05) from maize?+?pigeon pea (3358 kg ha?1) followed by that for soybean?+?pigeon pea (2191 kg ha?1) as compared to sole soybean. Therefore, maize?+?pigeon pea (1:1) intercropping is the promising option in reducing runoff, soil-nutrient losses, and enhancing crop productivity in the hot sub-humid eco-region.
Conclusions
Study results highlight the need for maintenance of suitable vegetative cover as of great significance to diffusing the erosive energy of heavy rains and also safe guarding the soil resource from degradation by water erosion in vertisols.
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Gorgostane steroids are isolated from marine organisms and consist of 30 carbon atoms with a characteristic cyclopropane moiety. From the pioneering results to the end of 2021, isolation, biosynthesis, and structural elucidation using 13C-NMR will be used. Overall, 75 compounds are categorized into five major groups: gorgost-5-ene, 5,6-epoxygorgostane, 5,6-dihydroxygorgostane, 9,11-secogorgostane, and 23-demethylgorgostane, in addition to miscellaneous gorgostane. The structural diversity, selectivity for marine organisms, and biological effects of gorgostane steroids have generated considerable interest in the field of drug discovery research. 相似文献