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91.
Yang H Jeffrey PD Miller J Kinnucan E Sun Y Thoma NH Zheng N Chen PL Lee WH Pavletich NP 《Science (New York, N.Y.)》2002,297(5588):1837-1848
Mutations in the BRCA2 (breast cancer susceptibility gene 2) tumor suppressor lead to chromosomal instability due to defects in the repair of double-strand DNA breaks (DSBs) by homologous recombination, but BRCA2's role in this process has been unclear. Here, we present the 3.1 angstrom crystal structure of a approximately 90-kilodalton BRCA2 domain bound to DSS1, which reveals three oligonucleotide-binding (OB) folds and a helix-turn-helix (HTH) motif. We also (i) demonstrate that this BRCA2 domain binds single-stranded DNA, (ii) present its 3.5 angstrom structure bound to oligo(dT)9, (iii) provide data that implicate the HTH motif in dsDNA binding, and (iv) show that BRCA2 stimulates RAD51-mediated recombination in vitro. These findings establish that BRCA2 functions directly in homologous recombination and provide a structural and biochemical basis for understanding the loss of recombination-mediated DSB repair in BRCA2-associated cancers. 相似文献
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High-performance liquid chromatography provides direct evidence for substantial removal of naturally occurring specific free amino acids during a single passage of water through the mantle cavity of mussels. This occurs during the few seconds required for passage of the water across the gill, and removal proceeds unabated at ambient substrate concentrations as low as 38 nanomoles per liter. 相似文献
95.
施肥对稻米品质和产量影响的研究 总被引:7,自引:0,他引:7
周瑞庆 《湖南农业大学学报(自然科学版)》1989,(3)
通过1985~1087年的多点田间试验和盆栽试验,探索了施氮量,施氮时期,氮、磷、钾三要素及其组合方式,基肥种类以及稀土微肥对稻米产量和品质的影响,为优质稻合理施肥提供了理论依据。 相似文献
96.
The 12 Virginia barrier islands are divided into three groups on the basis of historical retreat rates and characteristics. The growth and reversal of inlet offsets is a long-term event that results from the operation of known coastal processes. Both geomorphic evidence and wave refraction analyses indicate that shoreline irregularity will diminish with time. There is no basis for the prediction (or expectation) that cape-like features will evolve along this shoreline. 相似文献
97.
Garzione CN Hoke GD Libarkin JC Withers S MacFadden B Eiler J Ghosh P Mulch A 《Science (New York, N.Y.)》2008,320(5881):1304-1307
The surface uplift of mountain belts is generally assumed to reflect progressive shortening and crustal thickening, leading to their gradual rise. Recent studies of the Andes indicate that their elevation remained relatively stable for long periods (tens of millions of years), separated by rapid (1 to 4 million years) changes of 1.5 kilometers or more. Periodic punctuated surface uplift of mountain belts probably reflects the rapid removal of unstable, dense lower lithosphere after long-term thickening of the crust and lithospheric mantle. 相似文献
98.
Meehl GA Washington WM Collins WD Arblaster JM Hu A Buja LE Strand WG Teng H 《Science (New York, N.Y.)》2005,307(5716):1769-1772
Two global coupled climate models show that even if the concentrations of greenhouse gases in the atmosphere had been stabilized in the year 2000, we are already committed to further global warming of about another half degree and an additional 320% sea level rise caused by thermal expansion by the end of the 21st century. Projected weakening of the meridional overturning circulation in the North Atlantic Ocean does not lead to a net cooling in Europe. At any given point in time, even if concentrations are stabilized, there is a commitment to future climate changes that will be greater than those we have already observed. 相似文献
99.
Leland L. Glenna Raymond A. JussaumeJr. Julie C. Dawson 《Agriculture and Human Values》2011,28(2):213-224
Science and technology studies (STS) research challenges the concept of technological determinism by investigating how the
end users of a technology influence that technology’s trajectory. STS critiques of determinism are needed in studies of agricultural
technology. However, we contend that focusing on the agency of end users may mask the role of political-economic factors which
influence technology developments and applications. This paper seeks to mesh STS insights with political-economic perspectives
by accounting for relationships between availability of diverse technologies, variations in political-economic structures,
and farmer interests and characteristics. We present the results of an analysis on the recent development of three wheat varieties:
(a) a wheat variety that was modified genetically to tolerate the herbicide glyphosate, (b) wheat varieties with characteristics
selected to serve specific markets, (c) and emerging research and development of perennial wheat varieties. Using data obtained
through a survey of wheat growers in Washington State, we analyzed whether farmer interest in these three clusters of wheat
varieties was associated with distinct individual characteristics and attitudes and whether those characteristics and attitudes
are consistent with political economic structures. Although our analysis did not allow us to assess the degree of direct influence
that farmers have on the technological development trajectory for these types of wheat, we were able to document variation
in technological alternatives and farmer characteristics related to different political-economic trends. 相似文献
100.
To rigorously test the in vivo cell fate specificity of bone marrow (BM) hematopoietic stem cells (HSCs), we generated chimeric animals by transplantation of a single green fluorescent protein (GFP)-marked HSC into lethally irradiated nontransgenic recipients. Single HSCs robustly reconstituted peripheral blood leukocytes in these animals, but did not contribute appreciably to nonhematopoietic tissues, including brain, kidney, gut, liver, and muscle. Similarly, in GFP+:GFP- parabiotic mice, we found substantial chimerism of hematopoietic but not nonhematopoietic cells. These data indicate that "transdifferentiation" of circulating HSCs and/or their progeny is an extremely rare event, if it occurs at all. 相似文献