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31.
32.
本试验将三七总皂甙(PNS)体外作用于大黄鱼肾细胞,通过活细胞计数和荧光定量PCR检测,初步了解PNS对大黄鱼肾细胞生长及部分免疫相关因子表达的影响。结果显示,40~200μg/mL浓度的PNS对大黄鱼肾细胞生长无明显影响,但浓度达到1 mg/mL以上时,对细胞生长有负面影响;10~1 000μg/mL浓度的PNS对大黄鱼肾细胞Toll样受体5mRNA的表达有一定的上调作用,但对Toll样受体3的表达有一定的下调作用;PNS浓度为10~100μg/mL时对大黄鱼肾细胞碱性磷酸酶的表达无明显影响,但浓度达到1 000μg/mL时对该因子的表达有下调作用;PNS浓度为10μg/mL时对大黄鱼肾细胞溶菌酶的表达无明显影响,但浓度达到100μg/mL以上时对该因子的表达具有一定的下调作用。 相似文献
33.
OBJECTIVE: To evaluate the effects on intraocular pressure (IOP), pupillary diameter (PD), blepharospasm score, conjunctival injection score, and aqueous humor flare score when either 0.03% bimatoprost solution is applied once daily or 0.15% unoprostone isopropyl solution is applied twice daily topically to the eyes of normal cats. MATERIALS AND METHODS: The aforementioned parameters were evaluated daily in each of 12 cats throughout the entirety of the study. During an initial 10-day treatment phase a single eye of six of the cats was treated with 0.03% bimatoprost solution while a single eye of the remaining six cats was treated with buffered saline solution (BSS) once daily. During a second 10-day treatment phase a single eye of six of the cats was treated with 0.15% unoprostone isopropyl solution while a single eye of the remaining six cats was treated with BSS twice daily. Contralateral eyes of all cats remained untreated at all time points. RESULTS: Blepharospasm score, conjunctival injection score, and aqueous humor flare score never rose from a value of 0, for any eye of any cat during the study. The mean +/- SD of IOP for eyes treated with 0.03% bimatoprost solution and BSS were 16.55 +/- 3.06 mmHg and 18.02 +/- 3.52 mmHg, respectively. The mean +/- of PD for eyes treated with 0.03% bimatoprost solution and BSS were 5.7 +/- 1.57 mm and 6.39 +/- 1.78 mm, respectively. The mean +/- SD of IOP for eyes treated with 0.15% unoprostone isopropyl solution and BSS were 15.7 +/- 2.91 mmHg and 17.2 +/- 2.9 mmHg, respectively. The mean +/- SD of PD for eyes treated with 0.15% unoprostone isopropyl solution and BSS were 5.8 +/- 1.43 mm and 6.9 +/- 1.37 mm, respectively. There was no significant difference (P > or = 0.05) in IOP or PD between eyes treated with 0.03% bimatoprost solution vs. eyes treated with BSS. Similarly, there was no significant difference (P > or = 0.05) in IOP or PD between eyes treated with 0.15% unoprostone isopropyl solution vs. eyes treated with BSS. CONCLUSION: Neither once daily topical administration of 0.03% bimatoprost solution nor twice daily topical administration of 0.15% unoprostone isopropyl solution significantly affect the IOP of normal cats. Both 0.03% bimatoprost solution and 0.15% unoprostone isopropyl solution induced no significant ocular side effects in normal cats when dosed over a 10-day treatment period. 相似文献
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A 9-month-old, castrated male domestic shorthair cat with urinary incontinence was referred for surgical correction of an ectopic ureter. Excretory urography revealed hydronephrosis of the right kidney, right hydroureter, and ureterocele. A partial ureterocelectomy and neoureterocystostomy were performed. This report describes the surgical modification of the ureterocele and ectopic ureter to correct ipsilateral hydronephrosis, hydroureter, and urinary incontinence. 相似文献
36.
为建立一种灵敏、准确、快速的山羊痘病毒(GTPV)和小反刍兽疫病毒(PPRV)双重PCR检测方法,根据GenBank上登录的GTPV、PPRV两种病毒的基因序列设计检测引物,在常规PCR基础上,对反应条件及反应体系进行优化,建立了这两种病毒的双重PCR检测方法,并进行了特异性、敏感性检验。结果显示:仅GTPV和PPRV核酸样品扩增出与预期目标相符的目的片段,而其他病原对照均未出现目的条带;GTPV的最低检出量为0.283 ng/μL,PPRV的最低检出量为6.459 ng/μL。结果表明,本研究建立的双重PCR检测方法可一次性同时完成PPRV、GTPV两种病原的检测,且具有较高的特异性和敏感性。本方法的建立为山羊痘和小反刍兽疫的临床诊断、流行病学研究、疫情监测提供了技术支持。 相似文献
37.
昆明草坪草混播配方优化组合模式探讨 总被引:1,自引:0,他引:1
在昆明独特的气候条件下,通过对运动场草坪、观赏草坪和护坡草坪三类草坪混播配方的调查研究,探讨出了适应云南地区种植的9种优化组合。运动场草坪:草地早熟禾(Poapratensis L.)+高羊茅(Festucaarundinacea Schreb.)+多年生黑麦草(Lolium perenne L.),草地早熟禾+高羊茅,草地早熟禾+狗牙根(Cynodon dactylon(L.)Pers、)+多年生黑麦草;观赏草坪:草地早熟禾+匍匐翦股颖(Agrostisstolonifera L.),草地早熟禾+紫羊茅(Festuca rubraL.),草地早熟禾(优异)+草地早熟禾(蓝月)+草地早熟禾(新哥来得)+草地早熟禾(肯塔基);护坡草坪:高羊茅+多年生黑麦草+狗牙根,高羊茅+多年生黑麦草+狗牙根+白三叶(Trifolium repens L.),高羊茅+多年生黑麦草+狗牙根+白三叶+紫花苜蓿(Medicago sativa L.)。 相似文献
38.
酪蛋白钙肽的研究进展及其在乳品中的应用 总被引:1,自引:0,他引:1
酪蛋白钙肽是含有磷酸丝氨酸残基的多肽,现已证明其具有重要的生理功能。本文介绍酪蛋白钙肽的功能、制备工艺、检测方法,以及与酪蛋白磷酸肽区别,并简述其在乳品中的应用。 相似文献
39.
准确理解中度干扰假说和冗余结构理论,并将两者有机结合,有助于草地质量的调控。关于这两种生态学理论所取得的相关研究成果,对于认识和理解草地生态系统的结构、功能、演替以及生产潜力具有积极作用,这也使其逐步成为指导草地畜牧业生产实践十分重要的科学依据。阐述了草地质量及其具体表示指标,以及中度干扰假说和冗余结构理论与草地质量的关系,并论述了中度干扰假说和冗余结构理论在封育、中度放牧、毒害草防治等管理措施条件下草地质量变化的生态学过程中的作用。 相似文献
40.
Dietary supplementation with Acanthopanax senticosus extract enhances gut health in weanling piglets
Jun Fang F.Y. Yan X.F. Kong Z. Ruan Z.Q. Liu R.L. Huang T.J. Li M.M. Geng F. Yang Y.Z. Zhang Peng Li Joshua Gong G.Y. Wu M.Z. Fan Y.L. Liu Y.Q Hou Y.L. Yin 《Livestock Science》2009,123(2-3):268-275
The present study was conducted to investigate the effects of Acanthopanax senticosus extract (ASE) as a dietary additive on gut health in weanling piglets by examining diarrhea frequency, intestinal microbiota and morphology. A total of 96 Duroc× (Landrace × Yorkshire) piglets weaned at 21 days of age with an average initial body weight (BW) of 5.6 ± 0.4 kg were randomly assigned to 3 treatment groups with 4 duplicates of 8 piglets each. The piglets were fed basal diet to which had been added 0 or 1 g/kg of ASE, or 0.7 g/kg antibiotics, respectively. Fecal consistence was monitored twice daily and the frequency of diarrhea was calculated. On day 21 after the initiation of supplementation, 8 piglets were randomly selected from each treatment group (2 piglets per pen) and slaughtered. The jejunum, ileum, colon and cecum were then excised and fixed in 10% neutral formalin solution to determine villus height and crypt depth, after their contents were collected to determine microbiota. The results showed that dietary supplementation with ASE increased (P < 0.05) the density of bacterial populations that co-migrated with Lactobacillus amylovorus, Lactobacillus salivarius, Bacillus subtilis, and Clostridium lituseburens, but decreased (P < 0.05) those co-migrating with Staphylococcus aureus, Salmonella typhimurium, Ruminococcus forques, and E. coli O157:H7 in the PCR-DGGE profiling analysis when compared with the control group. The villus height of the duodenum, jejunum and ileum increased (P < 0.05) by 14.8, 13.7 and 10.0%, while the crypt depth decreased (P < 0.05) by 17.9, 9.1 and 12.1%, respectively, in response to dietary ASE supplementation. Additionally dietary supplementation with ASE or an antibiotic decreased (P < 0.05) the frequency of diarrhea by 55.6 and 52.2%, respectively, compared with the control group. In conclusion, these findings suggest that dietary supplementation with ASE could regulate the microbiota composition and maintain a normal morphology of gut mucosa in weanling piglets, thereby decreasing diarrhea that resulted from weaning stress. 相似文献