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991.
992.
993.
Breeding Restrictions Decrease the Prevalence of Myxomatous Mitral Valve Disease in Cavalier King Charles Spaniels over an 8‐ to 10‐Year Period 下载免费PDF全文
994.
Diagnostic Value of Right Pulmonary Artery Distensibility Index in Dogs with Pulmonary Hypertension: Comparison with Doppler Echocardiographic Estimates of Pulmonary Arterial Pressure 下载免费PDF全文
995.
Retrospective Evaluation of Horses Diagnosed with Neuroborreliosis on Postmortem Examination: 16 Cases (2004–2015) 下载免费PDF全文
996.
997.
L. S. Gay D. S. Kronfeld PhD DSc MVSc A. Grimsley-Cook DVM MS J. J. Dascanio DVM A. O. Ordakowski-Burk PhD R. K. Splan PhD E. A. Dunnington PhD D. J. Sklan PhD 《Journal of Equine Veterinary Science》2004,24(3):115-120
Fat-soluble vitamins transfer poorly across the diffuse epitheliochorial placenta of the mare, so the neonatal foal is dependent on its supply via colostrum. Concentrations of retinol (RT), β-carotene (BC), and β-tocopherol (AT) were assayed in samples of plasma, colostrum and milk from nine mares of mixed light breeding and their foals at parturition and days 1, 2, and 4 postpartum. Samples were analyzed simultaneously for RT, BC, and AT using a new, less time-consuming reverse phase high-pressure liquid chromatography (HPLC) method. Feeds were analyzed, and mean daily intakes calculated. Mare plasma RT increased from day 1 to day 4 (P = .033), and plasma AT declined linearly from day 0 to day 4 (P = .006). Colostrum concentrations of all vitamins increased from parturition to day 1, and then declined rapidly over the 4 days. Foal plasma BC increased from parturition to day 4 (P = .080), and plasma AT increased from parturition to day 2 (P < .001), and 4 (P = .060). These observations suggest that three times the current recommendation of vitamin A is sufficient for pregnant mares, in keeping with previous studies of growth, pregnancy, and lactation. In contrast, the linear decrease in mare plasma AT suggests that 1.2 times the current recommendation of vitamin E may be less than optimal for pregnant mares.
Introduction
The diffuse epitheliochorial placenta of the mare does not allow fat-soluble vitamins to cross with ease to the fetus,[1] so their status in the neonatal foal is dependent on colostrum. This first milk contains these vitamins, which are necessary for many functions.Vitamin A is required for vision, reproduction, growth, development, and maintenance of epithelial cells, and osteoclast activity in bone formation. In addition, vitamin A deficiencies reduce resistance to disease, can cause neurological degeneration, and congenital defects.[2] Vitamin E, an antioxidant, prevents lipid peroxidation and cell membrane damage. Its deficiency in horses has been associated with a form of muscular dystrophy and impaired immune function. [3]Studies on the mare and foal have described blood concentrations of one or two of these vitamins,[4, 5 and 6] but only one involved all three. [7] In that study, vitamin concentrations were assayed in mare blood and milk, but only once in foal blood at parturition, so data during the periparturant period is incomplete.The objectives of this enumerative study were to determine concentrations of RT, BC, AT in plasma and colostrum, to evaluate colostral transfer of these vitamins, to assess the adequacy of vitamin intakes of mares, and to develop a time-saving single-run HPLC method.Materials and methods
Animals
Nine healthy aged multiparous mares of mixed light breeding (13.6 ± 1.5 years, 592 ± 20 kg body mass) and their foals were sampled. The institutional animal care and use committee approved the protocol. Mares were kept on meager late-winter mixed grass pasture, with free access to round bale orchard and fescue grass hay, water, and trace mineral/vitamin salt. They were fed 2 kg of concentrate twice daily at 7:00 and 3:00 . The diet met or exceeded current recommendations for pregnant mares at 11 months gestation for energy.[8] Samples of hay and concentrate were submitted for partial proximate analysis to the Virginia Tech Forage Testing Laboratory where standard AOAC [9] methods are used ( Table 1). Samples of feeds were also taken for analysis of RT, BC, and AT ( Table 2). 相似文献998.
Effects of dietary lysine and energy density on performance and carcass characteristics of finishing pigs fed ractopamine 总被引:2,自引:0,他引:2
Apple JK Maxwell CV Brown DC Friesen KG Musser RE Johnson ZB Armstrong TA 《Journal of animal science》2004,82(11):3277-3287
Two hundred sixteen crossbred barrows and gilts (84.3 kg BW) were used to test the effects of dietary energy density and lysine:energy ratio (Lys:ME) on the performance, carcass characteristics, and pork quality of finishing pigs fed 10 ppm ractopamine. Pigs were blocked by BW and gender, allotted to 36 pens (six pigs per pen), and pens were assigned randomly within blocks to dietary treatments (as-fed basis) arranged in a 2 x 3 factorial design, with two levels of energy (3.30 or 3.48 Mcal/kg) and three Lys:ME (1.7, 2.4, or 3.1 g lysine/Mcal) levels. Pigs were fed experimental diets for 28 d, and weights and feed disappearance were recorded weekly to calculate ADG, ADFI, and G:F. Upon completion of the feeding trial, pigs were slaughtered and carcass data were collected before fabrication. During carcass fabrication, hams were analyzed for lean composition using a ham electrical conductivity (TOBEC) unit, and loins were collected, vacuum-packaged, and boxed for pork quality data collection. Energy density had no (P > 0.22) effect on ADG or ADFI across the entire 28-d feeding trial; however, pigs fed 3.48 Mcal of ME were more (P < 0.02) efficient than pigs fed 3.30 Mcal of ME. In addition, ADG and G:F increased linearly (P < 0.01) as Lys:ME increased from 1.7 to 3.1 g/Mcal. Carcasses of pigs fed 3.48 Mcal of ME were fatter at the last lumbar vertebrae (P < 0.08) and 10th rib (P < 0.04), resulting in a lower (P < 0.03) predicted fat-free lean yield (FFLY). Conversely, 10th-rib fat thickness decreased linearly (P = 0.02), and LM depth (P < 0.01) and area (P < 0.01) increased linearly, with increasing Lys:ME. Moreover, FFLY (P < 0.01) and actual ham lean yield (P < 0.01) increased as Lys:ME increased in the diet. Dietary energy density had no (P > 0.19) effect on pork quality, and Lys:ME did not (P > 0.20) affect muscle pH, drip loss, color, and firmness scores. Marbling scores, as well as LM lipid content, decreased linearly (P < 0.01) as Lys:ME increased from 1.7 to 3.1 g/Mcal. There was a linear (P < 0.01) increase in shear force of cooked LM chops as Lys:ME increased in the finishing diet. Results indicate that 3.30 Mcal of ME/kg (as-fed basis) is sufficient for optimal performance and carcass leanness in pigs fed ractopamine. The Lys:ME for optimal performance and carcass composition seems higher than that currently used in the swine industry; however, feeding very high Lys:ME (> 3.0 g/Mcal, as-fed basis) to ractopamine-fed pigs may result in decreased marbling and cooked pork tenderness. 相似文献
999.
Lawrence KR Goodband RD Tokach MD Dritz SS Nelssen JL Derouchey JM 《Journal of animal science》2004,82(12):3635-3645
Five experiments were conducted to determine the effects of different wheat gluten (WG) sources (Source 1 = enzymatically hydrolyzed, Source 2 = nonmodified ring-dried, Source 3 = spray-dried, and Source 4 = flash-dried) on growth performance of nursery pigs compared with soybean meal (SBM), spray-dried animal plasma (SDAP), or other specialty protein sources. In Exp. 1, pigs (n = 220, initially 6.1 +/- 2.5 kg) were fed a control diet containing (as-fed basis) 6% SDAP or WG Source 1 or 2. The WG and l-lysine*HCl replaced 50 or 100% of the SDAP. From d 0 to 21, increasing WG (either source) decreased ADG and ADFI (linear, P < 0.01), but improved (linear, P < 0.02) G:F. In Exp. 2, pigs (n = 252, initially 6.2 +/- 3.0 kg) were fed a negative control diet containing no SDAP or WG, diets containing (as-fed basis) 9% WG Source 1 or 5% SDAP, or combinations of WG and SDAP where WG and l-lysine*HCl replaced 25, 50, or 75% of SDAP. From d 0 to 14, pigs fed increasing WG had decreased ADG (linear, P < 0.05). In Exp. 3, pigs (n = 240, initially 7.0 +/- 2.5 kg) were fed a negative control diet, a diet containing (as-fed basis) either 3, 6, 9, or 12% WG Source 3, or a positive control diet containing 5% SDAP. The diets containing 9% WG and 5% SDAP had the same amount of SBM. From d 0 to 7, pigs fed 5% SDAP had greater (P < 0.04) ADG than pigs fed the diet containing 9% WG. From d 0 to 14, increasing WG had no effect on ADG, ADFI, or G:F. In Exp. 4, pigs (n = 200, initially 6.0 +/- 2.4 kg) were fed a negative control diet, the control diet with (as-fed basis) 4.5 or 9.0% WG Source 1, or the control diet with 2.5 or 5.0% SDAP. Diets containing WG and SDAP had similar SBM levels. From d 0 to 7 and 0 to 14, increasing SDAP tended to improve (linear, P < 0.06) ADG, but increasing WG had no effect. In Exp. 5, 170 barrows and gilts (initially 7.5 +/- 2.8 kg) were used to determine the effects of WG Source 1 and 4 compared with select Menhaden fish meal or spray-dried blood cells and a negative control diet (SBM) on the growth performance of nursery pigs from d 5 to 26 postweaning (d 0 to 21 of experiment). No differences were found in ADG or G:F, but pigs fed the diet containing (as-fed basis) 2.5% spray-dried blood cells had greater ADFI than pigs fed the negative control from d 0 to 21. Wheat gluten source had no effect on ADG, ADFI, or G:F. The results of these studies suggest that increasing WG in diets fed immediately after weaning did not improve growth performance relative to SBM or SDAP. 相似文献
1000.
Mylonakis ME Koutinas AF Breitschwerdt EB Hegarty BC Billinis CD Leontides LS Kontos VS 《Journal of the American Animal Hospital Association》2004,40(3):174-184
Nineteen dogs from Greece with chronic ehrlichiosis were studied. The dogs exhibited bicytopenia or pancytopenia, bone marrow hypoplasia, seroreactivity to Ehrlichia canis (E. canis) antigens, and had no history of drug or radiation exposure. Anorexia, depression, severe bleeding tendencies, hypoalbuminemia, and increased serum alanine aminotransferase activity were also hallmarks of the disease. All these animals eventually died, irrespective of the treatment applied. Some dogs were also serologically positive for Rickettsia conorii, Leishmania infantum (L. infantum), and Bartonella vinsonii subspp. berkhoffii. Polymerase chain reaction testing of bone marrow samples revealed E. canis, Anaplasma phagocytophilia, Anaplasma platys, and L. infantum in some dogs. Concurrent infections did not appear to substantially influence the clinical course and final outcome of the chronic canine ehrlichiosis. 相似文献