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71.
A high-performance liquid chromatography (HPLC) method was developed to quantitatively analyze oleocanthal in extra virgin olive oils. Oleocanthal, a deacetoxy ligstroside aglycone, is known to be responsible for the back of the throat irritation of olive oils and to have probated antiinflamatory activity. Oleocanthal was isolated from small amounts of olive oil sample (1 g) by liquid-liquid extraction. Hexane-acetonitrile was found to be the best solvent system to extract oleocanthal from the oil matrix. The solvent extract was analyzed by reversed-phase HPLC with UV detection at 278 nm. Chromatogaphic separation of oleocanthal from other extracted compounds and of the two geometric isomers of oleocanthal was achieved by an elution gradient with acetonitrile and water. Both the external standard calibration curve and the internal standard calibration curve were established, and quantitation using both calibration curves gave essentially the same result. The reproducibility (RSD = 4.7%), recovery (> 95%), and limit of quantitation (< 1 microg/g) were also determined. Concentrations of oleacanthal in 10 selected throat-burning extra virgin olive oils were determined using the method (ranged from 22 to 190 microg/g) with external standard calibration. 相似文献
72.
Judi Krzyzanowski Ian G. McKendry John L. Innes 《Water, air, and soil pollution》2006,173(1-4):273-287
During the summers of 2001 and 2002, hourly average ozone concentrations were measured at three sites of differing elevation (188, 588, and 1221 m.a.s.l.) on the forested south-facing slopes of the Lower Fraser Valley (LFV), British Columbia. Sites experienced ozone concentrations ranging from 0 to 88 ppb in 2001, and 0 to 96 ppb in 2002. Daily patterns were in agreement with previous studies showing morning increases and late afternoon peaks. Reduced diurnal variation increased the exposure of higher-elevation forested sites. An upper-level ridge coinciding with a thermal coastal trough caused above-average ozone concentrations, and the ‘maximum acceptable’ 1-hour National Ambient Air Quality Objective (AQO) of 82 ppb to be exceeded. Maximum ozone concentrations and AQO exceedance frequency both increased with distance eastward in the valley. A preliminary survey of ozone-like injury symptoms on native shrubs suggested that the elevated ozone levels occurring in the LFV may cause injury to forest plants. 相似文献
73.
Li L Tsao R Yang R Kramer JK Hernandez M 《Journal of agricultural and food chemistry》2007,55(4):1164-1169
The fatty acid and tocopherol compositions of three heartnut (Juglans ailanthifolia var. cordiformis) varieties (Imshu, Campbell CW1, and Campbell CW3) were examined and compared with those of two Persian walnut (Juglans regia L.) varieties (Combe and Lake). The major fatty acids found in heartnuts and walnuts were identified by gas chromatography as linoleic (18:2n-6), alpha-linolenic (18:3n-3), oleic (18:1n-9), palmitic (16:0), and stearic acid (18:0). Polyunsaturated fatty acids were the main group of fatty acids found in both heartnut and walnut, ranging from 73.07 to 80.98%, and were significantly higher in heartnut than in Persian walnuts (P < 0.001). In addition, heartnuts had significantly higher levels of 18:2n-6 and lower levels of 18:3n-3 compared to the Persian walnuts. gamma-Tocopherol was the main tocopherol homologue present in both types of nuts, followed by delta- and alpha-tocopherol. The highest concentration of gamma-tocopherol was found in Combe Persian walnut at 267.87 mug/g, followed by Lake Persian walnut and Imshu, Campbell CW1, and CW3 heartnut at 205.45, 187.33, 161.84, and 126.46 mug/g, respectively. Tocopherols, particularly the gamma-tocopherol, were found to contribute the most to the strong total antioxidant activities of both walnut and heartnut oils using either the free radical 2,2-diphenyl-1-picrylhydrazyl assay or the photochemiluminescence method. 相似文献
74.
Assessment of EST- and genomic microsatellite markers for variety discrimination and genetic diversity studies in wheat 总被引:22,自引:0,他引:22
Fiona Leigh Vince Lea John Law Petra Wolters Wayne Powell Paolo Donini 《Euphytica》2003,133(3):359-366
It is likely that in the near future sequence information from sequencing programmes and EST libraries will generate an abundance
of genic microsatellite markers. This study is focused on the assessment of their likely impact and performance vis-à-vis
their genomic counterparts. Microsatellites from two sources were used to assess the genetic diversity in 56 old and new varieties
of bread wheat on the UK Recommended List. A set of 12 microsatellite markers generated from genomic libraries and 20 expressed
sequence tag (EST)-derived microsatellites were used in the study, and the performance of both marker sets assessed. The EST-derived
or genic microsatellites delivered fingerprints of superior quality, amplifying clear products with few stutter bands. Diversity
levels as revealed bygenic microsatellites are similar to the few published results. The PIC values for the genic markers
were generally lower than those calculated for the genomic microsatellites, though advantages of both marker classes for variety
identification applications are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
75.
Historical Trace Metal Accumulation in the Sediments of an Urbanized Region of the Lake Champlain Watershed, Burlington, Vermont 总被引:3,自引:0,他引:3
Ellen L. Mecray John W. King Peter G. Appleby Allen S. Hunt 《Water, air, and soil pollution》2001,125(1):201-230
This study documents the history ofpollution inputs in the Burlington region of LakeChamplain, Vermont using measurements of anthropogenicmetals (Cu, Zn, Cr, Pb, Cd, and Ag) in four age-datedsediment cores. Sediments record a history ofcontamination in a region and can be used to assessthe changing threat to biota over time and to evaluatethe effectiveness of discharge regulations onanthropogenic inputs.Grain size, magnetic susceptibility, radiometricdating and pollen stratigraphy were combined withtrace metal data to provide an assessment of thehistory of contamination over the last 350 yr inthe Burlington region of Lake Champlain. Magneticsusceptibility was initially used to identify land-usehistory for each site because it is a proxy indicatorof soil erosion. Historical trends in metal inputs inthe Burlington region from the seventeenth through thetwentieth centuries are reflected in downcorevariations in metal concentrations and accumulationrates. Metal concentrations increase above backgroundvalues in the early to mid nineteenth century. Themetal input rate to the sediments increases around1920 and maximum concentrations and accumulation ratesare observed in the late 1960s. Decreases inconcentration and accumulation rate between 1970 andthe present are observed for most metals. Theobserved trends are primarily a function of variationsin anthropogenic inputs and not variations in sedimentgrain size. Grain size data were used to removetexture variations from the metal profiles and resultsshow trends in the anthropogenic metal signals remain. Radiometric dating and pollen stratigraphy providewell-constrained dates for the sediments therebyallowing the metal profiles to be interpreted in termsof land-use history. 相似文献
76.
Manufactured soil for landscaping purposes was produced by composting for 6 weeks (1) municipal green waste alone, (2) green
waste amended with 25% v/v poultry manure, or (3) green waste immersed in, and then removed from, a mixture of liquid grease trap waste/septage. Composting
temperatures increased most rapidly and reached highest values (78oC) in the grease trap/septage-amended green waste. In comparison
with green waste alone, addition of poultry manure prolonged the period of elevated temperatures and increased the maximum
temperature attained from 52oC to 61oC. Following composting, each of the materials was split into (1) 100% compost, (2) 80%
compost plus 20% v/v soil, and (3) 70% compost plus 20% soil plus 10% coal fly ash. Addition of poultry manure or grease trap/septage to green
waste prior to composting increased bulk density and reduced total porosity of the composted product. Addition of soil, or
soil and ash, to composts increased bulk density, reduced total porosity, decreased percentage macropores, and increased percentage
mesopores and available water-holding capacity. Bicarbonate-extractable P, exchangeable NH4+ and NO3−, electrical conductivity (EC), soluble C, soluble C as a percentage of organic C, basal respiration, and metabolic quotient
were all markedly greater in the grease trap/septage-amended than poultry manure-amended or green waste alone treatments.
Values for extractable P and EC were considered large enough to be damaging to plant growth and germination index (GI) of
watercress was less than 60% for all grease trap/septage composts. Extractable P and EC were also high, and GI was <100%,
in the green waste alone and poultry manure-amended green waste alone treatments. Addition of soil or soil and ash to these
composts resulted in GI values >100%. 相似文献
77.
Snyder Marcía N. Schumaker Nathan H. Ebersole Joseph L. Dunham Jason B. Comeleo Randy L. Keefer Matthew L. Leinenbach Peter Brookes Allen Cope Ben Wu Jennifer Palmer John Keenan Druscilla 《Landscape Ecology》2019,34(5):1197-1197
Landscape Ecology - In the original publication of the article, the third author name has been misspelt. The correct name is given in this Correction. The original version of this article was revised. 相似文献
78.
79.
Shamuyarira Kwame Wilson Shimelis Hussein Tapera Terence Tsilo Toi John 《Journal of Crop Science and Biotechnology》2019,22(2):169-176
Journal of Crop Science and Biotechnology - Genetic variation and heritability estimates in early generations are important in identifying superior families that can be targeted for genetic... 相似文献
80.
John B. Graham Joan I. Nassauer William S. Currie Herbert Ssegane M. Cristina Negri 《Landscape Ecology》2017,32(5):1023-1037