云水资源的生态和环境功探讨及其应用

为探讨云水资源优化配置的理论依据,文章以二元水循环规律为理论基础,通过对空中水、地表水、土壤水和地下水四水转化的关系和着陆后云水资源存在形式的分析,提出生态和环境功的概念并给出云水资源生态和环境功的计算模型,来描述云水资源的生态环境利用效率。以研究区为例进行应用研究,在雨季末期人工降水20mm时,用此模型计算了研究区三个子区域生态和环境功。应用得到两点主要结论:1)云水资源生态和环境功以地表径流水量生态和环境功为主。2)受水区得到云水资源转化的土壤水和地下水水量受到其初始土壤含水率和初始地下水位限制。应用结果表明云水资源生态和环境功模型函数可以作为云水资源优化配置的理论依据。     

荒漠刺叶墙藓DNA的提取及PCR扩增反应条件

采用改进CTAB法、NaOH法和直接PCR法(direct PCR amplification)提取藓类生物结皮中刺叶墙藓(Tortula desertorumBroth.)基因组DNA,比较其分离效果以及ISSR扩增效果。结果表明:改进CTAB法提取的基因组DNA质量好、纯度高,ISSR扩增反应效果好。直接PCR法一旦体系建立,则是最简易快速、经济高效的模板制备方法,适于植株矮小、生物量很小的荒漠藓类植物个体模板DNA的制备。利用改进CTAB法获得的基因组DNA作为模板,对ISSR反应组分浓度及反应程序中的一些重要参数进行摸索和优化实验,建立了刺叶墙藓最优ISSR反应体系。反应总体积为20μL,各反应组分终浓度为:2μL10×Buffer,2 mM Mg2 ,0.1 mM dNTPs,0.2μM引物,10~40 ng模板DNA,1U Taq酶。扩增程序为:94℃4 min,1个循环;94℃45 s,退火温度(Tm)45 s,72℃1 min,35个循环;72℃7 min,1个循环;4℃保存。刺叶墙藓是组成古尔班通古特沙漠藓类生物结皮的优势种,研究结果为进一步开展刺叶墙藓遗传多样性的研究奠定了基础。     

芋优异种质资源鉴定评价研究

对农业行业标准《农作物种质资源鉴定评价规范芋》编制的技术路线进行了分析研究,以期为芋优异种质资源的鉴定评价提供参考。     

虎皮病的防治研究概况

虎皮病的防治主要包括化学和非化学防治。化学防治的方法很多,目前最有效的是使用二苯胺,但是人类出于对自身健康的考虑,许多消费者已开始不愿接受化学处理的果实,非化学防治成为人们研究的热点。非化学防治主要包括气调贮藏、采后热处理和虎皮病预测。     

用雌性系配制的甜瓜新品种‘寿研1号’及其指纹图谱

 厚皮甜瓜‘寿研1号’是以回交转育的雌性系‘白9298gy’为母本, 以‘玉9635’自交系 为父本配制的一代杂交种。中熟, 果实发育期33～36 d。果实高圆形, 单瓜质量115～210 kg, 果皮光滑洁白, 果肉细腻, 白色略带浅绿, 糖度16.5%左右。易坐果, 耐低温, 抗病性强, 适于保护地栽培。用8对引物构建了其重复概率为1 /291 600的SSR分子标记的指纹图谱。     

Effects of baicalin on CA46 cell xenografts in nude mice

AIM: To study the effects of baicalin on CA46 cell xenografts in nude mice. METHODS: The nude mice with CA46 cell xenografts were treated with drugs via intraperitoneal injection daily, and were divided into 5 groups: negative control group, 15 mg/kg baicalin group, 30 mg/kg baicalin group, 60 mg/kg baicalin group and 4 mg/kg etoposide (VP-16) positive control group. After 12-day treatment, the weight of CA46 cell xenografts stripped from some nude mice in the 5 groups was used to evaluate the effect of baicalin on xenograft growth in the nude mice. The apoptosis, necrosis and pathological changes of the xenograft cells were examined under light microscope and transmission electronic microscope respectively. The expression levels of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway-related proteins extracted from xenografts were determined by Western blotting. The other nude mice with CA46 cell xenografts in the 5 groups continued to be treated with the drugs until death in order to evaluate the effect of balcalin on survival time of the nude mice with CA46 cell xenografts. RESULTS: Baicalin remarkably inhibited the growth of CA46 cell xenografts, induced apoptosis and necrosis of xenograft cells, and reduced the protein expression of phospho-Akt (p-Akt), nuclear factor-kappa B (NF-κB), mammalian target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) in the xenografts after 12-day treatment. Furthermore, baicalin prolonged the survival time of the nude mice with CA46 cell xenografts in a dose-dependent manner. CONCLUSION: Baicalin inhibits the growth and induces apoptosis of CA46 cell xenografts in the nude mice, and prolongs the survival time of the nude mice with CA46 cell xenografts through the mechanism of down-regulating PI3K/Akt/NF-κB and PI3K/Akt/ mTOR signaling pathways.     

优质高产不结球白菜新品种“抗寒1号”的选育

"抗寒1号"是以自交不亲和系14LY106为母本,14LY108为父本配置而成的一代杂种。株型较直立,早春播种生长40d时,株高22cm,开展度22cm,叶色绿,叶面平展,叶柄绿,叶柄宽3.2cm,厚0.68cm,单株质量68g,品质脆嫩,味浓,纤维少。每667m~2产量2 400kg以上。该品种抗病毒病、抗霜霉病,抗寒、耐抽薹,适合北方地区早春和秋冬季栽培。     

Characterization of Competitive Mycelial Growth in Pleurotus eryngii

Competitive mycelial growth(CMG)between Pleurotus eryngii isolates is genetically controlled,and stable CMG responses(positive,neutral or negative)were recorded among the strains tested.Correlations between CMG responses and mycelial growth rate were observed.CMG simultaneously reflects information relating to somatic incompatibility and mycelia growth rate in edible mushrooms,and can be readily and reliably applied to studies on genetic polymorphism among this group of fungi.     

60Co-γ辐射对茭白叶片光合特性和荧光参数的影响

分别以50,75和100Gy剂量水平的60Co-γ射线对3叶期的茭白(Zizania latifolia)幼苗进行辐射处理,通过测定其叶绿素含量、光合气体交换指标和叶绿素荧光参数来探讨γ射线辐射对茭白植株光合和荧光生理特性的影响.试验结果表明,75 Gy剂量处理茭白的叶绿素a、叶绿素b和总叶绿素含量降低显著;不同剂量的...     

不同保鲜膜对草莓果实贮藏品质的影响

以草莓"红颜"为试材,以PVC(聚氯乙烯)膜为对照,研究了PE(聚乙烯)膜、PHM(多层PE系)膜、纳米银膜、PVDC(聚偏二氯乙烯)膜等5种保鲜处理在(4.0±0.6)℃贮藏条件下对草莓果实失重率、硬度和可溶性固形物、维生素C、可溶性糖含量及感官品质的影响。结果表明:相较于PVC保鲜膜,PVDC膜与PHM膜能够较好的保持草莓果实的贮藏品质。其中PVDC膜包装可以有效地减少果实水分散失,保持草莓的硬度以及果实可溶性固形物含量;PHM膜包装可以较好的保持草莓果实可溶性固形物、维生素C、可溶性糖含量,维持果实的营养成分。