贮存条件对蛋清S-卵白蛋白形成的影响

为了探讨贮存条件对鸡蛋清S-卵白蛋白形成的影响,该文考察了贮存温度、湿度、二氧化碳浓度及涂膜处理等4个影响因素,并以S-卵白蛋白为变量建立了等价蛋龄预测模型。结果表明,S-卵白蛋白形成与贮存温度呈正相关,高温能加速形成;S-卵白蛋白含量受湿度影响不显著,高湿仅能有限抑制S-卵白蛋白形成;低浓度CO2(2.5%)抑制S-卵白蛋白形成效果不明显,而高浓度CO2(≥5.0%)抑制效果显著;涂膜处理能显著抑制S-卵白蛋白形成,尤其是油溶性、矿物油涂膜处理抑制效果极显著。指数回归模型分析建立了等价蛋龄预测模型,将S-卵白蛋白含量转换为等价蛋龄(25℃),可评价不同条件下贮存鸡蛋的鲜度。     

塔里木沙漠公路防护林咸水灌溉土壤盐渍化状况研究

为了研究塔里木沙漠公路防护林建林后土壤在咸水灌溉条件下的盐渍化状态,本文结合防护林建林前土壤普查资料土壤的盐分状况,运用统计分析、相关性分析及灰色关联分析,对防护林沿线的40个监测点的土壤盐离子、土壤盐渍化的现状及驱动力作了初步研究。研究结果表明：塔里木沙漠公路防护林建成后,土壤盐分各离子含量均较建成前有了显著增加。其中,阳离子含量Na K ＞Mg2 ＞Ca2 ,阴离子含量Cl-＞CO32-＞SO42-＞HCO3-;防护林沿线土壤盐渍化类型以氯化物型与硫酸盐-氯化物型为主,分别占到监测点的84.2%与15.8%;对土壤盐渍化程度的研究表明：有近81.6%的监测点已达到中度盐渍化程度;灰色关联分析结果表明：引起塔里木沙漠公路防护林土壤盐渍化的主要驱动力依次是灌溉年限、地下水矿化度,地下水pH和地下水埋深。     

3MQ-600型导流式气流辅助喷杆弥雾机研制与试验

为减少药液的飘失和雾滴的穿透力,提高药液利用率,设计了3MQ-600型导流式气流辅助喷杆弥雾机,利用拖拉机液压和动力输出带动液压马达、轴流风机、隔膜泵等部件工作。在风筒内部加装的新型栅格状导流器,优化了风筒内的流场结构,减小了因涡流而导致的能量损耗。试验结果证明,与无风幕喷雾相比,沿喷杆方向的雾滴个数平均值提高了29.5%,变异系数降低了78.5%;飘失量减少45%以上;在雾滴对作物冠层穿透性方面,雾滴在作物中下层沉积量50.8%。该机具有较高的减飘性能和雾滴沉积性能,农药利用率更好。     

Nitrogen Fractions and Release in the Sediments from the Shallow Lakes in the Middle and Lower Reaches of the Yangtze River Area, China

Different nitrogen (N) fractions from 14 sediments from the shallow lakes in the middle and lower reaches of Yangtze River area before and after N release experiments were investigated, and the content of different N fractions, and contribution of different N fractions to the N released from sediments were also studied. Ion-exchangeable form (IEF–N), carbonate form (CF–N), iron-manganese oxide form (IMOF–N) and organic matter-sulfide form (OSF–N) accounted for 2.72～17.67%, 0.47～4.43%, 1.18～3.49% and 31.05 to 71.61% to total N, respectively. The N released was higher than 50% from IEF–N, approximately 35% from OSF–N, 6 and 8% from CF–N and IMOF–N on the average. Approximately 27.32～70.02% of IEF–N, 10.37～32.11% of CF–N, 11.37～33.43% IMOF–N and 2.02～8.19% OSF–N were released. For the sediments that were slightly polluted, IEF–N was the main N fraction that may be released and its contribution to total N released was more than 63.07%, for the sediments that TN was higher than 3,540.27 mg·kg^?1, OSF–N would become the main N fraction that can be released and its contribution to total N released was more than 45%.     

中国农产品产地加工产业布局分析及发展对策

通过对大宗农产品的主要产地分布和大宗农产品加工业的产业布局进行分析,可推知中国大宗农产品产地初加工的格局已基本形成。农产品加工各行业的企业规模偏小、投资盲目、企业间无序竞争、精深加工的程度低、产品的附加值普遍不高等问题是制约中国农产品产地初加工产业化的问题所在。因此,应尽快实现中国农产品产地初加工由初级加工向高附加值精深加工转变,基本形成与国际接轨的农产品加工标准体系和全程质量控制体系,初步形成产学研相结合的研发机制和先进适用技术成果的推广体系,为农产品加工业健康快速发展奠定良好基础。     

6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested broccoli

The effect of 6-benzylaminopurine (6-BA) on the color, antioxidant activity, and contents of total phenols, glucosinolate, and sulforaphane in broccoli florets was investigated. The results showed that 6-BA treatment markedly inhibited the increase of the L* value and malondialdehyde (MDA) content and retarded the decrease of the H value. 6-BA treatment reduced the rate of chlorophyll degradation by regulating the activities of chlorophyllase and Mg-dechelatase. When compared to control florets, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) were enhanced in florets treated with 6-BA, whereas the activity of peroxidase (POD) was significantly reduced. The contents of total phenols, glucosinolate, and sulforaphane in broccoli florets were also profoundly increased after treatment with 6-BA. These results indicated that 6-BA could maintain the quality, delay senescence, and improve the nutritional value of broccoli.     

纹杆块与钉齿组合式轴流玉米脱粒滚筒的设计与试验

为解决黄淮海地区玉米直接进行籽粒收获破碎率和未脱净率高的问题,该文在分析现有脱粒滚筒结构特点的基础上,设计了组合式轴流玉米脱粒滚筒,选取滚筒转速、滚筒倾角和凹板间隙为试验因素,在自制的玉米脱粒试验台上进行了单因素试验和正交试验,并运用SAS统计分析软件对试验结果进行了分析。单因素试验结果表明:随着滚筒转速的增大,籽粒破碎率先降低后升高,未脱净率则急剧减小并趋于稳定;随着滚筒倾角的增大,籽粒破碎率和未脱净率则逐渐变小;随着凹板间隙的增大,籽粒破碎率先降低后升高,未脱净率先升高后降低并趋于稳定。正交试验结果表明:影响籽粒破碎率和未脱净率的主次因素顺序均为滚筒转速、滚筒倾角、凹板间隙,且转速430 r/min、滚筒倾角6?和凹板间隙55 mm时籽粒破碎率和未脱净率均最低。该研究可为高含水率玉米脱粒滚筒的设计提供参考。     

Molecular analysis of the major capsid genes (<Emphasis Type="Italic">g23</Emphasis>) of T4-type bacteriophages in an upland black soil in Northeast China

Bacteriophages (phages) are the most abundant biological entities on the planet and are important as the greatest genomic reservoirs in both marine and terrestrial environments. In this study, we analysed T4-type phage communities in an upland black soil by monitoring g23 clones in DNA extracted from seasonal soil samples with no fertilizer, chemical fertilizers, chemical fertilizers plus manure, and natural restoration treatments. PCR products with degenerate primers MZIA1bis and MZIA6 were subjected to denaturing gradient gel electrophoresis. In total, 46 clones with different g23 sequences were obtained. Phylogenetic analyses indicated that T4-type phage communities in the upland black soil were distinctly different from those in marine environments and in an Antarctic lake, which strongly suggested that T4-type phage communities in soil differed from those in aquatic environments. Among 46 clones, 18 clones formed clusters with the clones from rice field soils, 14 clones formed three new clusters, and 13 clones were left as ungrouped, which indicated that T4-type phage communities in the upland black soil were relatively similar to those in rice field soils but that specific communities also inhabit in the upland black soil exclusively.     

玉米倒伏胁迫影响因子的空间回归分析

为指导玉米新品种的推广,采用回归模型分析玉米主产区倒伏胁迫空间分布成因。该文用多元逐步线性回归法筛选黄淮海夏播玉米区的倒伏胁迫的决定因素,比较普通最小二乘法线性回归模型和地理加权回归模型的结果,以确定倒伏胁迫及其决定因素是否存在空间非平稳性和空间依赖性。结果表明：在探索倒伏的空间异质性时,地理加权回归模型显著优于普通最小二乘法线性回归模型;日降水量是玉米倒伏胁迫的主要环境成因,且倒伏程度随日降水量增加而加重;土壤含氮量、留苗密度和日平均风速与倒伏的关系随空间位置而发生正负向变化,因地制宜的分析倒伏成因才能客观有效的指导农民种植生产。     

番茄不孕病毒BJ株系基因组测定与侵染性克隆

番茄不孕病毒(Tomato aspermy virus,TAV)可侵染包括藜科(Chenopodiaceae)、茄科(Solanaceae)等在内的24个双子叶家族和3个单子叶家族的100多种植物,是具有重要经济价值的植物病毒之一.为研究TAV BJ株系(Tomato aspermy virus,TAV-BJ)的基因组功能,本实验对TAV-BJ基因组克隆测序,并构建侵染性克隆.以TAV-BJ侵染心叶烟(ic otiana glutinosa)的总RNA为模板,RT-PCR获得其RNA2和RNA3;以TAV-BJ的dsRNA为模板,RT-PCR获得全长RNA1,目的片段PCR产物克隆测序获得TAV-BJ基因组全序列信息.RNA1全长3 409 nt,编码994个氨基酸的1a蛋白;RNA2全长3 023 nt,含2个开放阅读框(open reading frame,ORF),2a ORF编码829个氨基酸的2a蛋白,2b ORF编码78个氨基酸的2b蛋白;RNA3全长为2 216 nt,包含2个ORF,3a ORF编码247个氨基酸的3a蛋白,外壳蛋白(coat protein,CP)ORF 编码219个氨基酸的CP蛋白(TAV-BJ基因组RNA1、2和3 GenBank登录号分别为HQ424163,HQ424164和HQ424165).TAV-BJ基因组cDNA克隆体外转录成RNA并接种于心叶烟上,结果表明转录产物在寄主上的症状反应和TAV-BJ病毒粒子RNA的接种相一致,TAV-BJ基因组cDNA侵染性克隆具有活性.由TAV-BJ各个基因片段与缺失2b基因的黄瓜花叶病毒Fny株系(Cucumber mosaic virus,CMV-Fny△2b)构建的假重组病毒接种于心叶烟,结果显示TAV-BJ的RNA2和RNA3能恢复CMV-Fny△2b在寄主上症状反应.嵌合型RNA3F3aTcp和RNA3T3aFcp的症状反应结果表明,F1F2△2bRNA3 T3aFcp在寄主上产生花叶症状与F1 F2△2bT3相一致.本研究获得TAV-BJ的基因组序列,成功构建侵染性克隆,同时发现TAV-BJ的3a基因具有CMV-Fny的2b基因的某些功能.