Radioligand binding assay of insulin receptor in rabbit kidney during ischemic and reperfusion

AIM: To observe the change of insulin receptor in rabbit kidney with acute ischemic-reperfusion injury. METHODS: 15 Japanese white rabbits were allocated randomly into control group, ischemic-reperfusion group(IR group). IR group received clamping for 1 h followed by 2 h or 48 h of reperfusion. At 2 h or 48 h after reperfusion, glucose and insulin in serum were determined. Insulin receptor in renal tissue was analyzed by radioligand binging assay(BAD). RESULTS: The level of serum glucose increased after 2 h reperfusion in 2 groups, but in IR group the value increased much more higher than those in control groups(P＜0.05). Plasma insulin of IR group was significantly higher than that in control after 2 h reperfusion(P＜0.05). Scatchard analysis of data resulted in curvilinear profiles, indicating that there are two classes of receptors with different affinity or the presence of a single class of receptors with a negative cooperative hormone-receptor interaction. Data analyzed for a two-site model showed that the values of Bmax₁(high affinity site), Bmax₂(low affinity site) and Kd₁, Kd₂ were significantly lower than that of control (P＜0.05) after 2 h perfusion. 48 h after IR there was no difference of Bmax₁, Bmax₂, and Kd₁ between 2 groups,but Kd₂ of IR group was higher than that of control (P＜0.05). CONCLUSIONS: The results indicate that the effect of intrinsic insulin decreases in the progress of the renal ischemic-reperfusion. The resulting high serum glucose may aggravate renal injury in the progress of ischemic-reperfusion.     

一起未经检疫调运牲畜引发疫情的调查处理

1案情2003年3月23日锡盟正镶白旗改良站从通辽市科左后旗查金台牧场购入26头西门塔尔牛 (其中3头种公牛 ) ,用汽车从科尔沁区运抵锡盟正镶白旗羊场 ,并与2002年11月从通辽科左后旗查金台牧场购入的10头牛混群饲养。24日下午起 ,新购进牛陆续发病 ,至31日病牛已达26头 (新购和原购的牛都有 ) ,旗兽医站接到疫情报告后即派兽医人员到羊场调查情况 ,并采取了相应措施。兽医人员初步诊断为某一类病 ,于是向旗、盟畜牧局和政府上报疫情。并采取病料送自治区兽医站检验诊断 ,确诊为某一类传染病。2处罚经自治区动物防疫监督检验所调查取证 ,这是一…     

青海牦牛肉食用品质及安全性的分析研究

对年龄2周岁左右的12头青海牦牛肉的食用品质及安全性指标进行了系统研究。结果表明：牦牛肉食用品质较好，肉质安全，无污染。具有肌红蛋白含量高、亲水率高、多汁性好，香味浓郁，肉质细嫩，口感适宜等特性。肉中肌红蛋白含量极显著高于黄牛（P＜0．01），肉中重金属元素和有毒有害物质限量均符合GB18406．3和NY5044的要求，是生产优质牛肉制品的原料，也是一种具有较高生物学价值的安全的肉食品，具有很大的开发价值。     

液相色谱-电喷雾质谱联用检测蜂蜜中四种硝基呋喃类代谢物

介绍了高效液相色谱电喷雾多级质谱(LC/MSn) 同时快速、准确测定蜂蜜中呋喃西林、呋喃唑酮、呋喃它酮和呋喃妥英4种硝基呋喃类抗生素代谢物。硝基呋喃类代谢物在酸性条件下经过邻硝基苯甲醛衍生化, 液相萃取后经色谱分离, 利用二级质谱进行定性和定量。加标样品平均回收率达到64% ~79%, 定量下限(LOQ) 为0 1~1μg·kg-1, 检测限(LOD) 达到0 05~0 5μg·kg-1。该方法测定结果满足欧盟(EU)对进口动物源性食品中硝基呋喃类抗生素的残留要求。     

利用血液蛋白及酶多态性分析技术鉴定山羊亲缘关系的研究

选择血红蛋白(Hb)、运铁蛋白(Tf)、亮氨酸胺肽酶(LAP)、前白蛋白3(Pa3)、淀粉酶(Amy)对可能为同卵双生波尔山羊的亲缘关系鉴定。结果发现，除淀粉酶(Amy)和亮氨酸胺肽酶(Lap)外，其余位点均有多态性。由蛋白位点的基因型判定羔1、羔2基因型完全一致，是供体后代的概率为75G，受体后代的概率为22．8％，从而确认羔1、羔2的双亲为供体公羊和供体母羊。     

抗附红细胞体有效药物的筛选

以PCR检测附红细胞体呈现阳性，且镜检染虫率达909／5以上的猪血样为研究对象，选择几种附红细胞体敏感的药物(血虫净，附红净，庆大霉素，博士914，土霉素，三毒清，红弓链914，附红120，红弓链克，乌金土霉素)和对支原体敏感的恩诺沙星，对立克次氏体敏感的红霉素，采用两种方法进行体外药效试验。附红净对附红细胞体的作用效果最明显，而庆大霉素对附红细胞体基本无效，其他药物对附红细胞体也有一定杀灭效果。     

微卫星与金华猪生长性状关系的研究

采用微卫星标记多重扩增结合荧光标记检测技术,对152头金华猪种群的30个微卫星位点的不同基因型与生长发育性状的关系进行了初步分析和显著性检测。结果显示SW 2476、SW 0215、SW 24等3个微卫星位点不同基因型间的出生重差异显著;SW 902、S0143、SW 0215、S0228、SW 24等5个微卫星位点不同基因型间的60日龄重差异显著;S0178、S0218、S0255、S0155、SW 951等5个微卫星位点不同基因型的4月龄重差异显著;而S0217微卫星位点的不同基因型的6月龄重差异显著。     

Multimarker and rare variants genomewide association studies for bone weight in Simmental cattle

Bone weight, defined as the total weight of the bones in all the forequarter and hindquarter joints, can reflect somebody conformation traits and skeletal diseases. To gain a better understanding of the genetic determinants of bone weight, we used a composite strategy including multimarker and rare‐marker association to perform genomewide association studies (GWAS) for that character in Simmental cattle. Our strategy consisted of three models: (i) A traditional linear mixed model (LMM) was applied (Q+K‐LMM); (ii) single nucleotide polymorphisms (SNPs) with p‐values less than .05 from the LMM were selected to undergo the least absolute shrinkage and selector operator (Lasso) in the second stage (LMM‐Lasso); (iii) genes containing two or more rare SNPs were examined by performing the sequence kernel association test (gene‐based SKAT). A total of 1,225 cattle were genotyped with an Illumina BovineHD BeadChip containing 770,000 SNPs. After the quality‐control procedures, 1,217 individuals with 608,696 common SNPs and 105,787 rare SNPs (with 0.001 < minor allele frequency [MAF] <0.05) remained in the sample for analysis. A traditional LMM successfully mapped three genes associated with bone weight, while LMM‐Lasso identified nine genes, which included all genes found by traditional LMM. Only a single gene, EPHB3, surpassed the significance threshold after Bonferroni correction in gene‐based SKAT. In conclusion, based on functional annotation and results from previous endeavours, we believe that LCORL, RIMS2, LAP3, PRKAR2B, CHSY1, MAP2K6 and EPHB3 are candidate genes for bone weight. In general, such a comprehensive strategy for GWAS may be useful for researchers seeking to probe the full genetic architecture underlying economic traits in livestock.     

补饲水平对甘肃高山细毛羊生产性能的影响

为了探讨补饲水平对高寒牧区甘肃高山细毛羊生产性能的影响,本研究选择12月龄甘肃高山细毛后备母羊48只,随机分为补饲Ⅰ组(颗粒饲料)、Ⅱ组[玉米(Zea mays)和苜蓿(Medicago sativa)干草]、Ⅲ组[玉米、苜蓿干草和燕麦(Avena sativa)干草]和对照组(不补饲),进行冬春季"放牧"+"补饲"试验,比较了后备母羊体重,剪毛量,毛纤维长度、细度、白度、强度和伸度等物理指标。结果表明,试验Ⅰ组、Ⅱ组和Ⅲ组试验羊平均日增重分别比对照组高148.29%、133.09%、96.76%;剪毛量各组之间差异不显著(P0.05);自然长度和伸直长度对照组均极显著低于Ⅰ、Ⅱ、Ⅲ组(P0.01),且自然长度Ⅰ组极显著高于Ⅱ组和Ⅲ组(P0.01),伸直长度Ⅲ组极显著低于Ⅰ组和Ⅱ组(P0.01),Ⅱ组显著高于Ⅰ组(P0.05);细度(直径)Ⅰ组最大,Ⅰ组极显著高于对照组(P0.01),且显著高于Ⅱ组(P0.05);白度Ⅰ组最高,Ⅰ组显著高于对照组(P0.05),其他各组之间均差异不显著(P0.05);强度和伸度补饲组均极显著高于对照组(P0.01),且强度Ⅰ组极显著高于Ⅱ组(P0.01),伸度Ⅰ组显著高于Ⅱ组(P0.05)。由此可见,补饲对体重、羊毛品质都有显著正向影响,高寒牧区冬春季节牧草营养缺乏,为了满足羊只营养需要,使甘肃高山细毛羊后备母羊能在1.5岁进行配种繁殖,在冷季放牧的基础上补饲1号料为最佳选择。     

紫花苜蓿细胞质雄性不育恢复基因的初步定位

以紫花苜蓿(Medicago sativa)不育系MS-GN-1A为母本,恢复系MS178为父本组合构建BSA分离群体,获得的F1代均表现为雄性可育,在大田种植了221株F2代群体单株,盛花期将花粉颗粒染色后,显微镜下观察统计出,不育的F2代株数为57,可育F2代株数164,并没有观察到半不育植株。将所有植株划分为可育和不育两组,并构建可育和不育DNA混池,混池DNA从可育和不育植株组DNA中各随机抽取20个样品,以此对恢复基因定位。随机挑选160对已知的四倍体苜蓿SSR引物扩增基因池DNA,获得2个具有多态性的分子标记,分别是Mt2c12、AW166,初步定位Rf基因在类群Composite5上。将类群Composite5上的所有引物进行合成,进一步进行引物筛选,最终获得4个具有多态性的标记BI68、Mt2c12、BG267和AW776153,遗传距离分别为19.0、20.9、44.6和72.1cM。