Experimental study on how brain-dead state affects the heart structure and function of Ba-Ma mini pigs and the mechanism

AIM:To investigate how brain-dead state affects the heart structure and function and the effect of PKC-α in BA-Ma mini pigs.METHODS:Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group (n=5),and control group (n=5). The brain-dead model was made by increasing intracranial pressure,while the control group was maintained anesthesia for 24 h. The concentrations of cTnT,TNF-α,IL-1β and IL-6 in serum were determined at 6,12 and 24 h after brain death. At 24 h,heart tissues were observed by HE staining and electron microscope. The expression of PKC-α was detected by immunohistochemistry and RT-PCR.RESULTS:(1) Histological changes of myocardium: flaky bleeding under endocardium and dissolution of myocardium were found in optical microscope. In electron microscope dropsical mitochondria and confluent muscle fiber were found. (2) Changes of serum cTnT: serum cTnT for brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (3) Changes of inflammatory factors: IL-1β,IL-6,and TNF-α in brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (4) Changes of PKC-α expression: PKC-α mRNA and protein expressions in brain-dead group increased significantly at 24 h (P<0.05).CONCLUSION:Brain death may evoke heart structure and functional injury,and increase the levels of inflammatory factors and PKC-α. The activation of PKC-α may participate in the process of heart injury.     

Sequence analysis and prokaryotic expression system construction of PIA genes isolated from Neisseria gonorrhoeae

AIM: To analyze the nucleotide and putative amino acid sequences of PIA genes isolated from N.gonorrhoeae and to construct the prokaryotic expression system of PIA gene.METHODS: The entire PIA genes from 9 strains of N.gonorrhoeae were amplified by using high fidelity PCR.The target amplification fragments were sequenced after T-A cloning.Homology comparison of the nucleotide and putative amino acid sequences of PIA genes from the isolates with the reported sequences in GenBank was then performed.A prokaryotic expression system of PIA gene was constructed.Different dosages of IPTG were applied to induce the expression of the target recombinant protein (rPIA) and 10% SDS-PAGE plus Bio-Rad Agarose Image Analysor was used to determine the expression level of rPIA.rPIA was extracted using Ni-NTA affinity chromatography and the purified effect was detected by SDS-PAGE.RESULTS: In comparison with the reported PIA gene sequences (GenBank No: L19962),the homologies of nucleotide and putative amino acid sequences of PIA genes from the isolates were 99.6%-100% and 99.1%-100%,respectively,which indicated that all the isolates were belonging to serovars IA6.Output of rPIA was as high as 50.1% of the total bacterial proteins.The purified rPIA only showed a single target protein fragment in gel.CONCLUSION: Serovar IA6 is dominant in the local N.gonorrhoeae isolates and sequences of the encoding gene are relatively conserved.The constructed prokaryotic expression system is able to express rPIA with high efficiency,which may lay a foundation for further development of serological detection kit and vaccine of N.gonorrhoeae.     

万寿菊细菌性叶斑病药剂防治试验

通过室内抑菌筛选和田间小区及生产试验,从当前防治细菌性病害的药剂中,筛选出对万寿菊细菌性叶斑病防治效果较好的3种药剂及最佳使用浓度:90%新植霉素150mg/L,细菌清200 mg/L和72%农用链霉素150 mg/L,其防效分别为73.6%,72.7%和70.7%,且药效稳定,增产效果显著.     

江苏省扬中杂草稻生物学特性初步研究

扬中杂草稻具备杂草稻的基本特性，又是一个具有遗传多样性的混合群体。扬中杂草稻具有较强的休眠性，但比稽稻等代表性的杂草稻弱。扬中杂草稻落粒性强，与稽稻等代表性杂草稻表现基本一致。亲和性研究表明，扬中杂草稻与广亲和品种02428（S5^n）正反交杂种F1结实率正常，而与籼稻特青和扬稻6号的杂种F1生育期超亲，在南京不能正常抽穗。与粳稻武运粳7号、粳稻品系27114的杂种F1表现为半不育。     

家天牛在中国的适生区分析

家天牛原产于非洲北部的阿特拉斯山脉,是木材和具木质结构建筑物的重要害虫。本文运用CLIMEX气候模型的气候相似系数法对该虫在中国的适生区进行了分析和预测。结果表明,中国大陆有21个省区33个市、县(地区)适合该虫生存,大体分布于23.22°N～40.59°N,99.09°E～123.32°E范围内。     

奶牛蹄病的病因与综合防治

奶牛蹄病是指奶牛蹄部的病理变化,包括蹄病和蹄变形。奶牛蹄病是奶牛业的主要疾病之一,与乳房炎和生殖系统疾病共同构成奶牛饲养业的三大制约因素。奶牛蹄病是奶牛的常见疾病,从疾病的病理表现分为蹄底溃疡、白线损伤、蹄底脓肿、趾     

原木灵芝孢子研究：（Ⅲ）与木屑袋栽灵芝孢子的鉴别

原木灵芝孢子与木屑袋栽灵芝孢子可以鉴别如下:1.在扫描电镜下,前者形态饱满,后者大多数孢壁内陷;2.X-射线能谱面扫描分析结果表明,两者Mg、Al、Si、P、S、Cl、K、Ca、Fe、Ni等无机元素相对含量差异显著,前者Si、Ca含量较高;3.高效液相色谱分析结果表明,前者灵芝酸(Ganoderic acid)A、B等灵芝三萜酸含量较高,而后者未检出Ganoderic acid C;4.孢子粉经乙醚脱脂后用乙醇回流提取、浓缩成浸膏,前者提取率(固形物含量)达4.2％～4.8％,而后者仅为1.9％～2.1％。     

不同生态区藏山羊的产绒性能研究

选择青藏高原四个不同的生态区,对藏山羊的产绒性能进行了研究,结果表明：西藏两个产区藏山羊的产绒量和绒的主要品质指标均高于四川两个产区,尤其是西藏阿里地区藏山羊的产绒量和绒的品质明显优于其他产区,四川茂县藏山羊的产绒量和绒的品质较差;同时,西藏两产区藏山羊的产绒量从年龄上看以４岁较高,从毛色上看以白色个体较高;四个不同生态区内藏山羊的产绒性能差异较大,越严酷的高寒生态条件,其产绒量越高,绒的品质越好。今后应根据不同的生态区按不同的生产方向对藏山羊这一宝贵的基因库资源进行开发利用。     

传染性法氏囊病病毒抗体检测诊断试剂盒的研制与应用

本文用提纯的ＩＢＤＶ细胞毒为抗原,抗鸡Ｉｇ单抗１Ｂ７ＨＲＰ酶结合物为第二抗体建立了检测ＩＢＤＶ抗体的间接ＥＬＩＳＡ。在此基础上研制出ＩＢＤＶ抗体检测诊断试剂盒。经对１２００份血清进行检测,结果表明：本试剂盒特异性强,灵敏度高,重复性好,快速稳定,易于操作,为雏鸡ＩＢＤＶ母原抗体和疫苗注射后特异性抗体检测提供了可靠的诊断工具。     

鄂温克旗退化草地现状与草业的可持续发展

通过对鄂温克旗天然草地资源23年(1974～1997)的动态分析,阐明了自治旗天然草地退化的现状、特征、趋势和导致草地退化的主要原因,并提出了相应的可持续发展措施.