2004年冬成都市外来园林植物冻害调查及建议

作者2005年春调查了成都市外来园林植物冻害情况,发现：（1）外来园林植物受冻害影响较为普遍,41种植物在不同程度上受害;（2）冻害影响存在区域差异,郊区冻害比城区严重.分析了冻害原因,并对园林植物引种提出建议.     

蜂花粉营养液的营养成分分析

蜂花粉营养液以油菜花粉和蜂蜜为原料,经过先进的生产工艺制备而成.本文主要对其营养成分进行了分析,该营养液的营养成分搭配合理,含有17种氨基酸,其中包括8种必需氨基酸,氨基酸总量为575.2 mg/100mL;钙31.90 mg/100mL、钾77.46 mg/100mL、钠6.16 mg/100mL、镁19.12 mg/100mL、锌0.14 mg/100mL、铁0.14 mg/100mL、锰0.13 mg/100mL、铜0.053 mg/100mL;尼克酸0.24 mg/100mL、维生素B2 0.01mg/100mL、维生素B6 0.74 mg/100mL、维生素C 0.75 mg/100mL.     

中国山羊品种资源及其遗传多样性研究

本文论述了中国山羊的起源、品种遗传资源及其遗传多样性的研究进展。最新调查 显示中国的山羊品种达50个,其中43个为地方品种,4个培育品种,3个引入品种,许多品种 具有独特的生产性能或适应能力。大量研究表明,我国山羊遗传多样性较为丰富。     

辣椒红色素的检测方法

综述辣椒红色素的多种检测方法,即溶解性试验、显色反应、分光光度测定法及薄层层析法。     

乌梁素海湿地环境与资源地理信息系统的研究

本系统建立乌梁素海湿地基础地形库、生态库、资源库、工程档案库、遥感影像库等基础数据库及信息发布系统 ,使 GPS、GIS、RS紧密结合 ,同时实现了专家决策支持系统与地理信息系统之间的一体化集成。本系统可以为乌梁素海湿地生态治理与资源开发提供全方位的数据信息及其管理 ,对生态工程规划与实施方案进行优化设计 ,并提供实时监控服务。     

The mechanism of neuronal injury and repair after focal cerebral ischemia

AIM:To explore the mechanism of neuronal injury and repair by investigating the expression of caspase-3 and apurinic/apyrimidinic endonuclease (APE/Ref-1) after focal cerebral ischemia. METHODS:A model of middle cerebral artery occlusion in rats was performed. The expression of caspase-3P₂₀ and APE/Ref-1 was examined by immunohistochemistry staining, TUNEL was applied to detected DNA damage, and double labeling with TUNEL and APE/Ref-1 was used to determine the relationship between APE/Ref-1 and DNA damage. RESULTS:The active subunit P₂₀ of caspase-3 was predominantly expressed within ischemic penumbra. The peak time of caspase-3P₂₀ positive cells preceded the appearance of TUNEL. With aggravation of cerebral ischemia, APE/Ref-1 immunoreactive cells in penumbra were significantly decreased. CONCLUSION:The activation of caspase enzymatic cascade following cerebral ischemia leads to degradation in DNA, meanwhile, decrease in DNA repair molecules or the failure of DNA repair may deteriorate the course.     

Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat

AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P＜0.01), 2.3 folds at 2 nd week(P＜0.01) and 1.7 flods at 4 th week(P＜0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P＜0.01), 1.9 flods(P＜0.01) and 1.4 folds(P＜0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling.     

Effects of Latent Infection of Stock Plants and Abundance of Thrips on the Occurrence of <Emphasis Type="Italic">Tomato spotted wilt virus </Emphasis>in Chrysanthemum Fields

DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001     

黑河干流中游地区耗水量变化的历史分析

黑河流域水资源极其匮乏 ,加之地区上的配置不均衡和中游地区水土资源的过度开发导致了黑河下游严重生态环境问题 ,已影响到我国北方广大地区生态环境质量 ,受到国家的高度重视和全社会的广泛关注。黑河水资源开发利用主要集中在中游地区 ,该区是全国重要的商品粮、蔬菜和经济作物生产基地之一 ,耗水量占流域耗水量的 85 %以上 ,是黑河的主要耗水区和径流利用区。本文利用黑河上游控制站莺落峡站和中游控制站正义峡站水文资料 ,分析了黑河中游耗水量的变化 ,结果表明 :2 0世纪 80年代以前黑河中游耗水量在 4.2 0× 10 8m3 左右 ,比较稳定 ;进入 2 0世纪 80年代以来耗水量明显增加 ,80年代耗水量比 40年代末期至 70年代增加了 2 .0× 10 8～ 2 .5×10 8m3 左右 ;90年代耗水量又比 80年代增加了 1.6 0× 10 8m3 左右。 80年代以来流域耗水量呈明显增加的趋势 ,这是以人类活动影响为主的结果     

苏云金芽孢杆菌cry基因在大肠杆菌中表达产物和生物活性

研究了几种Bt cry基因于大肠杆菌(Escherichia coli)中表达产物在pH 10.0的50mmol/L碳酸钠和20mmol/L乙醇胺溶解液中的溶解性 ,发现同样的Cry蛋白在碳酸钠中的溶解度大于乙醇胺。通过胰蛋白酶消化 ,明确Cry1Ca7、Cry1Ia8酶解产物为 38kD多肽 ;Cry1Ie1、Cry1Cb2、Cry2Ab4酶解产物为 41kD多肽 ;Cry1Ac酶解产物为60kD多肽。采用FPLC层析方法对 6种原毒素及其酶解后得到的毒素多肽进行了分离纯化 ,比较了原毒素和毒素的杀虫活性的差异。其结果表明 ,Cry1Ac的原毒素和毒素对棉铃虫初孵幼虫的校正死亡率均为 100% ,Cry2Ab4的原毒素的毒力高于其酶解毒素。