Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

纳米氧化锌对断奶仔猪生长性能及腹泻率的影响

本试验通过研究纳米氧化锌(Nano-ZnO)对断奶仔猪生长性能及腹泻率的影响,旨在进一步探寻较低剂量纳米氧化锌替代高锌的可行性.将135头23日龄断奶仔猪按照体重相近、遗传基础相似的原则分为5个处理组,每个处理组3个重复,每个重复9头,用纳米氧化锌和高剂量氧化锌(ZnO)进行饲养对比试验,试验期24 d.其中第1组为处理1组(0 mg/kg),第2,3,4组分别为200,400,600 mg/kg纳米氧化锌组,第5组为高锌组(3000 mg/kg ZnO).结果表明较处理1组(0 mg/kg)而言,无论是纳米氧化锌(200,400,600 mg/kg)还是高锌组(3000 mg/kg ZnO)均可极显著提高日增重(ADG)(P＜0.01);400 mg/kg纳米氧化锌组与高锌组(3000 mg/kg ZnO)在日增重方面差异不显著(P＞0.05).400 mg/kg纳米氧化锌降低腹泻率效果最好,较对照组(0 mg/kg)腹泻率降低49.1%,达到极显著性差异(P＜0.01),较高锌组(3000 mg/kg ZnO)腹泻率降低21.6%,达到极显著性差异(P＜0.01).根据ADG、平均日采食量(ADFI)、料肉比(F/G)、腹泻率等指标综合得出,第3组(400 mg/kg Nano-ZnO)可以达到甚至超过第5组高锌组(3000 mg/kg ZnO)效果.     

花粉主要营养成分与保健功能

6 眼病与蜂花粉 眼视力一般随老年人年龄增加而下降。研究发现，眼疾与B族维生素和维生素c密切相关。另外，白内障是老年人致盲的主要原因。尽管目前原因尚不十分了解，但与老年人晶状抗氧能力减弱和脂类过氧化增强有关。     

貂,貉,犬病毒性肠炎快速诊断试剂的研究

用金黄葡萄球菌Ａ蛋白（ＳＰＡ）协同凝集方法（ＣｏＡ）对貂、貉、犬病毒性肠炎快速诊断的试剂进行了制备，确定了制备。艺及该制剂的标准操作程序。本制剂特异性强，不与健康动物的组织及其他几种病毒病（犬瘟热、犬传染性肝炎、狂犬病等）发生交叉凝集反应，灵敏度高，通过该制剂ＣｏＡ法与ＨＡ－ＨＩ法比较结果比现行的貂、貉、犬病毒性肠炎ＨＡ－ＨＩ诊断方法检出率高出３０％，同时具有微量、操作简便、易掌握、快速等特点，可在３～５ｍｉｎ获得诊断结果。     

Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed

One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.     

伊克昭盟牧草和饲料作物应用增产菌技术的试验

199年伊克昭盟引入了牧草和饲料作物增产菌这一技术,经过7年的试验,示范及推广,至今全盟该增产菌应用面积已达4．04万hm^2,本文通过7年来在两种不同生境下,对12种牧草及作物的不同物修期采用不同方法施用增产菌,以鲜草产量和种子产量为衡量标准来探讨增产菌对不同牧草及作物的增产效果,实验结果表明：增产菌技术的应用,促进了植物的生长,使植株长高,叶面积增大,加强了光合作用,在提高鲜草产量的同时也增加了籽实的产量,具有较强的推广价值,是一项在农牧区适用的增产技术。     

巨型艾美耳球虫感染鸡空肠粘膜肥大细胞数量和组织胺含量的变化

本文借助于改良甲苯胺蓝染色法和荧光测定法，分别检测了巨型艾美耳球虫感染鸡空肠粘膜肥大细胞（ＭＭＣ）数量和组织胺（ＨＡ）含量的变化。结果表明，初次感染巨型艾美耳球虫之后，空肠ＭＭＣ呈减少趋势，至感染后第１４天回复至不感染对照组水平。而初次感染组相应空肠ＨＡ水平呈现出不显著波动后，在感染后第４天达最大值。在再次攻毒试验中，免疫攻毒组ＭＭＣ呈现水升趋势，尔后回复至不感染对照组水平；免疫攻毒组织相应肠道Ｈ     

国际金融危机对粮食价格走势及上下游行业的影响

当前．国际金融危机的深化,正在对国内外粮食市场的供求格局和粮价走势产生深刻影响。而对国内市场粮价及粮食上下游企业的影响已经显现．今后将会日趋加重。今后几个月内国内粮价上涨的动力正在迅速弱化．下行的压力却在显著加大．但不排除半年乃至9个月后,国内外粮食价格出现较大反弹的可能性。     

基于AHP-CRITIC复合加权法评价不同发育期苦水玫瑰品质

运用AHP-CRITIC复合加权法评价不同发育期苦水玫瑰的品质。以不同发育期的苦水玫瑰为研究对象,测定总多糖、总黄酮、总多酚含量及抗氧化活性,采用AHP-CRITIC复合加权法综合评价不同发育期苦水玫瑰的品质。结果发现抗氧化IC50、总黄酮、总多酚、总多糖4个指标成分的复合权重分别为0.294 1、0.152 3、0.158 8、0.394 8;幼蕾期、花蕾期、盛开期苦水玫瑰质量综合评分分别为54.362 0、38.451 9、33.801 9,即幼蕾期苦水玫瑰的质量最好,花蕾期次之,盛开期较差。说明通过化学成分和生物活性作用综合评价不同发育期苦水玫瑰的品质,幼蕾期苦水玫瑰品质最好。     

木槿药学研究概况

在广泛检索文献的基础上,综述了木槿的成分、药理及临床应用,为木槿的深入开发利用提供参考依据。