内蒙古地区扁蓿豆酯酶同工酶多样性及其地理分布的研究

利用聚丙烯酰胺凝胶电泳技术,对内蒙古不同生境条件下生长的扁蓿豆的种子酯酶同工酶多样性进行了研究.结果表明,内蒙古地区扁蓿豆的种子酯酶同工酶酶谱具多样性;同一种或种下分类单位,由于材料来源不同,所表现的基本酶谱型与地理分布有关,基本上与草地区划中的地段相吻合.因此,在进行材料收集时应以地段为单位进行,而在地形单元和植被组合变化较大的地段需要配合地片进行.     

ELISA对动物舍气载魏氏梭菌型别的研究

采用ELISA方法对从一个犊牛舍空气中分离到的魏氏梭菌进行型别的鉴定。结果显示：88％的菌种属于A型，3.4％为C型，8.6％是D型，B型未能确认。     

DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ) as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects(No.1-20) had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples(No.21-22) had high difference degree(8.6%) with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.     

东北虎（Panthera tigris altaica）个体识别技术研究进展

对东北虎种群的科学监测是采取有效保护、管理及恢复措施的基础,而准确的个体识别又是保证有效监测的前提。尤其是在中国,在野生东北虎种群数量很少,种群密度极低的情况下,能根据它留下的各种信息进行个体识别就显得尤为重要。目前,用于野生东北虎个体识别的技术主要有：足迹识别、DNA识别、条纹识别以及气味识别等。本文对这几种技术的特点、研究进展、应用注意事项等做了介绍,并且结合中国的实际情况,分析每种技术在中国的适用性,认为如果要建立中国的东北虎种群数量数据库,足迹识别和DNA识别比较适合在中国现有东北虎分布区推广使用。通过足迹识别,信息容易获取,也是最经济和比较成熟的方法;由于种群密度低,含DNA的遗传样本虽不能每次都采集到,但可以长期积累;现阶段条纹和气味识别只能起到辅助的作用。     

重庆市水产品质量安全现状及对策

我国是世界上最大的渔业生产国,其养殖产量占全世界养殖产量的2/3。我国也是水产品出口大国,2006年水产品出口量为301.5万吨,出口额93.6亿美元,占农产品出口总额的     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.     

病原疑似摩拉克氏菌属的禽病调查研究

对不发酵的革兰氏阴性杆菌群疑似摩拉克氏菌属的禽病流行病学,临床症状,病理剖检,病原分离,病原的初步鉴定及致病性试验进行了有系统的调查。对病学和诊断进行了研讨。     

35种蜂花粉的活性物质分析

活性物质一般是指酶、维生素以及激素等,近年将磷脂也纳入活性物质。本文分析了３５种蜂花粉中的葡萄糖氧化酶、磷脂以及生长激素等,这些物质对人体均有重要的生理作用。研究结果,除证明花粉中含有葡萄糖氧化酶及磷脂外,还发现了花粉含有人体生长激素     

山羊霉稻草中毒病例报告

冬春季节青绿饲料缺乏,我县农户多以干稻草饲喂牛羊,若收获季节多雨潮湿,稻草易发霉变质,牛羊长期饲喂霉稻草易引起中毒.2003年春季,我县灌阳镇一养羊户因长期饲喂霉稻草,引起以"烂蹄、坏耳、坏尾"为特征的山羊病例,先后死亡23只,死亡率为46%.山羊霉稻草中毒病例较少见,现将诊治情况汇报如下.     

从牛胃中分离微生物发酵秸秆的研究

试验目的主要是模拟反刍动物发酵作物秸秆多元菌,提高作物秸秆的利用率。通过对稻草秸秆的微贮发酵,提高秸秆的适口性和消化率,而且经过微贮的秸秆饲料成本低、污染少、效率高、经济、安全且利于工业化生产。与单纯饲喂秸秆饲料或饲喂氨化饲料相比,微贮饲料可分别使肉牛增质量提高22.59和8.64kg,增质量速度提高28.33%和8.44%,育肥效果显著,经济效益明显,可操作性强。微贮秸秆技术的推广与应用,不仅可以解决我国大量秸秆资源利用效率不高这一难题,而且可以减少环境污染,促进农业生产和农村经济的发展,增加农民收入,改善人民的肉食结构,提高人民的生活水平,并且为建立节粮型畜牧业结构,更好地发展可持续生态畜牧业做出巨大贡献。