不同产地南果梨果实品质比较

用5个主要南果梨产区的南果梨进行试验,重点测果实糖分组成.结果表明:南果梨果实的糖分组成以果糖为主,占总糖的50%以上;不同产地南果梨果实总糖、可滴定酸、Vc及糖组分含量差异较大,阜新地区南果梨总糖及果糖含量最高,分别为190.27、99.65mg/mL,海城地区南果梨总糖及果糖含量最低,分别为97.74、60.24mg/mL,虽然鞍山地区是南果梨的优势产区,但鞍山地区的南果梨总糖及果糖含量却不是最高的,这个结果与生产中反映的鞍山南果梨果实品质近年来有所下降相符合.     

苹果梨授粉亲和性的研究

以辽宁省果树所试验园14个梨品种花粉和沈阳市苏家屯区双翼苹果梨公司的10年生苹果梨树为试材,探讨了14个梨品种对苹果梨授粉的亲和性。结果表明,在环境条件和栽培条件一致、树体发育正常的情况下,在不同的梨品种之间,花粉发芽率存在着明显的差异;在14个授粉品种和被授粉品种苹果梨之间,存在着授粉亲和性的问题,华酥、早酥、雪花和鸭梨给苹果梨授粉亲和性好,花序坐果率达80%以上;安梨为雄性不育类型,不能用作授粉树。     

七带石斑鱼胚胎及仔稚鱼形态观察

对七带石斑鱼胚胎和仔稚鱼发育过程进行了观察,描述了从受精卵到仔稚鱼各发育时期的形态特征;在水温22±0.5℃、盐度30条件下进行七带石斑鱼仔鱼的饥饿耐受力实验,记录了饥饿条件下初孵仔鱼的存活与生长、卵黄囊与油球的利用情况。结果表明,胚胎发育可划分为卵裂期、囊胚期、原肠胚期、神经胚期和器官形成期。在水温20.5±0.5℃、盐度30.0条件下,受精卵历时38h45min孵化出膜。初孵仔鱼全长1.059±0.071mm,至4日龄全长2.27～2.36mm时,卵黄囊完全消失;16日龄,全长4.99mm时,鳔形成;至25～30日龄,尾鳍鳍条发育完整。在饥饿条件下,初孵仔鱼的死亡高峰出现在孵化后4～6d,半数死亡时间出现在5d,至7d饥饿仔鱼全部死亡。卵黄囊期仔鱼的生长可分为3个阶段:仔鱼初孵时的快速生长期,卵黄囊消失前后的慢速生长期,以及在不能建立外源性摄食后的负生长期。随着生长发育时间的延长,饥饿仔鱼与正常条件下仔鱼的生长差异显著(P<0.05)。饥饿仔鱼体长较短,头大且体瘦,长期饥饿后脑后部下陷。     

5个梨品种在熊岳地区的生长表现及果实性状评价

为优化熊岳地区梨品种结构,同时为梨育种工作提供前期准备,2009年,辽宁省果树科学研究所梨课题从吉林省农科院果树所引进5个抗寒晚熟梨新品种‘蔗梨’‘寒红’‘寒香’‘寒露’和‘寒酥’,通过多年的生物学调查和室内测试分析,比较其物候期、生长结果习性和果实品质,认为‘寒红’‘寒香’‘蔗梨’在熊岳地区表现较好,可以适当发展,同时可作为育种材料在今后的杂交育种中应用.     

牛性控体外受精冷冻胚胎移植试验

从美国引进的荷斯坦牛性控体外受精冷冻胚胎95枚,以鲁西黄牛、南阳牛、西杂牛、荷斯坦牛为受体进行移植试验.结果显示,移植受体95头,妊娠44头,妊娠率46.3%,流产7头,产母犊37头,效果良好.     

Effects of nacetylcysteine on cigarette smoke-induced airway inflammation

AIM: To study the effects of nacetylcysteine on cigarette smoke-induced airway inflammation. METHODS: SD rats were divided into three groups randomly: control, cigarette smoking and cigarette smoking plus nacetylcysteine (NAC). Animals were treated for four weeks. Airway inflammation was assessed with light microscope. The concentrations of IL-8 and glutathione (GSH) in bronchoalveolar lavage fluid (BALF) were measured by ELISA. The expression of nuclear factor-κB (NF-κB) in the cells of BALF was detected with EMSA. RESULTS: Airway inflammation in NAC treatment group was significantly decreased than that in cigarette smoking group. GSH levels in BALF in NAC treated groups were significantly higher than those in cigarette smoking group. IL-8 levels in BALF in NAC treated groups were significantly lower than those in cigarette smoking group. Expression of NF-κB in the cells of BALF was much lower than that in the cigarette smoking group. CONCLUSION: NAC not only increases oxidative capacity in the bronchial, but also reduces the express of NF-κB, which inhibits the IL-8 production. At last it further reduces the airway inflammation.     

梨SRAP体系的优化及其在杂交后代中的分离方式

采用正交设计建立并优化了梨的SRAP-PCR分子标记技术体系，在20 μL的反应体系中含有DNA15 ng/mL，Primer o.5μmol/L，dNTPs 0.1 mmol/L，Mg2+2 mmol/L，Taq DNA聚合酶1.5 U。应用20对引物组合对SRAP标记在“红巴梨×南果梨”F1群体的多态性和分离方式进...     

Effects of drynaria total flavonoids on serum levels of leptin,interleukin 6, prostaglandin E2 and expression of bone β2-adrenergic receptor in ovariectomized osteoporosis rats

AIM: To investigate the effects of drynaria total flavonoids on serum levels of leptin (LEP), interleukin 6(IL-6), prostaglandin E₂(PGE₂) and the expression of bone β₂-adrenergic receptor (ADRB2) in a rat model of ovariectomized osteoporosis(OP). METHODS: The osteoporosis model was established by ovariectomy. Twelve weeks after modeling,bone mineral density (BMD) was determined by dual-energy X-ray absorptiometry to verify successful modeling.Enzyme-linked immunosorbent assay was applied to detect the concentrations of LEP, IL-6 and PGE₂ in serum. The expression of ADRB2 was determined by immunohistochemical technique. RESULTS: Compared with sham group,BMD of the rats in model group significantly decreased in multiple regions 12 weeks after modeling(P<0.01). The serum levels of LEP, IL-6 and PGE₂ in model group were significantly higher than those in sham group(P<0.05). The levels of LEP, IL-6 and PGE₂ in drynaria total flavonoids group were significantly lower than those in model group(P<0.01). No significant difference of PGE₂ between these 2 groups was observed. The ADRB2 expression in sham group and treatment group was significantly different from that in model group, and no significant difference between sham group and treatment group was found. CONCLUSION: The serum levels of LEP, IL-6 and PGE₂ and the expression of bone ADRB2 increased in OP rats.Drynaria total flavonoids reduce the production of LEP, IL-6 and the expression of ADRB2, and suppress the bone absorption, which may be one of the mechanisms in treating OP.     

果蔗新品种引种品比试验

以当地果蔗主栽品种黑皮果蔗为对照品种,对引进的闽引黄皮果蔗、大田雪蔗和同安果蔗进行品种比较试验,结果表明：闽引黄皮果蔗表现出植株高大、茎粗、外观品质好、高产稳产、甜度适中、口感好等特性,具有较好的推广应用前景。     

挤压膨化甘薯粉生产燃料酒精试验

以膨化甘薯粉为原料,通过单因素试验研究不同料水比、糖化酶用量、糖化时间、酵母接种量等几个主要因素对挤压膨化甘薯粉酒精发酵的影响。试验结果表明,挤压膨化甘薯粉生产燃料酒精的最佳条件为料水比1.0∶2.0、糖化酶用量110 U/g、糖化时间30 min、酵母接种量0.10%。在该工艺条件下,发酵醪酒精体积分数达到14.92%(v/v),与传统酒精发酵工艺相比,酒精体积分数提高了3.07个百分点。外加氮源对发酵没有明显的作用。