五节芒的分化类型及生产性状的比较研究

通过长期的引种栽培,发现五节芒分化出宽叶和窄叶类型。经对形态特征、生产性状的比较研究,证明两个类型差异显著,性状稳定。还证明,宽叶类型的生产性状明显优于窄叶类型。     

成都麻羊乳上皮黏蛋白MUC1的遗传多态性研究

采用SDS-PAGE研究成都麻羊乳上皮黏蛋白MUC1的遗传多态性,并以金堂黑山羊、乐至黑山羊、自贡黑山羊、藏山羊作对照进行比较分析。结果表明:成都麻羊与对照山羊乳MUC1的多态性丰富,成都麻羊有7种基因型、5个等位基因优势基因为C、D、E,金堂黑山羊7种基因型、6个等位基因优势基因为B、C、D,乐至黑山羊6种基因型、6个等位基因优势基因为B、C、D,自贡黑山羊5种基因型、5个等位基因优势基因为A、C、D,藏山羊3种基因型、3个等位基因优势基因为C、D。乳MUC1的基因型、等位基因的分布品种间有差异;根据乳MUC1等位基因频率对供试山羊品种进行聚类分析,成都麻羊与金堂黑山羊的遗传距离最小(D=0.2507)先聚为一类,再依次与乐至黑山羊(D=0.2831)、自贡黑山羊(D=0.3721)、藏山羊(D=0.4752)聚为一大类,结果较好的反映了成都麻羊与对照山羊品种间的亲缘关系。     

龙头企业与农户利益联结机制的比较研究——根据对四川省畜禽产业链的调查

根据对四川省畜禽龙头企业、合作社及养殖户的实地调研,发现不同的产品特点和产业发展阶段形成不同利益联结机制,这些机制具有不同的约束和激励效果,企业和农户的风险分担和利益分配关系也不同,并决定企业对原料产品质量安全水平的不同控制力。     

可怕的“生物入侵”

１ 生物入侵危害惊人 生物学家认为，生物入侵比直接危害人类健康的疾病更可怕。给英国养牛业造成毁灭性打击的疯牛病现在在比利时、法国、德国、爱尔兰、瑞士和葡萄牙均有报道。口蹄疫是一种全球范围内最具感染性的动物疾病之一，且很难被消灭。这种病毒主要通过直接接触或通过消化道、呼吸道传染，包括羊，牛和猪在内的偶蹄动物可以感染这种病毒。此病危害性极大，１９９７年台湾屠宰了３８０万头猪，损失达数十亿美元；韩国、日本和阿根廷则损失了大约７０万头牛。 西尼罗河脑炎是一种人兽共患病，已造成纽约地区７人死亡。这种病毒源朗…     

贵州喀斯特地区牧草引种试验评价

对国内外引进的6个禾本科牧草品种,9个豆科牧草品种以及喀斯特地区的4个对照品种,在贵州独山地区进行引种试验。结果表明：各参试品种生长良好,能安全越冬、越夏,一年可刈割5～6次,鲜草产量达36558．2～66450．0kg／hm2,干草产量10771．0～19869．0kg／hm2。综合性状表现较好的依次为鸭茅Totter,Wana,Tuoa;多年生黑麦草Nui,Bronsys;白三叶Alice,C6151,Prop,Pitua,Sustain;百脉根Mareb,Lel。     

有机铬对奶牛的作用

奶牛在妊娠晚期、产奶早期、高温时期和运输过程中处于极度应激的状态下,生产性能、免疫力、繁殖性能均受到影响。近年来研究发现有机铬对缓解奶牛应激有显著的作用,本文将就此作一综述。     

规模化肉鸭场污水污染物排放及其影响分析

为了解规模化肉鸭场污水污染物排放及其对水环境污染现状,本试验选择河南省5个有代表性的规模化肉鸭场,对其所排放污水以及附近水体中的化学需氧量(COD)、五日生化需氧量(BOD5)、氨氮(NH3-N)、总磷(TP)、悬浮物(SS)等污染物含量进行了分析,并利用单因子污染指数法对其污染程度进行评价.结果表明:鸭场排放污水中COD、BOD5、NH3-N、TP、SS的平均含量均超出《畜禽养殖业污染物排放标准》.其中COD、BOD5、NH3-N、TP属于较严重污染,而SS属于严重污染.鸭场下游水体中COD、BOD5、NH3-N、TP的平均含量均高出《地表水环境质量标准》,其中COD、BOD5、TP污染较严重,而NH3-N为严重污染.因此,规模化肉鸭场污水排放污染物指标全部超标,并且排放的污水对周围水环境造成不同程度的污染.     

Effect of Fagopyrum dibotrys (D.Don) Hara on Growth Performance,Immune Function and Intestinal Structure of Broilers

This experiment was conducted to investigate the effects of Fagopyrum dibotrys (D.Don) Hara on growth performance,immune function and intestinal structure of broilers. A total of 270 one-day-old healthy Tiejiaoma broilers were chosen and randomly divided into 3 groups with 6 replicates per group and 15 broilers per replicate.Control group was fed with the basal diet,group Ⅱ was fed with basal diet adding 8% flavomycin 60 mg/kg +15% aureomycin 270 mg/kg and group Ⅲ was fed with basal diet adding 1% Fagopyrum dibotrys (D.Don) Hara. The trial period lasted for 21 days. The results showed as follow:① At 21 days of age,the average daily feed intake of group Ⅲ was significantly lower than that of groups Ⅰ and Ⅱ (P<0.05).However,there was no significant difference in the average daily gain and F/G among the three groups (P>0.05).②At 21 days of age,the thymus and bursa of Fabricius indexes of groups Ⅱ and Ⅲ were significantly higher than that of group Ⅰ (P<0.05);But the spleen index were not significantly different among the three groups (P>0.05).③ At 21 days of age,the content of IgA and IgM in serum of groups Ⅲ were significantly higher than that of group Ⅰ (P<0.05),and the content of IgG in serum of groups Ⅱ and Ⅲ were significantly higher than that of group Ⅰ (P<0.05).④At 21 days of age,the content of IL-2,TNF-α and sIgA in intestinal mucosa of groups Ⅱ and Ⅲ were significantly higher than that of group Ⅰ (P<0.05),and the content of IL-6 in intestinal mucosa of group Ⅲ were significantly higher than that of groups Ⅰ and Ⅱ (P<0.05).⑤At 21 days of age,the villus height of jejunum in groups Ⅱ and Ⅲ were significantly higher than that of group Ⅰ (P<0.05),the villus height/crypt depth of jejunum in group Ⅱ was significantly higher than that of groups Ⅰ and Ⅲ (P<0.05). However,the crypt depth was not significantly different among the three groups (P>0.05).In conclusion,that Fagopyrum dibotrys (D.Don) Hara could not affect the growth performance of broilers,but it could improve the development of immune organs,increase the secretion of serum immunoglobulin, promote the immune function of intestinal mucosa and intestinal morphology of jejunum.     

里氏木霉重组t-PA纯度、分子量及等电点鉴定

基因工程菌株里氏木霉(Trichodermareesei)生物合成的组织型纤溶酶原激活剂(tissue-typeplasminogenactivator,t-PA)经分离纯化后,采用还原SDS-PAGE和非还原PAGE分析达到了电泳纯,采用Superdex75PrepGrade凝胶过滤色谱分析达到了色谱纯;采用还原SDS-PAGE分析其相对分子质量为6.6×104,SDS-聚丙烯酰胺凝胶电泳纤维蛋白自显影法检测其相对分子质量为6.6×104,与相关文献报道的一致。等电聚焦电泳测定其等电点为4.24。     

高龄大熊猫多器官衰竭的病理学观察

本文观察了1只38岁高龄的大熊猫多器官衰竭的病理学变化。病理剖检显示，该熊猫主要表现为实质器官充血、水肿和出血性变化；组织病理学检查发现，全身实质器官（除心、肺、脑外）均发生明显萎缩，胃、肠、肝、胰、肾等发生水肿变性或出血；此外，还观察到心肌纤维坏死后的瘢痕形成。结果表明，老龄大熊猫的多器官衰竭的病理学变化与其他动物相似；高龄大熊猫也易患心血管系统疾病，且具有较经的组织修复能力。