A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide

The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide.     

干法膨化宠物饲料技术参数关系的研究

采用双螺杆膨化机干法工艺对宠物全价粉状饲料进行膨化并制粒,研究所得产品的容重与淀粉糊化度、硬度指标之间的关系,以及设备参数和配方特性对颗粒饲料容重的影响。结果表明:容重与淀粉糊化度、硬度密切相关;设备参数与配方特性均对产品容重有极显著影响;通过容重的测定估测其熟度和硬度具有可行性;可以通过调节喂料速度和膨化玉米比例控制产品容重。     

Effect of dietary nutmeg oil on heat‐stress tolerance‐related parameters in Korean native chicken reared under hot temperature

This study investigated the effect of dietary nutmeg oil (NO) on growth performance, blood parameters, lipid peroxidation and heat shock protein (HSP) 70 expression in Korean native chicken (KNC) reared under hot temperature. We allocated 273 meat‐type KNCs (Hanhyup‐3, 4‐week‐old, body weight [BW] = 539.93 ± 1.75 g) to the following three treatments with seven replicate pens (13 birds/pen) per treatment. Three treatment diets were as follows: (a) Control, basal diet without NO supplementation; (b) NO 250; and (c) NO 500, basal diet supplemented with 250 and 500 ppm NO respectively. Diets and water were provided ad libitum throughout the 6‐week feeding trial. During overall period (0–6 weeks), no differences (p > 0.05) were observed in BW gain (BWG), feed intake (FI) and feed conversion rate (FCR) among treatments. However, the FI at 0–3 weeks decreased (p < 0.05) quadratically with increasing NO levels. Most blood parameters did not differ (p > 0.05) among treatments, although the monocyte level of the NO 500 group was considerably lower (p > 0.05) than that of the other groups. Furthermore, dietary NO did not affect serum triglyceride, cholesterol, total protein, albumin, calcium, phosphorus and alanine aminotransferase (ALT) levels (p > 0.05); however, it linearly decreased serum aspartate aminotransferase (AST) level (p < 0.05). Additionally, serum malondialdehyde (MDA) concentration decreased (p < 0.05) and heart MDA concentration was lower (p = 0.08) with increasing dietary NO supplementation. After a 3‐hr heat (35°C) challenge, the rectal temperature (RT) reduced (p < 0.05) linearly with increasing NO levels. Dietary NO did not affect liver HSP70 (p > 0.05) gene expression. In conclusion, NO potentially enhanced the ability of chickens to alleviate heat stress. Furthermore, our findings suggest that lipid oxidation inhibition by dietary NO likely mediated the enhanced heat‐stress tolerance of the chickens.     

野生植物白沙蒿资源的综合开发利用研究

对白沙蒿的生物学特性、化学成分及开发应用进行了综合研究，结果表明：白沙蒿是理想的抗旱、防沙和固沙植物；含有丰富的营养物质，可作为畜牧业的重要饲料；有较高的药用价值和工业利用前景，对该植物资源的开发利用提供了科学依据。     

草地农业生态系统的自组织特性

根据自组织理论探讨了草地农业生态系统的6个自组织特性，即草地农业生态系统的结构性、有序性、非线性、开放性、非平衡性和涨落性。认为，草地农业生态系统是一个自组织系统；它只有不断地与外界进行物质、能量和信息的交换及系统内部的协同效应，才能产生结构和功能的有序状态；对草地农业生态系统的调控，必须抓住物质和能量输入的控制，特别是要控制系统内部生态的、经济的、技术的因素以及生产劳动因素之是的协同效应。     

从甘肃奶业发展趋势看草业发展的前景

本文从甘肃的特点论述了“尽快把畜牧业发展成一个大产业”，抓住以退耕还林还草为重点的西部大开发机遇，大力发展草业，加快实现种植业“二元结构”向“三元结构”的转变。大力发展奶业，草业和奶业的结合是历史的必然。     

DA-6对野大麦幼苗光合作用和生长的影响

研究了不同浓度的植物生长促进剂--DA-6对野大麦Hordeum brevisubulatum幼苗光合作用和生长的影响.在二叶期喷施较低质量浓度(5 mg/L,10 mg/L)的DA-6,可显著促进幼苗生长和叶绿素含量的提高;四叶期喷施较高质量浓度(20 mg/L,30 mg/L, 40 mg/L)的DA-6,可显著提高野大麦幼苗的生物量,其中20 mg/L和30 mg/L的处理,幼苗生物量分别提高55.1%和59.6%;光合作用能力分别提高12.8%和18.1%.     

新城疫（ND）—减蛋综合征（EDS76）蜂胶二联灭活疫苗的研制Ⅰ．制苗工艺、疫苗安全性和免疫原性试验

将新城疫病毒（NDV）Colon-30株和减蛋综合征病毒（EDS76AV）－127株分别接种于非免疫鸡胚和鸭胚，制备抗原液。抗原液经甲醛灭活后，与纯化的天然免疫增强剂蜂胶乳化，制成鸡新城疫（ND）－减蛋综合征（EDS76）蜂胶二联灭活疫苗，对疫苗的安全性和免疫原性进行了测定。对3－6周龄易感雏鸡以2倍使用剂量肌内注射，安全性良好；对1日龄雏鸡接种1个使用剂量进行急性毒性试验。15d生长曲线表明，对雏难 影响。对6周龄雏鸡注射1个使用剂量，每批疫苗免疫10只鸡，免疫后5－32d，鸡血清中EDS76血凝抑制（HI）抗体的几何平均滴度（GMT）为6．5－11．8log2,ND血凝抑制（HI）抗体的几何平均滴度（GMT）为4．8－9．8log2；对ND免疫攻毒试验表明，3批疫苗，每羽份含有的半数保护量（PD50）分别为≥127、≥131和≥127。     

哺乳动物卵母细胞发育机理的研究进展

卵母细胞的成熟过程是一个复杂的减数分裂过程，许多分子参与了这个过程的调控，本文就卵母细胞成熟抑制因子（OMI）、成熟促进因子（MPF）、生长分化因子（GDF）、微管辅助蛋白（MAP）、激素、Ca^2 及钙调素等对卵母细胞发育调控机理作一综述。     

2020—2021年湖南省娄底市活禽批发市场市场链调查

为了解湖南省娄底市活禽批发市场活禽来源与流通情况,对2020—2021年娄底市2个活禽批发市场活禽调入、调出情况进行了调查.结果显示:每年调入娄底市活禽批发市场的活禽超过3000批次,数量超过600万羽;调入活禽种类有鸡、鸭、鹅、鸽等品种,其中活鸡比例超过80％;活禽批发市场主要以省内调入为主,还涉及12个省(自治区)...