壳聚糖对动物脂肪代谢及生长性能的影响

壳聚糖(chifosan),是甲壳素的衍生物．又名可溶性甲壳素、脱乙酰甲壳素、甲壳胺、几丁聚糖．是通过甲壳素一定程度的脱乙酰而得到．通常将脱乙酰度55％以上的甲壳素称作壳聚糖,化学名称是(1．4)-2-氨基-2-脱氧-β-D-葡聚糖。在众多的天然多糖中,甲壳素及其主要衍生物壳聚糖是唯一的碱性多糖．具有生物相容性、可食用性、可抗菌性、     

Synthesis and Characterization of 2-Decenoic Acid Modified Chitosan for Infection Prevention and Tissue Engineering

Chitosan nanofiber membranes are recognized as functional antimicrobial materials, as they can effectively provide a barrier that guides tissue growth and supports healing. Methods to stabilize nanofibers in aqueous solutions include acylation with fatty acids. Modification with fatty acids that also have antimicrobial and biofilm-resistant properties may be particularly beneficial in tissue regeneration applications. This study investigated the ability to customize the fatty acid attachment by acyl chlorides to include antimicrobial 2-decenoic acid. Synthesis of 2-decenoyl chloride was followed by acylation of electrospun chitosan membranes in pyridine. Physicochemical properties were characterized through scanning electron microscopy, FTIR, contact angle, and thermogravimetric analysis. The ability of membranes to resist biofilm formation by S. aureus and P. aeruginosa was evaluated by direct inoculation. Cytocompatibility was evaluated by adding membranes to cultures of NIH3T3 fibroblast cells. Acylation with chlorides stabilized nanofibers in aqueous media without significant swelling of fibers and increased hydrophobicity of the membranes. Acyl-modified membranes reduced both S. aureus and P. aeruginosa bacterial biofilm formation on membrane while also supporting fibroblast growth. Acylated chitosan membranes may be useful as wound dressings, guided regeneration scaffolds, local drug delivery, or filtration.     

Biomass management targets and the conservation and economic benefits of marine reserves

The establishment of no‐take marine reserves has been increasingly promoted as a key measure to achieve conservation and sustainability goals in fisheries. Regardless of the wide range of benefits cited, the effectiveness of reserve establishment depends critically on fisheries management outside the reserves. We construct a bioeconomic model of a fishery that allows for the establishment of a no‐take marine reserve and evaluate how the choice of the off‐reserve management target influences the effectiveness of reserve establishment. We evaluate two biomass targets: (i) B_MSY or the biomass that produces the maximum sustainable yield (MSY) and (ii) B_MEY or the biomass that maximizes the net present value of the returns to fishing. The parameterized model shows that, for a wide range of scenarios, the fishery will be better off in terms of both conservation and economic objectives when the no‐take reserve is established in conjunction with the B_MEY target rather than with the B_MSY target. Model results further show that the opportunity cost of securing additional fish biomass, in both deterministic and stochastic environments, is lower when the reserve size is increased under the B_MEY target. This finding is important because marine reserves have been established as a key measure to restore depleted fish stocks, and the results suggest that this objective can be achieved with lower economic costs in a B_MEY managed fishery.     

Trawl fishing impacts on the status of seabed fauna in diverse regions of the globe

Bottom trawl fishing is a controversial activity. It yields about a quarter of the world's wild seafood, but also has impacts on the marine environment. Recent advances have quantified and improved understanding of large‐scale impacts of trawling on the seabed. However, such information needs to be coupled with distributions of benthic invertebrates (benthos) to assess whether these populations are being sustained under current trawling regimes. This study collated data from 13 diverse regions of the globe spanning four continents. Within each region, we combined trawl intensity distributions and predicted abundance distributions of benthos groups with impact and recovery parameters for taxonomic classes in a risk assessment model to estimate benthos status. The exposure of 220 predicted benthos‐group distributions to trawling intensity (as swept area ratio) ranged between 0% and 210% (mean = 37%) of abundance. However, benthos status, an indicator of the depleted abundance under chronic trawling pressure as a proportion of untrawled state, ranged between 0.86 and 1 (mean = 0.99), with 78% of benthos groups > 0.95. Mean benthos status was lowest in regions of Europe and Africa, and for taxonomic classes Bivalvia and Gastropoda. Our results demonstrate that while spatial overlap studies can help infer general patterns of potential risk, actual risks cannot be evaluated without using an assessment model that incorporates trawl impact and recovery metrics. These quantitative outputs are essential for sustainability assessments, and together with reference points and thresholds, can help managers ensure use of the marine environment is sustainable under the ecosystem approach to management.     

内蒙古草原污染源及对畜体危害分析

随着内蒙古草原牧区工农业、旅游业的发展,草原生态受到了一定影响。工业及生活污染日趋严重,造成放牧场牧草污染,污染残留物的转移和积蓄也影响了家畜正常健康的生长。简单概述了草原牧草的污染源、污染元素及对畜体危害。     

内蒙古苏尼特右旗草原土壤营养元素有效态含量分析

本研究选择内蒙古苏尼特右旗天然草场主要6个草场类,8个草场型,分析了0~30 cm土壤营养元素(全N、P、K、Ca、Mg、S、Fe、Mn、Cu、Zn、B和Mo)有效态含量及与土壤有机质、pH值之间的相互关系。结果表明,1)研究区土壤pH(8.84±0.23),均为碱性土壤;土壤有机质含量与全N含量均较低,平均分别为(9.92±4.65) g/kg和(0.46±0.25) g/kg;营养元素有效态含量积累顺序为:河泛地、湖盆低地盐生草甸草场类>荒漠草原草场区“小针茅+无芒隐子草+葱属”植被型>沙丘植被草场类,河泛地、湖盆低地盐生草甸草场类土壤矿质元素有效态含量显著高于其他草场类。2)土壤富含K元素,B和Mo处于严重缺乏状态;除了河泛地、湖盆低地盐生草甸草场类以外草场类微量元素含量均处于轻度缺乏或缺乏状态。3)土壤pH与土壤N、P、Ca、Mn和Cu元素有效量存在显著负相关性关系;土壤有机质则与土壤N、P、Ca、S、Fe、Mn和Cu元素有效量存在极显著正相关性关系。     

A leaf-blotch disorder of tayberry associated with the leaf and bud mite (Phyllocoptes gracilis) and some effects of three aphid-borne viruses

Tayberry plants showing conspicuous chlorotic blotches, ringspots, leaf distortion and lacking vigour were found at two sites in Scotland. The severity of the symptoms and the number of plants affected increased annually and reached 100% incidence at one site. No mechanically transmissible virus was detected in affected plants, but many such plants and some symptomless ones contained viruses indistinguishable in Rubus indicator plants from black raspberry necrosis, raspberry leaf mottle and raspberry leaf spot viruses. Propagants from healthy tayberry plants graft-inoculated either with these three viruses, singly and in combinations, or with scions from symptom-bearing tayberry, showed a faint chlorotic mottle when grown outdoors or in an unheated glasshouse but none developed the severe symptoms observed in the field. Leaves and buds of field-affected plants were found to be infested with large numbers of eriophyid mites at both sites. A single application of vamidothion to affected plants at one site resulted in significantly fewer mites and less severe symptoms on fruiting canes and primocanes than on unsprayed plants in the year of spraying and a further decrease in symptoms in the following year. The symptomatology, occurrence in Scotland and the decrease in symptoms following the application of vamidothion indicate that the disorder is caused by infestations by the leaf and bud mite (Phyllocoptes gracilis).     

Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme‐Linked Immunosorbent Assay for PSPB and the Progesterone Assay

This study was undertaken to detect pregnancy in Iraqi riverine buffalo (Bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy‐specific protein B (PSPB) with the BioPRYN^® enzyme‐linked immunosorbent assay (ELISA) test. The aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. Twenty‐two female riverine buffalo that were 6.0 ± 0.93 years old were used. Four blood samples per buffalo were taken via jugular venipuncture at days 22–24, 32–34, 42–44 and 58–61 post‐mating (PM) to measure the progesterone concentration (ng/ml) and to detect the presence of plasma PSPB. The rectal palpation method was employed to evaluate all buffalo on days 42–44 and 58–61 PM. The BioPRYN^® test differed (p < 0.01) from the other tests with earlier accuracy for detecting pregnant and non‐pregnant buffalo. Eighty‐eight percent of pregnant and 76.9% of non‐pregnant buffalo were distinguished early (days 22–24 PM) using BioPRYN^® and plasma PSPB‐ELISA level (2.09 ± 0.12 ng/ml) in relation to 66.7% and 53.9% detected using the progesterone assay at similar days (4.30 ± 0.40 ng/ml). In conclusion, these results described, for the first time, the early and accurate pregnancy detection of water riverine buffalo using BioPRYN^® technology and provided the plasma levels of PSPB using an ELISA test. These findings will improve the reproductive and productive efficiency of Iraqi riverine buffalo by adapting the recent management and reproductive strategies in Iraq and in the world.