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31.
The commercial and economic value of genetically modified crops is determined by a predictable, consistent and stable transmission
and expression of the transgenes in successive generations. No gene inactivation is expected after selfings or crosses with
non-transformed plants of homozygous transgenic oilseed rape plants if the expression of the transgene in homozygous or hemizygous
nature in such plants is stable. The segregation ratios of phosphinothricin (PPT) tolerance in successive generations of selfings
and mutual crosses of a few independent transgenic PPT-tolerant oilseed rape plants indicated a dominant, monogenic inheritance.
In within-variety and between-variety crosses no transgene inactivation was observed. However, after selfings and backcrosses
with non-transgenic oilseed rape infrequent loss of the expression of the PPT tolerance transgene was observed independent
from its homozygous or hemizygous nature. Molecular analysis of PPT-susceptible plants showed that the loss of expression
was due to gene inactivation and not to the absence of the transgene. Methylation and co-suppression are mechanisms that might
cause reduced or even loss of expression of the transgene in later generations. The implications of this observation for seed
multiplication of varieties and breeding activities with transgenic oilseed rape are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
32.
Colchicine induced polyploids have not directly contributed for crop improvement in the past. On the other hand, the so-called
natural polyploids, derived from the functioning of numerically unreduced(2n) gametes have been shown to be more relevant for crop improvement in many cases. Different types of cytological abnormalities
during meiosis can give rise to 2n gametes and the genetic composition of these gametes is variable. Depending on the type meiotic abnormalities, various types
of 2ngametes, such as first division restitution(FDR), second division restitution (SDR),indeterminate meiotic restitution (IMR)
and post meiotic restitution (PMR) gametes,among others, have been described in recent years. For the improvement of autopolyploids
such as potato, alfalfa,Vaccinium spp., and some of the fodder grasses, FDR gametes have been proved to be highly useful. However, the use of 2n gametes for the improvement of allopolyploid crops has received much less attention so far. Some of the investigations on
allopolyploids, derived from Festuca-Lolium, Alstroemeria and Lilium species hybrids, have revealed that 2ngametes can be most useful for the introgression of alien genes and chromosomes into cultivars. An important feature of using
sexual polyploidization in the case of allopolyploids is that introgression can be achieved through recombination due to genetic
crossing-over between alien chromosomes as well as addition of alien chromosomes, which is extremely difficult or impossible
to achieve in the case of colchicine induced allopolyploids. Because of the recent developments in the field of plant molecular
biology, methods have become available for the analysis of 2ngametes and sexual polyploid progenies more accurately and to develop systematic breeding approaches. The methods include
DNA in situ hybridization (GISH and FISH)and molecular mapping (AFLP, RFLP, RAPDs).In addition to providing basic information
on the genetic and genome composition of the polyploid progenies, these methods can be potentially useful for a more efficient
creation of desirable breeding material and cultivars.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
33.
R. C. B. Hutten M. G. M. Schippers J. Eising P. M. van Til J. G. Th. Hermsen E. Jacobsen 《Euphytica》1996,88(3):175-179
Summary The parental effects on progenies from potato 4x.2x(FDR) crosses were analyzed to determine anether and to what extent selection criteria used for selecting tetraploid breeding lines have to be adjusted when selecting diploid breeding lines. For vine maturity and chip colour multiple regression analysis of progeny means on means of diploid and tetraploid parents revealed high coefficients of determination (R2) for both characters, indicating good predicting power of the performance of both diploid and tetraploid parents on the performance of their 4x.2x progenies. For vine maturity the multiple regression slope for the tetraploid perents was significantly larger than for the diploid parents, and progeny means were towards the tetraploid parents. This indicates that selection criteria for vine maturity may be less stringent at the diploid level. However, diploids may be earlier maturing than tetraploids and therefore selection criteria can better be similar at both ploidy levels. For chip colour, multiple regression slopes for diploid and tetraploid parents were similar and progeny means were towards the darkest coloured parent irrespective of its ploidy level. This indicates that selection of breeding lines for chip colour can be conducted similarly at both ploidy levels. 相似文献
34.
Summary Selection criteria for agronomic characters in a potato breeding program at the diploid (2x) level may differ from selection criteria used when selecting breeding lines at the tetraploid (4x) level. Differences between selection criteria are expected, (1) when expression of the characters is different at both ploidy levels and/or (2) when the effect of diploid breeding lines on agronomic characters of tetraploid progenies is different from the effect of tetraploid breeding lines. In this investigation sets of diploid and tetraploid progenies, each set derived from the same 2x.2x cross, were compared as to the expression of six agronomic characters. Diploid progenies had significantly lower yields (due to smaller tubers) and significantly higher under water weights than tetraploid progenies. Vine maturity and chip colour were similarly expressed at both ploidy levels. Correlations between yield and yield components, and between under water weight and chip colour were similar at both ploidy levels. The lower yields and higher under water weights found in diploids point to the need of different selection criteria for selecting diploid and tetraploid breeding lines. 相似文献
35.
For the selection of diploid (2n = 2x = 24) potato (Solanum tuberosum) genotypes that are useful for the molecular and genetic
analysis of the phenomenon of gametophytic self-incompatibility, three different types of basic populations were investigated.
These populations were derived from three primary dihaploid clones, G609, G254 and B16, which possessed the S-allele combinations
S1S2, S1S3 and S3S4 respectively. In order to select highly vigorous, profusely flowering, fertile and tuberising progenies,
three types of populations, derived from the above mentioned diploid genotypes, were screened for performance and classified
for the expression of self-incompatibility. Although the selection for well defined S-genotypes was sometimes complicated
due to the occurrence of pseudo-compatibility and of a self-compatibilising factor, the use of a combination of criteria,
viz., Iso Electric Focusing (IEF), pollen tube growth in the styles and the extent of berry and seed set made the selection
of sufficient representatives of all six types of S-heterozygotes (S1S2, S1S3, S1S4, S2S3, S2S4 and S3S4) possible. After
evaluating the strength of the self-incompatibility reaction in these heterozygotes, those with high expression were selfed,
and intercrossed within their S-allele incompatibility group through the method of counterfeit pollination. In these progenies,
well-performing S-homozygotes (S1S1; S2S2; S3S3; S4S4) for all four S-alleles with high expression of self-incompatibility
were selected. As a result, all possible S-homo- and heterozygous genotypes with a predictable type of self-incompatibility
are available and maintained both vegetatively and as botanical seed. The development of this material has paved the way for
more critical analysis of molecular factors involved in self-incompatibility in diploid potato.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
36.
A comparison of progeny from diallel crosses of diploid potato with regard to the frequencies of 2n-pollen grains 总被引:1,自引:0,他引:1
Summary Selection of diploid potato genotypes that produce high frequencies of 2n-pollen grains in different environments is an important requirement for analytic breeding as well as for true potato seed production. In this context, quantitative estimates of 2n-pollen formation were made in two type of diploid potato populations. One population set consisted of the progeny from diallel crosses of five well-known diploid potato clones (A=US-W 5293.3, B=US-W 5295.7, C=US-W 5337.3, D=US-W 7589.2 and E=772102-37). Another population set was obtained by pollinating three genotypes of Solanum phureja, IvP35, IvP48 and IvP101 with clones A, C and E. It was observed that the behaviour of the parental clones with respect to 2n-pollen grains formation in different environments, such as in Europe and China, was stable. The correlation coefficient between means of 2n-pollen grains frequency of combinations and percentage of genotypes with more than 5% 2n-pollen grains in a given combination were estimated and that between mean and maximum frequency of 2n-pollen grains formation in each combination. The results showed that the former coefficients were significant wheareas the latter were not. The combining ability analysis revealed variation in 2n-pollen grains formation between families. Specific combining ability (SCA) effects contributed much more than general combining ability (GCA) to the variation in 2n-pollen grains formation. The broad-sense heritability of 2n-pollen grains formation was estimated to vary between 0.45 and 0.53 and the narrow-sense heritability was calculated to be 0.15 and 0.20 in the two types of populations. This means that additive variance was relatively low. Two to four genes were estimated to control the inheritance of 2n-pollen grains formation. 相似文献
37.
Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato 总被引:2,自引:0,他引:2
G. J. Kuipers J. T. M. Vreem H. Meyer E. Jacobsen W. J. Feenstra R. G. F. Visser 《Euphytica》1991,59(1):83-91
Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content. 相似文献
38.
R. C. B. Hutten E. J. M. M. Scholberg D. J. Huigen J. G. Th. Hermsen E. Jacobsen 《Euphytica》1993,72(1-2):61-64
Summary The production of dihaploids is the first step in a potato breeding program at the diploid level. Dihaploid induction ability, dihaploid production ability and seed parent x pollinator interaction were analyzed for 28 × 3, seed parent x pollinator combinations. This is the first report on significant interaction between seed parents and pollinators. Despite this interaction, IVP 101 had a significantly higher dihaploid induction ability than the widely used pollinators IVP 35 and IVP 48. Previous findings on significant differences in dihaploid production ability between seed parents were clearly confirmed. 相似文献
39.
Anne-marie Wolters Evert Jacobsen Mary O'Connell Guusie Bonnema K. Sree Ramulu Hans de Jong Herman Schoenmakers Jelle Wijbrandi Maarten Koornneef 《Euphytica》1994,79(3):265-277
Protoplast fusion can be used to produce somatic hybrids of species that cannot be obtained by sexual hybridization. The possibility to introgress genes from Solanum species into the cultivated tomato species Lycopersicon esculentum, and to obtain novel cytoplasm-nucleus combinations (cybrids) was considered as an important strategy to extend the genetic variation available for tomato breeding. Somatic hybrids between L. esculentum and other Lycopersicon species, as well as between L. esculentum and Solanum or Nicotiana species, have been produced. Specific mutants, genotypes with antibiotic resistances, and metabolic inhibition by iodoacetate or iodoacetamide and irradiation were used for the selection of hybrids. In addition, the improvement of protoplast culture techniques and the use of the favourable tissue culture traits derived from species such as L. peruvianum, which have been introduced into tomato by classical breeding, allowed the efficient recovery of somatic hybrids. However, the occurrence of somatic incongruity in fusion combinations of L. esculentum and Solanum and even more in L. esculentum and Nicotiana, did not allow the production of true cybrids and/or fertile hybrids, indicating the importance of both cytoplasm-nucleus and nucleus-nucleus interactions in somatic incongruity. Another problem with fusions between distantly related species is the strongly reduced fertility of the hybrids and the very limited homoeologous recombination between chromosomes of the parental species. Partial genome transfer from donor to recipient through microprotoplast (+) protoplast fusion, and the production of monosomic or disomic chromosome addition lines, light overcome some of these problems. In symmetric somatic hybrids between L. esculentum and S. tuberosum the occurrence of limited somatic and meiotic recombination was demonstrated. Fertile progeny plants could be obtained, though at a low frequency, when embryo rescue was performed on a large scale after backcrossing hexaploid somatic tomato (+) potato hybrids with a tetraploid potato genotype. The potential value of genomic in situ hybridization (GISH) and RFLPs for the analysis of the genome/chromosome composition of the hybrids has been demonstrated for intergeneric somatic hybrids between Lycopersicon and Solanum.Abbreviations cpDNA
chloroplast DNA
- mtDNA
mitochondrial DNA 相似文献
40.
Adaptation of quinoa (Chenopodium quinoa) to Northern European agriculture: studies on developmental pattern 总被引:1,自引:0,他引:1
Sven-Erik Jacobsen 《Euphytica》1997,93(1):41-48
The inheritance of resistance to clubroot, caused by Plasmodiophora brassicae in Brassica oleracea was studied in the F1, F2and backcross progenies of three crosses between resistant and susceptible doubled haploid lines. Fresh weights of affected (CW) and healthy (HRW) parts of the root system were measured and R = ln((HRW+0.5)/(CW+0.5)) was used as a measure of resistance. R was shown to have a normally distributed error term with a constant standard deviation over the entire scale. Analysis of means indicated the presence of two complementary resistance genes in one of the crosses. In the other two crosses analysis of means also indicated two-locus interactions, in these cases reducing the cumulative effects of loci homozygous for resistance or for susceptibility alleles. The numbers of segregating resistance genes in these two crosses could not be determined. The presence of epistatic interactions precluded analysis of variance; therefore in this case the measurements were not more informative than the symptom grades analyzed in a previous study.R showed a high rank correlation (-0.85) with symptom grades assigned to the same plants. The results of genetic analyses of R were generally in agreement with those obtained earlier using symptom grades. Symptom grades can therefore be regarded as a reliable measure of symptom development, despite their more subjective nature. 相似文献