千里光提取物冻干粉药代动力学研究-抗炎药理效应法

为了探索千里光提取物冻干粉(SsE)的药物代谢动力学特征,首次采用耳肿胀度抑制率药理效应法测定SsE药动学参数。结果发现,在一定剂量范围内给小鼠腹腔注射SsE,能较迅速地产生药理效应,使耳肿胀度抑制率显著提高;SsE最低有效量为57.40 mg/kg,在小鼠体内代谢符合一级反应一室模型,模型表达式为:C=1 436.227 e^(-0.133 4 t)-1436.227 e^(-0.237 t),表观药动学参数为:一级消除速率常数Ke=0.133 4 h-1,消除半衰期t1/2Ke=5.194 9 h,一级吸收速率常数Ka=0.237 h-1,吸收半衰期t1/2Ka=2.924 1 h,血药峰浓度Cmax=1 436.227 mg/kg,达峰时间tmax=5.547 4 h,清除率Cl=0.055 3mg/kg.h,药-时曲线下面积AUC=16 826.35 mg/kg.h,表观分布容积V=0.414 2 mg/kg,滞后期t0=0.010 4 h。表明SsE具有良好的抗炎作用,在小鼠体内起效快,消除慢,生物利用度高,在机体内分布有限,较集中于血浆,组织摄入少。     

伪狂犬病病毒gB蛋白抗原表位基因串联构建及原核表达

本试验通过自行设计两段引物,利用重叠延伸PCR方法,将伪狂犬病病毒(PRV)gB基因两段优势抗原表位序列串联,克隆至pMD18-T载体,测序正确后双酶切连接至pET-32a(+)载体,构建重组质粒;重组质粒转化入大肠杆菌BL21(DE3)受体菌,经IPTG诱导后,通过SDS-PAGE和Western blotting检测融合蛋白表达情况。经检测,诱导后的重组蛋白获得表达,重组蛋白大小约为54ku,其中串联蛋白大小约为34ku。该重组蛋白可与伪狂犬病病毒gB蛋白单克隆抗体发生特异性反应,表明重组蛋白的抗原性良好。     

B亚型禽偏肺病毒逆转录套式PCR检测方法的建立与应用

根据GenBank发布的B亚型禽偏肺病毒Fusion(F)基因的保守序列设计2对引物,建立了一种适用于B亚型禽偏肺病毒的逆转录套式PCR检测方法.采用该方法对B亚型禽偏肺病毒山东分离株进行检测,可以特异性地扩增出415 bp的目的片段.此方法具有高度特异性和敏感性,以H9亚型禽流感病毒、新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒作为模板进行扩增,结果均为阴性.经检测,该方法第1次PCR扩增的敏感性为102 copies/μL,第2次扩增的敏感性为102 copies/μL,第2次扩增敏感性比第1次高105倍.应用本方法对采自山东省不同地区的64份可疑临床病料进行检测,最终从64份疑似病料中检测出37份为阳性.结果表明,所建立的套式PCR检测方法具有特异、敏感、实用等优点,为B亚型禽偏肺病毒的快速诊断以及深入研究奠定了基础.     

Src基因研究进展

Src是非受体蛋白酪氨酸激酶家族成员之一,属原癌基因,Src表达及活性异常往往引起乳腺癌、结肠癌、胰腺癌、前列腺癌、肺癌等某些肿瘤发生、发展.最近研究表明Src基因与乳腺的提前退化及泌乳失败相关.文从Src基因的定位、蛋白质结构、分布及功能等几方面进行简要的介绍.     

高通量测序技术结合传统方法筛选及鉴定奶酒中曲霉菌

奶酒含有丰富的营养,且具有驱寒活血、开胃消食等保健功效。但由于传统的可培养方法对其微生物全貌的认识具有一定的局限性。因此,本文采用高通量测序技术,对奶酒的真菌生物多样性进行检测。结果表明,酵母菌属(Saccharomyces)和曲霉属(Aspergillus)是奶酒最主要的真菌群落,其中,酵母菌属为第一丰度的真菌,丰度约为62%。第二丰度的真菌为曲霉属,丰度为33%。通过进一步分离并采用16S鉴定出了一株曲霉菌,这可为奶酒发酵剂的开发提供依据。     

传染性法氏囊病超强毒Gx株VP3基因在病毒致弱过程中的变异研究

利用SPF鸡胚对鸡传染性法氏囊病超强毒vvIBDV Gx株进行培育，通过鸡胚成纤维细胞对病毒进行传代致弱，对其结构蛋白VP3基因核苷酸及推导的氨基酸序列进行分析，从而揭示vvIBDV Gx从超强毒力向弱毒力转化过程中基因的序列变化规律。对不同代次细胞毒序列分析的结果表明，细胞毒在第8代以前，VP3基因序列没有改变，与标准超强毒HK46株氨基酸同源性达99％以上；细胞毒第9代核苷酸和氨基酸序列发生了改变；10代毒VP3基因与标准弱毒P2株氨基酸序列同源性达100％；细胞适应毒传至20代，其VP3基因序列不再改变。从而说明，vvIBDV Gx株在致弱过程中，VP3基因也随之改变。     

广西县级林地变更调查试点技术方法

为探索广西县级林地年度变更调查技术方法,以鹿寨县为例,运用遥感自动检测技术、森林资源管理信息平台等,对地类及管理属性变化图斑进行变更,形成本期林地数据库和林地变化数据库。文章分析了林地变更调查的关键技术方法,指出了存在的问题,并提出相应的建议。     

Genetic Diversity Analysis of Cervus elaphus Using AMELY Gene in Y Chromosome

In order to determine the genetic diversity in Cervus elaphus using AMELY gene in Y chromosome,200 blood samples from Cervus elaphus yarkandensis,Cervus elaphus asiaticus,Cervus elaphus xanthopygus,Cervus elaphus songaricus and Cervus elaphus kansuensis populations were collected and AMELY genes were sequenced in this study.The haplotype diversity of Y chromosome was analyed,phylogenetic tree was built to explore the genetic diversity and the paternal origins about Cervus elaphus.The results showed that:Cervus elaphus yarkandensis had the most variation sites and the highest nucleotide diversity.The genetic distance between Cervus elaphus yarkandensis and other Cervus elaphus were far.6 haplotypes were identified in this study,named as A1,A2,A3,A4,A5 and A6,respectively.Cervu elaphus yarkandensis,Cervus elaphus asiaticus and Cervus elaphus kansuensis had separate haplotype.The NJ and ML phylogenetic trees showed that Cervus elaphus songaricus,Cervus elaphus asiaticus,Cervus elaphus xanthopygus and Cervus elaphus kansuensis clustered together which Cervus elaphus yarkandensis and Cervus elaphus kansuensis were form a department,separately.Cervus elaphus asiaticus,Cervus elaphus xanthopygus and Cervus elaphus yarkandensis clustered into one branches and there might be gene exchange among Cervus elaphus yarkandensis,Cervus elaphus kansuensis and other Cervus elaphus.     

Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines

The effects of the severity and timing of leaf removal(LR) on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season. High-performance liquid chromatography(HPLC) was used to analyze the amino acids profiles of grape berries and wines. The basal leaves were removed at three time points(40, 56 and 72 days after flowering, named LR40, LR56 and LR72, respectively) at two severity levels(one at which the first, third, and fifth basal leaves of each shoot were removed(50% level); and another at which the first six basal leaves were removed(100% level)). The results showed that leaf removal had little impact on total soluble solids(°Brix), titratable acidity, pH or berry weight. The LR72-50% treated grapes had higher berry weight, titratable acidity and °Brix than those of the other treatments. The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50% treated grapes(2 952.58 and 2 764.36 mg L~(-1), respectively); the lowest were detected in LR72-100% treated grapes(2 172.82 and 2 038.71 mg L~(-1), respectively). LR72-50% treatment significantly promoted the synthesis of aspartic acid, serine, arginine, alanine, aminobutyric acid and proline at both severity levels for grapes, the concentrations of all of these amino acids were increased relative to the control concentrations. The LR72-50%, LR40-100% and LR72-100% treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids, such as arginine, alanine and serine, than did the control wines. Of all the amino acids studied, glycine, tyrosine, cysteine, methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines. The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines.     

鸡公山自然保护区芳香植物资源调查研究

鸡公山自然保护区内芳香植物资源丰富,经初步调查,共计26科106种,其中裸子植物4科11种,被子植物22科95种。论述了鸡公山自然保护区芳香植物的种类、分布及精油提取部位,对其开发利用提出了建议。