水利工程社会经济效益评估方法研

水利工程作为国民经济的基础设施和基础产业，有着巨大的社会经济效益。本文分析了水利投资与社会经济发展的关系，并提出了基于乘数理论和比例关系的三种评估模型，最后以实例进行分析，验证了所提模型的可行性，为研究水利工程社会经济效益评估提供新方法。     

Changes in serum TGF β1 in type 2 diabetic patients

AIM: To explore the changes in serum TGF β₁ in type 2 diabetes mellitus. METHODS: Forty-five cases type 2 diabetes mellitus patients were divided into three groups according to urine albumim excretion rate(UAER): normoalbuminuria(NA)group and microalbuminuria(MA) group and macroalbuminuria group (Overt DN). Serum TGF β₁, fasting blood glucose(FBG), HbA_1c,BUN,Cr,Ccr,lipidemia were detected in all cases. RESULTS: Serum TGF β₁ in NA, MA and ODN groups was higher than that in control. Serum TGF β₁ was positive correlation with Cr(r=0.390,P＜0.05), LDL(r=0.503,P＜0.01), HbA_1c (r=0.676,P＜0.01), and UARE(r=0.777,P＜0.01). CONCLUSION: Type 2 diabetes mellitus have a higher serum TGF β₁ than controls, serum TGF β₁ was positive correlation with HbA_1c and injury of renal function.     

鸭疫里氏杆菌-大肠杆菌二联灭活疫苗的研制

从河北某肉鸭场的发病鸭中分离出鸭疫里氏杆菌（Riemerella.anatipestifer，RA）BJ-D-1株，对该菌株采用液体培养、固体培养及鸡坯培养等三种不同方法进行增菌培养，然后与大肠杆菌O1、O2、O78株及河北地方大肠杆菌流行株等的浓缩菌液以一定比例混合，制成油乳剂灭活苗。皮下免疫7日龄雏鸭0.5mL/只。在河北某发病肉鸭场及周围肉鸭场进行田间试验，效果良好。     

Changes in action potential and the conduction time of ventricular cells in subendocardium and subepicardium during ischemia and early reperfusion

AIM:To investigate the electrophysiological characteristics of ventricular myocyte both in subendocardium and in subepicardium during ischemia. METHODS:Using modified Ferrier's method of ischemic and reperfusion injury in isolated guinea pig right ventricular wall. RESULTS: The transmembranal electric activities in subendocardium and in subepicardium were all significantly abnormal, and it was more significant in subendocardium. CONCLUSION: The alteration degree of ventricular myocyte in subendocardium and in subepicardium during ischemia and early reperfusion was different, and this might be the electrophysiological basis of the vulnerability of the subendocardium.     

苦瓜平脐蠕孢叶斑病病原鉴定

This study was conducted to identify the pathogens causing a new leaf spot disease on bitter gourd. The diseased leaves were collected from bitter gourd plantations around Nanning, Guangxi province. The pathogen causing this disease was identified by pathogenicity, morphology, and combined gene sequences analysis on ITS, TEF and GPD. The morphological characteristics of the pathogen were similar to that of Bipolaris bicolor. The representative isolate was clustered into one clade in phylogenetic tree with B. bicolor, which indicated that the leaf spot of bitter gourd was caused by B. bicolor. This is the first report of a leaf spot disease caused by B. bicolor on bitter gourd.     

入侵害虫蔗扁蛾在中国的风险性分析

为确定入侵害虫蔗扁蛾是否应列入检疫性害虫进行控制,根据WTO的<卫生与植物卫生措施协定>(SPS)要求,对其进行风险性分析.依据国际植物检疫措施标准(ISPM)规定的有害生物风险性分析(PRA)程序,利用相关模型,对各项分析指标进行分析并赋值运算.各分析指标及运算值为:(1)国内分布情况,风险值为2;(2)潜在的危害性,风险值为1.6;(3)寄主植物的经济重要性,风险值为3;(4)传播扩散的可能性,风险值为1.89;(5)风险性管理难度,风险值为1.67.蔗扁蛾的综合风险性值为1.98,风险指数已达到检疫性害虫条件,应列入检疫性害虫名单,最后提出了相关风险性管理对策.     

36个马铃薯品种的SSR分析

为明确36个马铃薯品种在DNA分子水平上的遗传差异,利用SSR分子标记技术对其多态性进行了分析。试验从90对SSR引物中筛选出适宜于36个马铃薯品种基因组DNA扩增的引物10对,PCR扩增获得301个SSR条带,多态性条带百分率为71.1%。引物STACCA3和SSR111扩增的SSR指纹图谱差异明显,可作为36个马铃薯品种间鉴别的分子依据。36个马铃薯品种间的遗传距离(GD)变幅为0.33～0.85,平均为0.54,其中,大于GD平均值的品种有20个,占供试品种的55.56%。以GD值0.57为基准,将36个马铃薯品种划分成7类,从DNA分子水平揭示出各供试品种间的亲缘关系。     

钾肥施用量与施用方式对丹大17玉米品质的影响

采用二次回归旋转组合设计,以丹东地区主栽玉米品种丹大17为试材,研究钾肥施用量与施用方式对玉米品质的影响。结果表明:硫酸钾做基肥时施用量为183.33～211.00kg/hm2,做追肥时施用量为32.75～65.76kg/hm2,在此施肥水平下玉米籽粒的蛋白质含量增加0.99%,脂肪含量增加0.50%,淀粉含量增加5.06%。钾肥做基肥和追肥分别在播种和大喇叭口期施用,可明显改善玉米品质,各项品质指标达到最佳。     

十字花科植物花旗竿花药发育的细胞形态学观察

采用常规石蜡切片技术,观察研究十字花科植物花旗竿的花药发育过程。研究结果表明:花旗竿每朵花6枚雄蕊,每个花药4个花粉囊;小孢子在四分体中的排列为四面体型;成熟花粉粒近球形,属3-细胞型花粉粒并有3个萌发沟;花粉囊壁由4层细胞构成,即表皮(1层)、药室内壁(1层)、中层(1层～数层)和绒毡层(1层);绒毡层发育属分泌型。     

紫苏籽对鹌鹑肉中脂肪酸种类及含量的影响

【目的】研究紫苏籽对鹌鹑肉中ω-3不饱和脂肪酸富集的影响。【方法】将150羽1日龄鹌鹑随机分成5组,每组3个重复,每重复10羽,其中第1组为对照组,饲喂自配的基础日粮,第2～5组为试验组,分别饲喂在基础日粮中添加质量分数8%,10%,12%和15%紫苏籽（磨碎）组成的试验日粮,于35和50日龄取样,测定鹌鹑肉中的基本营养成分和各脂肪酸含量。【结果】在基础日粮中添加10%紫苏籽可显著提高35和50日龄鹌鹑肉的粗脂肪含量（P＜0.05）,添加12%紫苏籽可显著提高35日龄鹌鹑肉的粗脂肪和50日龄鹌鹑肉的粗灰分含量（P＜0.05）;添加不同量的紫苏籽均可显著提高35和50日龄鹌鹑肉的C18∶3含量及35日龄鹌鹑肉中多不饱和脂肪酸（PUFA）和ω-3 PUFA含量（P＜0.05）,显著降低35和50日龄鹌鹑肉的ω-6 PUFA/ω-3 PUFA值（P＜0.05）。【结论】早期投喂紫苏籽有利于PUFA和ω-3 PUFA的积累,紫苏籽的添加量以10%为宜。