Protective effects of ulinastatin on kidney injury in rabbits after cardiopulmonary resuscitation

AIM: To investigate the effect of ulinastatin (UTI) on inflammatory cell infiltration, expression of tumor necrosis factor-α (TNF-α) and concentration of oxidative stress product malondialdehyde (MDA) in the kidney medulla after cardiopulmonary resuscitation (CPR) in rabbits and to access its potential mechanisms in kidney injury after CPR.METHODS: Twenty-four male New Zealand adult rabbits were randomly divided into control group and UTI group after return of spontaneous circulation (ROSC) from 5 min ventricular fibrillation induced by alternating current. The UTI at the dose of 2.5×10⁴ U/kg was administered immediately after ROSC to the animals in UTI group, the same volume of saline were injected in control group. The urinary output was recorded and the serum concentration of BUN and creatinine were detected every 4 h after ROSC. The animals were sacrificed 24 h after ROSC and the kidney medullas were obtained to observe the degree of inflammatory cell infiltration, the expression of TNF-α and the concentration of MDA.RESULTS: Six rabbits in control group and 6 animals in UTI group survived to the end point of experiment. The urinary output was decreased gradually to the lowest 8 h-12 h after ROSC and then increased in both groups. The urinary output in UTI group was more than that in control group 8 h after ROSC (P<0.05). The serum concentrations of BUN and creatinine were significantly lower in UTI group than those in control group 4 h after ROSC (P<0.05). The myeloperoxidase-positive cells in control group were much higher than that in UTI group (P<0.05). The expression of TNF-α and concentration of MDA in the kidney medullas in UTI group were lower than those in control group (P<0.05, P<0.01).CONCLUSION: The standard dose of UTI (2.5×10⁴ U/kg) administered in rabbits suffered from ventricular fibrillation for 5 min may alleviate the degree of inflammatory cell infiltration, decrease the expression of TNF-α and reduce the concentration of MDA in kidney medulla. UTI has protective effects on the renal function after CPR.     

黑头噪鸦的活动区、种群密度和栖息地利用

黑头噪鸦(Perisoreus internigrans)是我国青藏高原高山针叶林的特有鸟类,种群数量稀少,一些基本生活史资料至今依然缺乏.我们于2001-2004年间对该物种在卓尼和九寨沟地区的种群状况和栖息地选择进行了研究.研究表明黑头噪鸦通常以平均3.8只个体构成的“群体”形式活动,平均家域大小为42.0 ha,...     

草酸处理对杧果采后果实AsA-GSH循环系统的影响

 杧果（Mangifera indica L.）栽培品种‘圣心’采前套袋果实采后经5 mmol · L^-1草酸溶液浸泡10 min后常温（25 ℃）下贮藏,期间果实硬度系数、病情指数、腐烂率、黄化都显著低于对照;其中,处理果实果皮的还原型抗坏血酸（AsA）和还原型谷胱甘肽（GSH）含量保持较高,抗坏血酸氧化酶（APX）和谷胱甘肽还原酶（GR）活性在贮藏9 d后提高,同时,超氧阴离子（）生成速率、过氧化氢（H₂O₂）含量和丙二醛（MDA）含量降低,说明草酸处理可通过提高果皮AsA-GSH循环系统中抗氧化酶活性和抗氧化剂水平来增强清除活性氧自由基（ROS）能力,减轻膜脂过氧化伤害,进而有利于延缓套袋杧果采后成熟衰老进程,提高果实的商品率。     

嗜热毛壳菌纤维素酶活力测定及稳定性研究

本试验用绵羊瘤胃液分别处理嗜热毛壳菌纤维素酶和对照酶,对其稳定性进行研究。试验结果表明,经绵羊瘤胃液处理1h和6h后,嗜热毛壳菌纤维素酶酶活力分别为原活力的88.41%和74.04%,而对照酶酶活力分别为原活力的58.30%和46.64%。从整体来说,嗜热毛壳菌纤维素酶在绵羊瘤胃中稳定性优于对照酶。     

平武黄牛血液蛋白质多态性研究

应用醋酸纤维薄膜和垂直板聚丙烯酰胺凝胶电泳技术对四川平武黄牛地方牛种的血红蛋白（Hb)和血清运铁蛋白（Tf)座位多态性进行了分析。结果：平武黄牛Hb座位含有3种基因HbA,HbB和HbC,HbC为优势基因，组成5种表现型，Tf座位有4个等位基因，TfD1和TfE为优势基因，组成10种表现型；与秦川牛，宣汉牛比较，Hb和Tf座位的不同基因及其因型频率都有很大差异。     

25种优良阔叶树种苗木生长节律研究

通过对吉安双江林场和南昌树木园3年来25个优良阔叶树种的育苗试验，充分表明了各树种育苗时在两地的物修及生长节律不尽相同，不同的树种在同一地点的生长节律有些树种的差异较大，有些树种的差异较小，对不同苗木的生长节律进行了初步的总结。     

新形势下农业高职毕业生就业问题探析

从一个新的视角,紧紧围绕“三农”与社会主义新农村建设问题,分析了目前农业高职毕业生面临的就业形势,针对目前农业高职毕业生的就业现状及存在的问题,提出了相应的解决对策。     

春油菜品种的产量及稳定性分析

用Eberhat-Russell法、关联度法、高稳系数法分析了1992年黑龙江垦区油菜5个参试品种(品系)的丰产稳产性,并对几种方法进行比较,结果表明关联度法是测定油菜品种丰产稳产性一个比较准确而简便的方法。     

北山林区云杉人工造林更新的调查

通过对近十年来北山林区云杉人工造林更新调查及成果分析，结果表明效果是明显的，并提出今后发展的建议措施。     

不同质量浓度二氧化氯对三种河蟹致病菌的杀灭效果

以1％的Na2S2O3为中和剂，测定了五种质量浓度100，20，4，0．8，0．16mg/L ClO2溶液对副溶血弧菌、毛霉亮发菌以及嗜水气单胞菌等河蟹致病菌的杀灭效果。同时以蛋白胨质量为材料测定了有机物对ClO2杀菌效果的影响。结果表明：0．8mg/L的ClO2作用15min、0．16mg/L的ClO2作用30min可有效杀灭三种致病菌；蛋白胨浓度为25mg/L时，对ClO2的杀菌效果几乎没有影响，蛋白胨质量浓度为25mg/L时，ClO2作用15min的杀菌率下降为97．5％，蛋白胨质量浓度为625mg/L时下降为95．5％。