28％苄嘧磺隆·二氯喹啉酸WP对水稻秧田杂草及产量的影响

研究了28％苄嘧磺隆·二氯喹啉酸对水稻秧田稗草[Echinochloa crusgalli (L.) Beauv.]、鸭舌草[Monochoia vaginalis(Burm.f.)]等的防除效果,并测定了杂草对氮、磷、钾和水分的影响以及对水稻产量的影响.结果表明,28％苄嘧磺隆·二氯喹啉酸WP对水稻秧田的主要杂草具有良好的防治效果,有效成分用量为168～420 g/hm2的28％苄嘧磺隆·二氯喹啉酸对水稻秧田稗草密度防效和鲜重防效分别为84.06％～98.50％、91.55％～98.63％,对鸭舌草的密度防效和鲜重防效分别为94.06％～100.00％、90.94％～100.00％,显著优于对照药剂10％苄嘧磺隆WP和50％二氯喹啉酸WP.28％苄嘧磺隆·二氯喹啉酸施用后,增产效果总体优于对照药剂,在水稻秧田具有良好的推广前景.     

杂交萝卜阳畦育苗半成株高产制种技术

杂交萝卜的采种方法主要有成株采种法、半成株采种法和小株采种法，目前生产上主要应用杂交一代种子，济源地区主要采用半成株采种法生产种子。现根据实验情况和多年实践经验对杂交萝卜阳畦育苗半成株高产制种技术做一简要介绍。     

豌豆蛋白质提取工艺的优化

以水为提取剂从豌豆粉中提取蛋白质,研究了pH值、温度、用水量和搅拌速度对豌豆蛋白质提取量的影响。通过正交试验,确定最佳提取工艺条件,即:pH值9、温度35℃、用水量每克豌豆粉40mL、搅拌速度240r/min。在该条件下豌豆蛋白质的提取量最高,蛋白质含量为201.04mg/g。     

信阳乡村旅游发展优势分析

作者对信阳发展乡村旅游的资源、区位、政策、市场及效益等方面的优势加以分析归纳,总结出信阳发展乡村旅游优势明显。信阳应利用自身优势,大力发展乡村旅游,振兴地方经济。     

Detection of HCV core protein,envelope glycoprotein and core-envelope fusion protein by baculovirus surface display

AIM: To detect the hepatitis C virus (HCV) at early stages by the technique of baculovirus surface display. METHODS: We constructed the recombinant baculoviruses vAc-Flag-HCV C-GP64, vAc-Flag-HCV E-GP64 and vAc-Flag-CE-GP64, which displayed HCV-related structural proteins by the technique of baculovirus surface display. RESULTS: Western blotting results showed that the core (C), envelope (E) and core-envelope (CE) proteins of HCV were expressed in Sf9 cells after the infection of recombinant baculoviruses. We also observed by confocal microscopy that the C, E and CE proteins of HCV presented and were anchored on the plasma membrane of Sf9 cells after infection. In addition, the results of immunogold electron microscopy demonstrated that the 3 recombinant baculoviruses displayed the C, E and CE proteins of HCV on the viral surface, respectively. To further define the role of these recombinant baculoviruses in detecting HCV at early stages, we applied time-resolved fluoroimmunoassay (TRFIA) to detect HCV samples after purification and concentration of the recombinant baculoviruses. The results showed that the positive rate of vAc-Flag-CE-GP64 was up to 80.2%. CONCLUSION: vAc-Flag-CE-GP64 is useful for detecting the hepatitis C virus at early stages.     

爬地柏扦插试验初探

文章利用地窖温床扦插爬地柏试验,探索生根粉的外部因素和内在因子,对促进生根、激素种类、溶液深度、浸泡时间、基质种类、取枝部位、插穗年龄及母树年龄等因子进行筛选,提出最佳处理组合,生根率可达90%以上。     

樟子松温室容器育苗技术

樟子松温室容器育苗出苗率达95%以上,表现为生长快,造林成活率高,通过温室容器育苗缩短育苗周期,经济效益显著。     

Genetic Diversity and Global Distribution of Citrus tristeza virus （CTV） Strains

Citrus tristeza virus （CTV）, the most devastating viral pathogen in citrus, causes tremendous economic losses to citrus industry worldwide. The CTV isolates exhibit variable pathogenicities on their ho...     

著作权侵权行为归责原则体系之研究

学术界对于著作权侵权行为归责原则体系存在争议,研究著作权侵权责任归责原则,直接关系到著作权法的适用,以及著作权侵权案件的正确处理。确立我国著作权侵权的归责原则,既要考虑与国际接轨的因素,又要考虑本国的实际情况,构建以过错责任原则为基础,以过错推定原则为补充,在法律特别规定的情况下,可以适用无过错责任归责原则的体系应当是现阶段我国较为合适的选择。     

河西走廊农地适度经营规模计量研究——基于578农户调查的研究

研究适度农地经营规模确定方法并合理确定河西走廊农地适度经营规模。运用生产函数模型对农户劳均经营规模和户均经营规模进行计量分析。研究结果:1)河西地区农业生产处于规模报酬递减阶段。2)在劳动力、资本和土地三大投入要素中,土地对产量的贡献最大。3)现有技术条件下,河西地区劳均最优规模和户均最优规模均远远大于现有规模。研究结论:加快土地流转和剩余劳动力转移促进农地规模经营。