Detection of HCV core protein,envelope glycoprotein and core-envelope fusion protein by baculovirus surface display

AIM: To detect the hepatitis C virus (HCV) at early stages by the technique of baculovirus surface display. METHODS: We constructed the recombinant baculoviruses vAc-Flag-HCV C-GP64, vAc-Flag-HCV E-GP64 and vAc-Flag-CE-GP64, which displayed HCV-related structural proteins by the technique of baculovirus surface display. RESULTS: Western blotting results showed that the core (C), envelope (E) and core-envelope (CE) proteins of HCV were expressed in Sf9 cells after the infection of recombinant baculoviruses. We also observed by confocal microscopy that the C, E and CE proteins of HCV presented and were anchored on the plasma membrane of Sf9 cells after infection. In addition, the results of immunogold electron microscopy demonstrated that the 3 recombinant baculoviruses displayed the C, E and CE proteins of HCV on the viral surface, respectively. To further define the role of these recombinant baculoviruses in detecting HCV at early stages, we applied time-resolved fluoroimmunoassay (TRFIA) to detect HCV samples after purification and concentration of the recombinant baculoviruses. The results showed that the positive rate of vAc-Flag-CE-GP64 was up to 80.2%. CONCLUSION: vAc-Flag-CE-GP64 is useful for detecting the hepatitis C virus at early stages.     

著作权侵权行为归责原则体系之研究

学术界对于著作权侵权行为归责原则体系存在争议,研究著作权侵权责任归责原则,直接关系到著作权法的适用,以及著作权侵权案件的正确处理。确立我国著作权侵权的归责原则,既要考虑与国际接轨的因素,又要考虑本国的实际情况,构建以过错责任原则为基础,以过错推定原则为补充,在法律特别规定的情况下,可以适用无过错责任归责原则的体系应当是现阶段我国较为合适的选择。     

河西走廊农地适度经营规模计量研究——基于578农户调查的研究

研究适度农地经营规模确定方法并合理确定河西走廊农地适度经营规模。运用生产函数模型对农户劳均经营规模和户均经营规模进行计量分析。研究结果:1)河西地区农业生产处于规模报酬递减阶段。2)在劳动力、资本和土地三大投入要素中,土地对产量的贡献最大。3)现有技术条件下,河西地区劳均最优规模和户均最优规模均远远大于现有规模。研究结论:加快土地流转和剩余劳动力转移促进农地规模经营。     

优质高产三系杂交早籼稻新组合中9优547的选育与思考

中9优547是湖北省黄冈市农科院用不育系中9A与恢复系R547配组育成的三系杂交早籼稻新组合,2010年通过湖北省农作物品种审定委员会审定。具有米质优、高产稳产、生育期适宜、制种产量高等优点,适宜于在稻瘟病、白叶枯病和纹枯病无病区或轻发区种植。     

青海半地下式日光节能温室结构及建造

介绍了半地下式日光节能温室的结构,根据青海省气候特点,进行优化设计,提出了半地下日光节能温室墙体厚度、后屋面、畦面下切1.4-1.5m等结构设计参数,以及温室建设的技术及流程。     

长春市花卉业发展的展望

分析了长春市花卉业的现状、水平及发展化卉业的条件及优势。讨论了长春市发展花卉产业的基本设想和对策。     

保护利用瓢虫防治甘蔗绵蚜

1982～1985年,在广西武鸣华侨农场进行保护利用瓢虫控制蔗田绵蚜为害试验。主要措施是保护安全越冬、越夏。冬季室内饲养,室温18～20℃,相对湿度75～85％;翌年4月,大突肩瓢虫越冬存活率为62,96～82.92％;双带盘瓢虫存活率45.71～100％。保护安全越夏的最适室温为23～26℃,相对湿度75～85％,大突肩瓢虫越夏存活率为69.13％(当年第一代成虫)和53.8％(越冬代成虫)。双带盘瓢虫抗逆性较强,在一般室内即能越夏。4年来累计释放两种瓢虫的成、幼虫共44661头,防治蔗田绵蚜效果明显,减少蚜害株率96.6％、蚜害叶片率90％。     

祁连山林区土壤水分条件的分析与评价

通过对祁连山林区云杉林、圆柏林、灌丛林和牧草地等4种主要植被类型土壤水分动态进行长期定位研究,揭示出生长季节内各植被类型的土壤水分动态变化规律.从土壤水分的变化特征,土壤水分有效性等几个方面对祁连山林区主要植被类型的土壤水分条件进行了分析与评价,表明祁连山林区主要植被类型的土壤水分垂直变化可分为土壤水分弱利用层、土壤水分利用层、土壤水分调节层;林地土壤水分的季节变化可分为土壤水分消耗期、土壤水分补偿期、土壤水分消退期、土壤水分稳定期4个时期.根据土壤水分对植物的有效性,将土壤水分划分为易效水、中效水和难效水三种状态,评价土壤水分有效性认为云杉林水分供应状况最好.     

温度对玉米纹枯病菌生长与发病的影响

室内控制性测定结果表明,玉米纹枯病菌(RhizoctoniasolaniAG1鄄IA)菌丝生长的温度10～35℃,最适温度25～30℃;菌核萌发温度18～35℃,最适温度25～30℃。在幼苗和离体叶鞘上由菌丝引起侵染和发病的温度15～35℃,以25～30℃最适宜,潜育期最短,病斑较大。田间分期播种观察分析,气温和地面温度与病害始见的历期(播种期至病害初见期)分别呈显著负相关和极显著负相关。在田间自然条件下,始病的温度为20℃左右。这些结果为了解玉米纹枯病的发生规律,开展预测预报提供了科学依据。     

Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments

Broomrapes (Orobanche spp.) are parasitic weeds that cause significant losses of crop yield. Experiments were conducted to investigate the seed response to the artificial germination stimulant GR₂₄ in three species of Orobanche subjected to preconditioning under various temperatures, water potentials and with plant growth regulators. The highest germination percentages were observed in Orobanche ramosa, Orobanche aegyptiaca and Orobanche minor seeds conditioned at 18°C for 7 days followed by germination stimulation at 18°C. With the increase of the conditioning period (7, 14, 21 and 28 days), the germination percentage of O. ramosa and O. aegyptiaca progressively decreased. When conditioned at −2 MPa, the germination percentage was lower than at 0 and −1 MPa, especially at 13 and 28°C. Orobanche minor seeds could retain relatively high germination if conditioned at 18, 23 or 28°C, even after significantly extended conditioning periods (up to 84 days). GA₃ (30–100 mg L⁻¹), norflurazon and fluridone (10–100 mg L⁻¹), and brassinolide (0.5–1.0 mg L⁻¹) increased seed germination, while 0.01 mg L⁻¹ uniconazole significantly reduced germination rates of all three Orobanche spp. The promotional effects of GA₃ and norflurazon and the inhibitory effect of uniconazole were evident, even when they were treated for 3 days. Germination of Orobanche seeds was much lower when the unconditioned seeds were directly exposed to GR₂₄ at 10⁻⁶ m . This early GR₂₄-induced inhibition was however alleviated or even eliminated by the inclusion of GA₃ or norflurazon (10–50 mg L⁻¹) in the conditioning medium. On the contrary, the inclusion of uniconazole increased the inhibitory effect of GR₂₄, particularly in the case of O. ramosa.