高效液相色谱与电喷雾电离质谱联用测定婴儿配方奶粉中的牛磺酸和维生素

目的利用高效液相色谱与电喷雾电离质谱联用技术,建立一种同时测定婴儿配方奶粉中的牛磺酸和4种维生素(维生素B1、维生素B5、维生素B6、维生素PP)含量的分析方法。方法色谱条件采用RP-C18色谱柱,流动相为5mmol/L七氟丁酸和甲醇,梯度洗脱。质谱采用电喷雾正离子模式(ESI ),以马尿酸为内标定量。结果4种维生素和牛磺酸含量测定方法的线性关系良好(r=0.9996～0.9999,最低定量限10μg/L),前处理技术简单,用该法测定了婴儿配方奶粉中的牛磺酸和4种维生素的含量,回收率为99.1%～101.7%。结论本方法操作简便,精密度好,结果准确可靠。     

Construction of a lentiviral RNA interference vector targeting rat myocardin and its effect on vascular smooth muscle cell differentiation

AIM: To construct a lentiviral RNA interference(RNAi)vector targeting rat myocardin mRNA and to investigate its effect on the differentiation of vascular smooth muscle cells(VSMCs).METHODS: Three pairs of dsDNA targeting rat myocardin mRNA were designed, synthesized and cloned into lentiviral vector pGCSIL-GFP to generate pGCSIL-GFP-shMyocd lentvirus. A Flag-tagged myocardin-overexpression vector pEGFP-N1-Myocd was constructed with pEGFP-N1/X124G. After these two vectors were cotransfected into 293T cells, the flag protein was assessed by Western blotting to analyze the knockdown effect of pGCSIL-GFP-shMyocd. The expression of myocardin and SM22α was also detected by RT-PCR and Western blotting after the pGCSIL-GFP-shMyocd viruses were transfected into primary cultured rat aortal VSMCs.RESULTS: The rat myocardin lentviral RNAi vector pGCSIL-GFP-shMyocd and myocardin-overexpression vector pEGFP-N1-Myocd were successfully constructed. After these two kinds of vectors were cotransfected into 293T cells,the No.1 interfering vector displayed the highest inhibitory effect on flag expression.After the No.1 lentvirus at the titer of 1×10¹² TU/L was transfected into VSMCs, the myocardin and SM22α expression was significantly attenuated. CONCLUSION: The lentiviral pGCSIL-GFP-shMyocd RNAi vector is successfully constructed, which is useful for further study regarding the molecular mechanism of the phenotypic switching in VSMCs under special pathological conditions such as atherosclerosis. Inhibition of myocardin expression in VSMCs leads to the decrease in the expression of differentiation marker, and implies a crucial role of myocardin in VSMCs differentiation.     

pH对针铁矿表面磷酸盐吸附的化学形态的影响

Chemical forms of the phosphate adsorbed on goethite surfaces and characteristics of the coordinate groups which exchange with P on goethite surfaces in solutions with different pll values were investigated.Results showed that the chemical forms of P on goethite surfaces changed from the dominance of monodentate corrdination to that of bidentate one with increasing pH of the solution.By influencing types of phosphate ions in solutions,pH affected the chemical forms of P on goethite surfaces,The amount of OH^- displaced by phosphae on goethite surfaces was the most at pH 7.0,the second at pH 9.0,and the least at pH 4.5.     

岩滩水库浮游植物多样性与环境因子的灰色关联性分析

对4种不同类型水域(江栋、巴龙岛、上音屯、汉达)进行周年(2012年2月至2013年3月)采样监测,并对浮游植物多样性(H)与环境因子进行序列间的灰色关联分析,探究影响岩滩水库浮游植物多样性的主要环境因素及其影响的强弱。结果表明,岩滩水库(各监测水域)浮游植物Shannon-Wiener多样性指数(H)介于1.39~3.17之间,其水平变化表现为上音屯(围栏养殖区)>汉达(主河道非养殖区)>巴龙岛(网箱养鱼次密集区)>江栋(网箱养鱼密集区)。监测因子中,温度与水体浮游植物多样性的关联度最高、为0.774;其次为pH、CODMn和NO2-N,关联度分别为0.685、0.628和0.578;TP和BOD5对浮游植物群落结构的影响相对较小,关联度分别为0.498和0.354。相对密集的网箱养鱼使养殖区浮游植物特定种(属)类种群密度及生物量增大,而生物多样性水平(H)下降。     

玉米秸秆腐解对石灰性褐土酶活性和有机质质量分数动态变化的影响

通过室内培养,研究玉米秸秆腐解量与腐解时间的关系,以及不同秸秆添加量对石灰性褐土酶活性和有机质质量分数动态变化的影响。结果表明,玉米秸秆的腐解量随培养时间延长而增加,在培养的第45天,腐解量可达45%左右,而45天后腐解速度明显下降。在腐解的前60d,常规秸秆添加处理的腐解速率小于加倍量秸秆添加量处理,而在培养后期处理间腐解速率无差异,且腐解速率均随腐解时间延长而减小。土壤过氧化氢酶活性随培养时间的延长呈先增加后降低再增加的趋势,但不同秸秆量处理土壤过氧化氢酶活性差异不显著;碱性磷酸酶活性随秸秆添加量增加而增大,且均随培养时间延长而提高。土壤有机质和活性有机质质量分数均随培养时间延长而增加,且随秸秆添加量的增加而显著增加。     

三叉苦叶提取物的抑菌活性研究

以金黄色葡萄球菌、枯草芽孢杆菌、绿脓杆菌、青霉、黑曲霉为供试菌种,采用滤纸扩散法、平板稀释法,对三叉苦叶提取液的抑菌活性进行研究.结果表明,三叉苦叶提取液对细菌具有较强抑制作用,对两种真菌无抑菌作用;对细菌的最低抑制浓度(MIC)范围在0.125～0.5 g/mL之间;三叉苦叶提取液的抗菌活性具有热稳定性,并且在pH值3.5～9范围内均有抗菌活性,碱性条件下抑菌作用相对较强.     

木薯高产栽培技术

福建省明溪县适宜木薯生长,是木薯的主产区.该文总结了木薯的生物学特性以及高产栽培技术.     

一种新型设施草莓立体栽培模式的探讨

为提高设施草莓土地利用率,设计一种新型设施草莓立体栽培模式("新H型":即"H型"架下土壤栽培一层草莓,形成双层立体栽培模式),以‘红颜’草莓为试验材料,分析对比在"新H型"和传统栽培2种不同栽培模式下的光照条件、植株营养生长、果实产量与品质、经济效益等指标,并综合评价了"新H型"立体栽培模式实际生产性能。结果表明:单位面积土地上,"新H型"立体栽培模式比传统栽培模式种植株数多20%,投入增加44.455%,产量增加20.240%,总收入增加35.269%,纯利润增加25.876%。初步证实,"新H型"立体栽培模式整体表现优于传统栽培,提高了土地利用效率。     

深圳坝光银叶树湿地园林木有害生物调查及防控

对坝光银叶树Heritiera littoralis 湿地园开展有害生物调查,收集并鉴定有害生物72 种,其中有害杂草5 种、寄生植物2 种、病害20 种、虫害45 种。红火蚁Solenopsis invicta 和薇甘菊Mikania micrantha 属于外来入侵检疫性有害生物,八点广翅蜡蝉Ricania speculum 在公园内危害严重,对主要有害生物应加强预测预报、及时防治。     

喷施亚硒酸钠对紫花苜蓿干草产量和品质的影响

采用叶面喷施的方法研究了3个不同施硒水平(50、100、200 mg/kg)对紫花苜蓿Medicaco sativa干草产量及品质的影响.结果表明:一年4茬每茬次干草产量都随着施硒量的增加有降低的趋势,但对不同茬次的干草产量影响不同,适量喷施硒肥可以显著提高紫花苜蓿产量(P＜0.05),年产量以施肥量50 mg/kg的亚硒酸钠最高.喷施硒肥对每茬次营养物质含量的影响不同,4个处理紫花苜蓿粗蛋白、粗灰分及植株磷含量之间差异不显著,粗纤维及粗脂肪含量则随着喷施硒量的增加而增加,粗纤维以100 mg/kg处理最高,粗脂肪以200 mg/kg处理最高,但紫花苜蓿营养物质年积累量都随着施硒量的增加而降低,其中以50 mg/kg处理最高,要显著高于对照.