高寒牧区牧鸡鸭治蝗效果试验

在青海湖北岸高寒牧区利用人工饲养的鸡鸭进行放牧防治草原蝗虫。结果表明,在60d的防治期内,牧鸡鸭平均灭治效果达91.4%,为人工利用天敌治虫技术在高寒牧区的应用提供了依据。     

浅谈HACCP在机制针形名茶中的应用

对HACCP作简要介绍，根据HACCP体系的要求，分析了机制针形名茶过程中各个环节可能出现的危害因素，用HACCP的原理确立了关键控制点，并且建立了相应的控制措施。     

黄瓜枯萎病新型抑制蛋白L37的研究

 从土壤中分离到对黄瓜枯萎病病原菌有抑制作用的枯草芽孢杆菌(Bacillus subtilis) , 发酵产物经DEAE - 纤维素、Sephadex G-100柱层析, 分离到15 kD抑菌蛋白L37, SDS-PAGE显示纯度达到电泳纯, N - 末端氨基酸序列同源性检测结果提示可能是一种新型的抑菌蛋白。蛋白质抑菌结果表明, 对多种病原真菌具有抑制效果, 且对蛋白酶、温度和大部分有机物不敏感, 是稳定的抑菌蛋白。对病原菌菌丝抑制机理研究表明, 抑菌蛋白具有严重扭曲、断裂、阻止生长等抗生效果。应用发酵蛋白产物进行两年大田防效试验表明, 对黄瓜枯萎病防效显著, 最高达到90% , 具有显著提高产量的作用。     

压力蒸汽灭菌器对液体灭菌时常见故障浅析

针对压力蒸汽灭菌器对液体进行灭菌时造成事故的现象，分析了事故发生的主要原因是由于限压阀瞬间降压造成的。指出应慎用于液体的灭菌处理。     

荔枝生产相关的水分生理指标远程监测研究

利用植物远程监测系统对荔枝园中的大气温湿度、土壤湿度、光照强度、大气蒸汽压差(VPD)等环境因子和茎干直径微变化、果实生长、叶片温度等树体生理指标进行了监测,发现该系统能准确及时并无伤害地记录他们的实时和周期性变化。据获得的数据初步表明,当果实处于快速膨大期,主茎的加粗生长明显缓慢,一旦果实采收后,茎干加粗生长则有一个迅速上升的变化;当土壤湿度高于33%(v/v)时,主茎的生长受到明显的抑制;VPD白天上升,夜间下降,当夜间VPD始终高于0,就形成所谓的“夜间干燥”,VPD的这种变化对荔枝主茎和果实的生长以及叶气温差(LATD)都有较大的影响,如主茎和果实的日间收缩量随VPD的增大而增大,夜间空气干燥不但抑制了主茎生长,也使日最大LATD下降。据此认为,果实和茎干的生长之间存在竞争关系,土壤湿度过高或者夜间空气干燥对荔枝的生长不利。     

在不同提取方法下蜜蜂基因组DNA浓度的比较

本研究采用了国内外常见的5种蜜蜂基因组DNA抽提的方法,对意大利蜂工蜂基因组DNA进行提取,并进行了浓度检测.其中方法1,方法2采用的是5日龄的工蜂蛹体,而其他3种方法采用的是成年工蜂的头胸部进行提取.试验结果表明这5种方法之间差异极显著,其中方法2的浓度最高,为84.7850±24.5974,说明在相同实验条件下,方法2(蜜蜂蛹体一次抽提法)在浓度层次上效果最好.     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

渭河阶地全新世土壤粒度成分高分辨率研究

通过对渭河阶地全新世土壤的野外考察和粒度成分的高分辨率分析研究，揭示了该区域褐色土的成壤作用过程与季风气候变化的关系。研究表明渭河第二级阶地褐色土形成在全新世气候最适宜期（8500－3100a B.P.），从8500a B.P.开始该土壤开始发育，老官台文化兴起。从3100a B.P.开始的气候干旱化和沙尘暴堆积形成了黄土L。层。西周时期对应于该干旱期的初期。     

丛枝菌根真菌对芋组织培养苗生长的影响

 于温室盆栽条件下研究了丛枝菌根(AM) 真菌Gigaspora rosea、Glomus mosseae 和Glomus versi-forme 对芋(Colocasia esculenta) 组织培养苗移栽成活率、矿质营养、光合速率及生长的影响。结果表明, 接种AM真菌能提高芋组织培养幼苗移栽成活率和叶片光合速率, 降低气孔阻力; 其叶片和根内氮、磷、钾含量和生长量显著高于不接种对照。认为接种有效AM真菌是促进组织培养苗健康生长的重要技术。     

Effects of angiotensin II-induced hypertension chemotherapy on the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas

AIM: To investigate the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas under angiotensin II-induced hypertension chemotherapy. METHODS: The brain tumor model was produced in Wistar rats by stereotaxic inoculation of C6 glioma cells (1×10¹² /L). Tumor-bearing rats were treated with carmustine, teniposide and lisplatin (chemotherapy) during angiotensin II-induced hypertension. Then, the survival time of tumor-bearing rats, tumor blood flow, the concentration of drug, volume of gliomas and the activity of interleukin-1β converting enzyme in glioma were examined.RESULTS: The survival time of tumor-bearing rats was significantly longer in chemotherapy with angiotensin II-induced hypertension group than that of chemotherapy alone. In addition, regional tumor blood flow, the concentration of chemotherapeutic drug and the activity of interleukin -1β converting enzyme in transplanted rat gliomas were increased, while the volume of gliomas was decreased in hypertention chemotherapy group compared with chemotherapy alone. CONCLUSION: Chemotherapy with angiotensin II-induced hypertension has a enhancing effect on chemotherapy for improving the drug delivery to tumor tissue by a increased tumor blood flow and enhancing activity of interleukin -1β converting enzyme.