Beta-adrenergic receptor kinase: primary structure delineates a multigene family

The beta-adrenergic receptor kinase (beta-ARK), which specifically phosphorylates only the agonist-occupied form of the beta-adrenergic and closely related receptors, appears to be important in mediating rapid agonist-specific (homologous) desensitization. The structure of this enzyme was elucidated by isolating clones from a bovine brain complementary DNA library through the use of oligonucleotide probes derived from partial amino acid sequence. The beta-ARK cDNA codes for a protein of 689 amino acids (79.7 kilodaltons) with a protein kinase catalytic domain that bears greatest sequence similarity to protein kinase C and the cyclic adenosine monophosphate (cyclic AMP)--dependent protein kinase. When this clone was inserted into a mammalian expression vector and transfected into COS-7 cells, a protein that specifically phosphorylated the agonist-occupied form of the beta 2-adrenergic receptor and phosphorylated, much more weakly, the light-bleached form of rhodopsin was expressed. RNA blot analysis revealed a messenger RNA of four kilobases with highest amounts in brain and spleen. Genomic DNA blot analysis also suggests that beta-ARK may be the first sequenced member of a multigene family of receptor kinases.     

Skin color and nutrient photolysis: an evolutionary hypothesis

Human populations native to areas of intense sunlight tend to be heavily melanized. Previous explanations for this relationship have invoked only weak selective pressures. To test the hypothesis that dark pigmentation may protect against photolysis of crucial light-sensitive vitamins and metabolites by ultraviolet light, folate was used as a model. It was found that exposure of human plasma in vitro to simulated strong sunlight causes 30 to 50 percent loss of folate within 60 minutes. Furthermore, light-skinned patients exposed to ultraviolet light for dermatologic disorders have abnormally low serum folate concentrations, suggesting that photolysis may also occur in vivo. Deficiency of folate, which occurs in many marginally nourished populations, causes severe anemia, fetal wastage, frank infertility, and maternal mortality. Prevention of ultraviolet photolysis of folate and other light sensitive nutrients by dark skin may be sufficient explanation for the maintenance of this characteristic in human groups indigenous to regions of intense solar radiation.     

Genetic Self-Incompatibility in Oenothera subsect Euoenothera

Although it has been postulated that genetic self-incompatibility was involved in the origin of complex heterozygotes in Oenothera subsect Euoenothera, it has not been detected in any species of this well-studied group. It is now reported for populations of Oenothera grandiflora from west central Alabama, and should be sought in other populations of this species, which has been in cultivation for nearly two centuries.     

Venus: preliminary topographic and surface imaging results from the pioneer orbiter

Three large Venus surface features, identified previously in images obtained from Earth-based radar observations, are shown by the Pioneer Venus radar mapper to be elevated 5 to 10 kilometers above the surrounding terrain. Two of these features, one bright and the other dark, lie adjacent to each other astride the 65 degrees N parallel between longitudes 310 degrees E and 10 degrees E. The combined region forms a huge tectonically uplifted plateau, surmounted by radar-bright ridges that may have either a volcanic or tectonic origin. The third feature, located at 30 degrees N, 283 degrees E, is radar-bright and may consist of volcanic material extruded along a fault zone. A first radar-scattering image, compiled from data obtained by the mapper in its imaging mode, shows a region north of the equator; several circular depressions seen in this area may result from meteoritic impact.     

Strain-softening instability model for the san fernando earthquake

Changes in the ground elevation observed before and immediately after the 1971 San Fernando, California, earthquake are consistent with a theoretical model in which fault zone rocks are strain-softening after peak stress. The model implies that the slip rate of the fault increased to aboul 0.1 meter per year near the focus before the earthquake.     

Crystallographic structure of the octameric histone core of the nucleosome at a resolution of 3.3 A

The structure of the (H2A-H2B-H3-H4)2 histone octamer has been determined by means of x-ray crystallographic techniques at a resolution of 3.3 angstroms. The octamer is a prolate ellipsoid 110 angstroms long and 65 to 70 angstroms in diameter, and its general shape is that of a rugby ball. The size and shape are radically different from those determined in earlier studies. The most striking feature of the histone octamer is its tripartite organization, that is, a central (H3-H4)2 tetramer flanked by two H2A-H2B dimers. The DNA helix, placed around the octamer in a path suggested by the features on the surface of the protein, appears like a spring holding the H2A-H2B dimers at either end of the (H3-H4)2 tetramer.     

Seal lungs collapse during free diving: evidence from arterial nitrogen tensions

Arterial blood nitrogen tensions of free-diving Weddell seals (Leptonychotes weddelli) were measured by attaching a microprocessor-controlled blood pump and drawing samples at depth to determine how these marine mammals dive to great depths and ascend rapidly without developing decompression sickness. Forty-seven samples of arterial blood were obtained from four Weddell seals during free dives lasting up to 23 minutes to depths of 230 meters beneath the sea ice of McMurdo Sound, Antarctica. Peak arterial blood nitrogen tensions of between 2000 and 2500 millimeters of mercury were recorded at depths of 40 to 80 meters during descent, indicating that the seal's lung collapses by 25 to 50 meters. Then arterial blood nitrogen tensions slowly decreased to about 1500 millimeters of mercury at the surface. In a single dive, alveolar collapse and redistribution of blood nitrogen allow the seal to avoid nitrogen narcosis and decompression sickness.     

Atom Pair Distribution Functions of Liquid Water at 25{degrees}C from Neutron Diffraction

The structure of liquid water is described by three atom pair distribution functions gOO(r), gOH(r), and gHH(r). These functions have now been derived from neutron diffraction data on four mixtures of light and heavy water. They will provide a crucial and sensitive test for proposed models of liquid water.     

Control of structure and growth of polymorphic crystalline thin films of amphiphilic molecules on liquid surfaces

The spontaneous formation and coexistence of crystalline polymorphic trilayer domains in amphiphilic films at air-liquid interfaces is demonstrated by grazing incidence synchrotron x-ray diffraction. These polymorphic crystallites may serve as models for the early stages of crystal nucleation and growth, helping to elucidate the manner in which additives influence the progress of crystal nucleation, growth, and polymorphism and suggesting ways of selectively generating and controlling multilayers on liquid surfaces. Auxiliary molecules have been designed to selectively inhibit development of the polymorphs, leading primarily to a single phase monolayer.