全文获取类型
收费全文 | 103257篇 |
免费 | 5641篇 |
国内免费 | 66篇 |
专业分类
林业 | 3603篇 |
农学 | 2891篇 |
基础科学 | 680篇 |
12104篇 | |
综合类 | 17664篇 |
农作物 | 3830篇 |
水产渔业 | 4957篇 |
畜牧兽医 | 55866篇 |
园艺 | 1104篇 |
植物保护 | 6265篇 |
出版年
2018年 | 1344篇 |
2017年 | 1563篇 |
2016年 | 1441篇 |
2015年 | 1199篇 |
2014年 | 1526篇 |
2013年 | 3797篇 |
2012年 | 2720篇 |
2011年 | 3423篇 |
2010年 | 2204篇 |
2009年 | 2272篇 |
2008年 | 3329篇 |
2007年 | 3114篇 |
2006年 | 3045篇 |
2005年 | 2694篇 |
2004年 | 2608篇 |
2003年 | 2649篇 |
2002年 | 2435篇 |
2001年 | 3326篇 |
2000年 | 3399篇 |
1999年 | 2593篇 |
1998年 | 1028篇 |
1997年 | 1050篇 |
1995年 | 1219篇 |
1994年 | 1067篇 |
1993年 | 1018篇 |
1992年 | 2275篇 |
1991年 | 2504篇 |
1990年 | 2262篇 |
1989年 | 2257篇 |
1988年 | 2132篇 |
1987年 | 2235篇 |
1986年 | 2253篇 |
1985年 | 2157篇 |
1984年 | 1742篇 |
1983年 | 1518篇 |
1982年 | 1064篇 |
1979年 | 1680篇 |
1978年 | 1358篇 |
1977年 | 1199篇 |
1976年 | 1177篇 |
1975年 | 1243篇 |
1974年 | 1527篇 |
1973年 | 1559篇 |
1972年 | 1455篇 |
1971年 | 1340篇 |
1970年 | 1261篇 |
1969年 | 1206篇 |
1968年 | 1066篇 |
1967年 | 1147篇 |
1966年 | 1004篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Paraud C Gaudin C Pors I Chartier C 《Veterinary journal (London, England : 1997)》2009,180(2):265-267
Paramphistome infections are very common in ruminants and may induce clinical signs, but little is known about effective treatments. In this study, the efficacy of oxyclozanide against Calicophoron daubneyi was studied in goats and its activity tested against immature stages (10 days post-infection) at a dose of 22.5mg/kg bodyweight (BW) and against adult stages using two doses (15 and 22.5mg/kg BW). There was a reduction (82%) in the number of immature worms (compared to controls) but the result was not statistically significant. When tested against adult stages, however, oxyclozanide reduced the worm burdens by 95.6% and 95.9% at doses of 15 and 22.5mg/kg BW, respectively, with no significant difference between the two doses. The experiment demonstrated that oxyclozanide is highly effective in reducing the number of adult paramphistomes in goats. 相似文献
992.
The sialyl transferase of disialoganglioside formation is depressed in mammary tumors induced in the rat by 7,12-dimethylbenz[a]anthracene. Specific activities of other glycosyltransferases of the pathway ceramide to monosialo-ganglioside are unchanged or elevated so that the ganglioside GM(1) accumulates and higher gangliosides are depressed. These findings with a solid tumor are critical to an involvement of gangliosides in the cell-surface changes of tumorigenesis. 相似文献
993.
Fossler CP Wells SJ Kaneene JB Ruegg PL Warnick LD Bender JB Eberly LE Godden SM Halbert LW 《Preventive veterinary medicine》2005,70(3-4):279-291
The objective of this study was to evaluate associations between herd-level factors and the isolation of Salmonella in calves from dairy farms in Minnesota, Wisconsin, Michigan and New York. Study farms were 129 conventional and organic farms enrolled without regard to previous history of Salmonella infection. Herds were sampled at 2-month intervals over a 1-year period. Salmonella was isolated in fecal samples from 176 (3.8%) of 4673 preweaned calves with 40 (31.0%) of 129 farms having at least one positive calf sample over the course of the study. Multivariable logistic regression using the generalized estimating equations approach was used to evaluate risk factors for Salmonella shedding after adjustment for effects of herd size, season, state of origin and the multiple sampling occasions per herd. Factors retained in the final model that were associated with an increased odds for Salmonella shedding were lack of routine feeding of milk replacer containing antimicrobials to preweaned calves (OR = 2.8, 95% CI: 1.4, 5.8), use of maternity housing as a hospital area for sick cows more than once a month (OR = 2.1, 95% CI: 1.1, 4.0), and cow prevalence level by visit, categorized into the following four-levels: ≥20% (OR = 11.6, 95% CI: 5.7, 23.7), 10–19.9% (OR = 4.7, 95% CI: 2.0, 11.5), 0.1–9.9% (OR = 3.6, 95% CI: 1.5, 8.7) and 0% (reference level). Herd size was not associated with Salmonella shedding in the final multivariable model. 相似文献
994.
The occurrence of genetic male sterility and development of highly sterile lines have been reported. The male sterility was not accompanied by any visible chromosomal aberration. It behaved as recessive and was governed by multiple factors. It was hypothesised that three major genes with additive effect were operating to produce highly sterile forms while less sterile forms would be dependent on one or two genes. The expression of male sterility was also influenced by modifying factors and environments. 相似文献
995.
The objective of this study was to determine the effect of feeding Ascophyllum nodosum (ANOD) to lambs at 0 (control), 0.5, 1, or 2% of DMI/d for 2 wk on lamb physiology in response to forced walking and transport during hot weather. Forty-four lambs (26 kg +/- 4.3) were used, and each lamb swallowed 3 gelatin capsules filled with ANOD or their normal 16% CP, pelleted grain ration twice daily, with the amount of ANOD dependent on the treatment. The amount of ANOD did not affect ear canal temperature or cortisol concentrations during 60 min of forced walking. The range between the minimum and maximum ear canal temperature for each lamb during 12 h of transport was narrower in lambs receiving the 2% ANOD than the control group (P = 0.05), and the 2% ANOD group also had lower (P = 0.05) ear canal temperatures than the control group during hot periods of transport. After 4 (P = 0.09) and 8 h (P = 0.05) of transport, the control group tended to have greater cortisol concentrations than the 2% ANOD group. Many differences among treatments were found in plasma protein, albumin, calcium, phosphorus, glucose, blood urea nitrogen, aspartate aminotransferase, magnesium, sodium, potassium, and chloride concentrations posttransport; mainly, the control and 0.5% ANOD groups had greater (P < 0.05) concentrations than the other 2 treatments. Aldosterone concentrations were greater in the control and 0.5% ANOD group than in the 1 and 2% ANOD groups before transport, whereas the concentrations were not different after transport, suggesting pretransport concentrations were lowered by supplementation. The 1 and 2% ANOD groups lost more BW than the control group as a result of transport (P = 0.04). After transport, no differences were observed in the latency for lambs to drink, eat, or lay. There was a suppression of the IgG and IgM antibody responses at 4 and 7 d after administration of ovalbumin, with greater ANOD supplementation rates suppressing antibody response the greatest. Although ANOD decreased ear canal temperature in hot periods of transport, stabilized electrolyte concentrations, and decreased cortisol throughout transport, it also suppressed the antibody response indicating that the effect of ANOD on immune function merits further investigation. 相似文献
996.
Comparative evaluation of MCP gene in worldwide strains of Megalocytivirus (Iridoviridae family) for early diagnostic marker 下载免费PDF全文
A Mishra G‐H Nam J‐A Gim H‐E Lee A Jo D Yoon S Oh S Kim A Kim D‐H Kim Y C Kim H D Jeong H‐J Cha Y H Choi H‐S Kim 《Journal of fish diseases》2018,41(1):105-116
Members of the Iridoviridae family have been considered as aetiological agents of iridovirus diseases, causing fish mortalities and economic losses all over the world. Virus identification based on candidate gene sequencing is faster, more accurate and more reliable than other traditional phenotype methodologies. Iridoviridae viruses are covered by a protein shell (capsid) encoded by the important candidate gene, major capsid protein (MCP). In this study, we investigated the potential of the MCP gene for use in the diagnosis and identification of infections caused Megalocytivirus of the Iridoviridae family. We selected data of 66 Iridoviridae family isolates (53 strains of Megalocytivirus, eight strains of iridoviruses and five strains of Ranavirus) infecting various species of fish distributed all over the world. A total of 53 strains of Megalocytivirus were used for designing the complete primer sets for identifying the most hypervariable region of the MCP gene. Further, our in silico analysis of 102 sequences of related and unrelated viruses reconfirms that primer sets could identify strains more specifically and offers a useful and fast alternative for routine clinical laboratory testing. Our findings suggest that phenotype observation along with diagnosis using universal primer sets can help detect infection or carriers at an early stage. 相似文献
997.
Claire N. Lieske James H. Clark Howard G. Meyer Leigh Boldt Martin D. Green John R. Lowe Walter E. Sultan Peter Blumbergs Matthew A. Priest 《Pesticide biochemistry and physiology》1984,22(3):285-294
The inhibition of eel acetylcholinesterase by the 4-nitrophenyl esters of 2-furyl(methyl)-, methyl(2-thienyl)-, di-2-furyl-, and di-2-thienylphosphinic acid (I, II, III, and IV, respectively) was investigated at pH 6.90 in 0.067 M phosphate buffer (25.0°C) using stopped-flow instrumentation and automated data processing. Our evaluation of the dissociation constant, Kd, the unimolecular bonding rate constant, k2, and the bimolecular reaction constant, ki, are the first reported values for these constants for alkyl/heteroaryl and diheteroaryl esters of phosphinic acids. The largest ki value (19,330 M?1 sec?1) was observed for the reaction of I with the enzyme. The order for the remaining three is II > IV > III. There is no direct relationship between the hydrolysis rates of the esters and their anticholinesterase activities on eel acetylcholinesterase. Likewise, there is no direct relationship between their anticholinesterase activities and the LD50 values in rats. 相似文献
998.
Katherine I O'Rourke Dongyue Zhuang Amy Lyda Gabriel Gomez Elizabeth S Williams Wenbin Tuo Michael W Miller 《Journal of veterinary diagnostic investigation》2003,15(4):320-323
A monoclonal antibody dot-blot assay was used to evaluate detergent lysates of tonsil tissue from mule deer to detect PrP(CWD), the marker for the cervid transmissible spongiform encephalopathy chronic wasting disease (CWD). Samples of formalin-fixed brain and tonsil tissues from mule deer were examined for PrP(CWD) using immunohistochemistry (IHC) with Mab F99/97.6.1, the gold standard for diagnosis of preclinical CWD. The contralateral tonsil from each of the 143 deer was prepared for confirmatory IHC and as a 10% (wt/vol) detergent lysate without purification or enrichment steps for monoclonal antibody dot-blot assay. PrP(CWD) was detected by dot-blot assay in 49 of 50 samples considered positive by IHC. Forty-eight of the positive samples were evaluated with a quantitative dot-blot assay calibrated with recombinant PrP. Tonsillar PrP(CWD) concentrations ranged from 34 to 1,188 ng per 0.5 mg starting wet weight of tissue. The abundant PrP(CWD) in mule deer tonsil will facilitate development and validation of high-throughput screening tests for CWD in large populations of free-ranging deer. 相似文献
999.
Hamir AN Richt JA Miller JM Kunkle RA Hall SM Nicholson EM O'Rourke KI Greenlee JJ Williams ES 《Veterinary pathology》2008,45(3):297-306
To compare clinical and pathologic findings of chronic wasting disease (CWD) in a natural host, 3 groups (n = 5) of white-tailed deer (WTD) fawns were intracerebrally inoculated with a CWD prion of WTD, mule deer, or elk origin. Three other uninoculated fawns served as controls. Approximately 10 months postinoculation (MPI), 1 deer from each of the 3 inoculated groups was necropsied and their tissues were examined for lesions of spongiform encephalopathy (SE) and for the presence of abnormal prion protein (PrP(d)) by immunohistochemistry (IHC) and Western blot (WB). The remaining deer were allowed to live until they developed clinical signs of the disease which began approximately 18 MPI. By 26 MPI, all deer were euthanatized on humane grounds. Obvious differences in clinical signs or the incubation periods were not observed between the 3 groups of deer given CWD. In 1 of 3 nonclinical deer euthanatized at 10 MPI, minimal microscopic lesions of SE were seen in the central nervous system (CNS) tissues, and PrP(d) was observed by IHC in tissues of all 3 deer. In the clinical deer, CNS lesions of SE and PrP(d) accumulations were more severe and extensive. It is concluded that the 3 sources of CWD prion did not induce significant differences in time to clinical disease or qualitative differences in signs or lesions in WTD. However, this observation does not imply that these CWD agents would necessarily behave similarly in other recipient species. 相似文献
1000.