The relation between polyphenoloxidase activity and ability to produce indoleacetic acid in Fusarium-infected tomato plants

Different protein fractions precipitated with ammonium sulphate from extracts of healthy andFusarium-infected tomato plants showed a linear relationship between polyphenoloxidase activity and ability to form indoleacetic acid (IAA) from tryptophan in the presence of chlorogenic acid. Dialysis slightly reduced polyphenoloxidase activity of as well as IAA formation by crude and purified extracts.Polyphenoloxidase as well as IAA production were partially inhibited by DIECA, EDTA, cystein and ascorbic acid. The inhibition was shown to occur in crude and purified extracts and stem slices of both healthy and diseased plants, but was consistently higher in material from diseased plants.The reported results may be considered to further confirm the strict interdependence between polyphenoloxidase activity and ability to form IAA in tomato plants. The different sensitivity to some inhibitors shown by material from healthy and from diseased plants is emphasized.     

Occurrence of coccidiosis in calves at one to six months of age, which are housed in a large capacity barn]

One- to six-month-old calves, housed in the large-capacity calf house of the Nová Bystrice state farm in the district of Jindrich?v Hradec were studied for the occurrence of coccidia in the period from January 1974 to April 1975. The coccidia found in these animals included nine species of the genus Eimeria: E. zuernii, E. bovis, E. ellipsoidalis, E. subspherica, E. auburnensis. The species E. alabamensis, E. cylindrica, E. wyomingensis and E. bukidnonensis occurred just sporadically. Further, the findings included oocysts of Isopora spp. The oocysts of the coccidia E. wyomingensis and Isopora spp. were found for the first time in Czechoslovakia. The overall extensity of invasion (EI) and intensity of invasion (II) grow with age as follows: (chart: see text) The results suggest that even modern large-capacity calf houses are not free from invasion by coccidia; the calves in the house under study were invaded by ten species. Transition to vegetable feeding is accompanied by a marked rise of the EI of all the species of coccidia found in the animals.