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101.
102.
Bruno Reis Ana Teresa Gonalves Paulo Santos Manuel Sardinha Luís E. C. Conceio Renata Serradeiro Jaume Prez-Snchez Josep Calduch-Giner Ulrike Schmid-Staiger Konstantin Frick Jorge Dias Benjamín Costas 《Marine drugs》2021,19(12)
This work aimed to evaluate the effects of dietary supplementation with β-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different β-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived β-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% β-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 ± 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed β-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-β-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects. 相似文献
103.
104.
Hugo E. Ramírez-Guerra Enrique Márquez-Ríos Guadalupe M. Suárez-Jiménez Ofelia Rouzaud-Sández María E. Lugo-Sánchez Juan C. Ramírez-Suárez 《Journal Of Aquatic Food Product Technology》2019,28(3):275-286
Physicochemical and structural properties of soluble jumbo squid (Dosidicus gigas) elastin recovered from skin by-products were evaluated. The molecular weight of isolated elastin was ~40 kDa with an isoelectric point (pI) between 9 and 10. Aspartic, glutamic, arginine, proline, glycine, and lysine amino acids were the most abundant in squid elastin, whereas the hydroxyproline absence, ~0.7% cysteine content, and the calculated 0.35 isoleucine/leucine ratio were used as purity index. Total and reactive sulfhydryl contents were similar (247.0 ± 5.1 vs 242.0 ± 7.5 μmol mg?1 of protein, P ≥ 0.05) in purified squid elastin but surprisingly higher than previously reported in other elastins. On the other hand, the secondary structures of squid elastin analyzed by Fourier transform infrared spectroscopy (FTIR) were ~45% β-sheets, ~15% α-helices, ~10% β-turns, and ~30% undefined structures. In addition, squid elastin experienced glass transition at 82.01 ± 0.01ºC, denaturation temperature at 110.45 ± 0.64ºC, and aggregation at 197.5 ± 0.23ºC. In conclusion, the prevalence of charged amino acids and pI of squid elastin can facilitate its solubilization in hydrophilic systems, whereas the secondary structure profile and thermostability are desirable features in proteins used for biopolymer designs such as food biofilms or barrier systems. 相似文献
105.
Bruno O. David Matt Jarvis Deniz
zkundakci Kevin J. Collier Andy S. Hicks Malcolm Reid 《水产资源保护:海洋与淡水生态系统》2019,29(9):1409-1423
106.
Kennya Gomes Silva Addam Scheila Anelise Pereira Gabriel Fernandes Alves Jesus Lucas Cardoso Nichola Syracuse Gustavo Ruschel Lopes Nicollas Breda Lehmann Bruno Correa da Silva Lúvia Souza de S Francisco Clio Maia Chaves Maurício Latera Martins Jos Luiz Pedreira Mourio 《Aquaculture Research》2019,50(10):2960-2971
This study evaluated the effects of dietary supplementation of organic acids blend (OAB) alone or in combination with essential oil, Lippia origanoides (OAE) for Nile tilapia fed supplemented diets for 30 days. Fish (1.1 ± 0.04 g) were fed control (Control), or OAB 0.5% or OAB 0.5% + essential oil 0.125% (OAE) respectively. At the end of the experiment, samples were collected for de hemato‐immunological, histological analysis of the intestine and liver, as well as microbiology of the intestine. The pH of the diets supplemented with OAB and OAE reduced 0.92 and 0.19 respectively. The growth and FCR were unaffected by the treatments, but survival was significantly higher in the OAB treatment. Fish fed the OAB diet showed reduced concentration of total heterotrophic bacteria and Pseudomonas sp. in the intestine. Increased glucose in fish fed OAB and high number of circulating monocytes in fish fed OAE diet were observed. The anterior intestine of fish fed OAE diet showed larger number of goblet cells and increased villi height. The diet supplemented with OAB, mainly, improved the intestinal health and survival of tilapia juveniles and can be used in juvenile production. 相似文献
107.
Rmi Cousin Hugo Groult Chanez Manseur Romain Ferru-Clment Mario Gani Rachel Havret Claire Toucheteau Grgoire Prunier Batrice Colin Franck Morel Jean-Marie Piot Isabelle Lanneluc Kvin Baranger Thierry Maugard Ingrid Fruitier-Arnaudin 《Marine drugs》2021,19(10)
Sugar-based molecules such as heparins or natural heparan sulfate polysaccharides have been developed and widely studied for controlling heparanase (HPSE) enzymatic activity, a key player in extracellular matrix remodelling during cancer pathogenesis. However, non-enzymatic functions of HPSE have also been described in tumour mechanisms. Given their versatile properties, we hypothesized that sugar-based inhibitors may interfere with enzymatic but also non-enzymatic HPSE activities. In this work, we assessed the effects of an original marine λ-carrageenan derived oligosaccharide (λ-CO) we previously described, along with those of its native counterpart and heparins, on cell viability, proliferation, migration, and invasion of MDA-MB-231 breast cancer cells but also of sh-MDA-MB-231 cells, in which the expression of HPSE was selectively downregulated. We observed no cytotoxic and no anti-proliferative effects of our compounds but surprisingly λ-CO was the most efficient to reduce cell migration and invasion compared with heparins, and in a HPSE-dependent manner. We provided evidence that λ-CO tightly controlled a HPSE/MMP-14/MMP-2 axis, leading to reduced MMP-2 activity. Altogether, this study highlights λ-CO as a potent HPSE “modulator” capable of reducing not only the enzymatic activity of HPSE but also the functions controlled by the HPSE levels. 相似文献
108.
Schizochytrium as a replacement for fish oil in a fishmeal free diet for jade perch,Scortum barcoo (McCulloch & Waite) 下载免费PDF全文
Stijn Van Hoestenberghe Charles‐Aimé Fransman Tom Luyten Daniel Vermeulen Ivo Roelants Saskia Buysens Bruno M. Goddeeris 《Aquaculture Research》2016,47(6):1747-1760
A 10‐week trial was conducted to determine the response of juvenile jade perch Scortum barcoo on the replacement of dietary fish oil (FO) in a fishmeal free diet. Three iso‐nitrogenous, isocaloric and isolipidic diets were formulated, each containing a different primary fat source: FO, linseed oil (LO), and a mixture of Schizochytrium and LO. The substitution of FO with the mixture of Schizochytrium and LO did not cause a difference in growth. However, there was an 8% reduction in weight gain in fish fed dietary LO, indicating that juvenile jade perch do require a minimal concentration of dietary n‐3 highly unsaturated fatty acids (HUFA). Fish fed the Schizochytrium diet stored more efficient n‐3 HUFA and in particular DHA in their flesh, and retained a higher fillet recovery compared to fish fed FO. In addition, we demonstrated that jade perch are able to produce both n‐3 HUFA and n‐6 HUFA when dietary PUFA are present. Fish fed the LO diet for 10 weeks contained the lowest amount of n‐3 HUFA in fillets among dietary treatment groups. However, feeding these fish the Schizochytrium diet for an additional 4 weeks increased the n‐3 HUFA content towards the same concentration of n‐3 HUFA found in the flesh of fish fed FO, without affecting the sensory properties of the fillets. In contrary, feeding the Schizochytrium diet for a continuous period of 14 weeks lowered overall sensory property scores. 相似文献
109.
Rochefort C Arabo A André M Poucet B Save E Rondi-Reig L 《Science (New York, N.Y.)》2011,334(6054):385-389
Spatial representation is an active process that requires complex multimodal integration from a large interacting network of cortical and subcortical structures. We sought to determine the role of cerebellar protein kinase C (PKC)-dependent plasticity in spatial navigation by recording the activity of hippocampal place cells in transgenic L7PKCI mice with selective disruption of PKC-dependent plasticity at parallel fiber-Purkinje cell synapses. Place cell properties were exclusively impaired when L7PKCI mice had to rely on self-motion cues. The behavioral consequence of such a deficit is evidenced here by selectively impaired navigation capabilities during a path integration task. Together, these results suggest that cerebellar PKC-dependent mechanisms are involved in processing self-motion signals essential to the shaping of hippocampal spatial representation. 相似文献
110.
Patrick Rehmann Andrea Gr?ne Bruno Gottstein Jürg V?llm Heinz Sager Martin Janovsky Luca N Bacciarini 《Journal of veterinary diagnostic investigation》2005,17(2):183-186
Five animals in a colony of cynomolgus monkeys (Macaca fascicularis) died or were euthanatized because of alveolar echinococcosis, during a period of 5 years. The remainder of the colony was screened for possible infection with Echinococcus multilocularis, using serology and ultrasonography. A total of 46 animals out of a group of 55 were examined. The presence of anti-Em2 antibodies analyzed with enzyme-linked immunosorbent assay was demonstrated in 3 monkeys. In 2 of these 3 monkeys, multilocular structures compatible with metacestodal cysts in the liver were identified, using ultrasonography. The presence of alveolar echinococcosis was subsequently confirmed at postmortem examination in 1 animal. The other animals are still alive. Two other monkeys were negative in the serological examination but had cystic structures in the liver, which were identified as bile duct cysts at postmortem examination in 1 animal. The other monkey is still alive. These findings suggest that serology for antibodies against the Em2 antigen may represent a useful method in identifying animals that might be infected with E. multilocularis and are therefore at risk of developing fatal alveolar echinococcosis. 相似文献