Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection.     

非亲和性稻瘟病菌对水稻的诱导抗性

为研究稻瘟病菌Magnaporthe oryzae不同菌株间的相互作用,选择与单抗性基因系水稻IRBL5-M （携带抗性基因Pi5）表现为亲和性的菌株HN52与非亲和性的菌株HN119为研究对象,将其单独或混合接种到单抗性基因系水稻IRBL5-M中,并通过荧光显微镜观察接种后水稻叶鞘的发病情况及病斑面积,测定接种后水稻内相关抗性基因OsWRKY45、OsNPR1、OsPR10、OsMAPK2的表达量以及活性氧的变化。结果显示,相较于单独接种亲和性菌株,混合接种后单抗性基因系水稻IRBL5-M病斑发病面积减少;混合接种中亲和性菌株HN52菌丝侵染能力降低,侵染菌丝细胞间扩展率显著降低73.13%;同时单抗性基因系水稻IRBL5-M中OsWRKY45、OsNPR1、OsPR10和OsMAPK2抗性基因表达量显著增加,水稻叶片中活性氧含量增加,表明在菌株混合侵染过程中,非亲和性菌株可通过激发水稻的抗性反应来降低亲和性菌株对水稻的侵染程度。     

一个调控葡萄花和果实色素形成的基因家族——MYB相关基因家族

葡萄浆果的颜色是由花色素苷的量来决定的,UFGT控制花色素苷的合成,Myb相关基因通过时空表达调节UFGT,从而使花色素苷在葡萄发育的不同阶段和不同部位表达.不同葡萄品种调节花色素苷的合成的强度和模式的差异造成了有色葡萄品种的果皮有多种颜色,白色葡萄品种果皮是由于没有花色素苷的合成.Myb相关基因家族主要有八个成员,其中MybA的三个种类VlmybA1-1,VlmybA1-2和VlmybA2以及MybB的两个种类VlmybB1-1和VlmybB1-2得到了较深入的研究.     

豆制品加工中黄浆水无害化处理技术研究

对豆制品中黄浆水进行超滤处理的工艺参数进行研究。用外加压管式膜组件对黄浆水进行了超滤试验,分析了液体分别在不同的温度、压力、流速、pH值等因素下对渗透流量和截留率的影响。通过正交试验,得到了处理黄浆水废液的最佳工况。实验结果表明,超滤处理黄浆水的最佳工况是:在压力0.15MPa下,用泵的最大流量对刚排放的黄浆水(温度55℃左右)进行超滤处理,可回收全部脂肪、75%以上的蛋白质和70%以上的总糖。回收后的浓缩物(或干燥物)可以作为饲料或经纯化后食用。     

PREDICTIVE INTELLIGENT CONTROL OF THE COLD STORAGE

The predictive intelligent control scheme is proposed, which is based on approximation theory and numerical method of Chcbyshev orthogonal polynomial. It has the practical value to the cold storge using widely electromagnetic valve and can be also applied similar systems in orther area.     

The Application of 8098 Single Chip Microcomputer in an OpticaI Fibre Pred uction Line

A winding and rowing microcomputer controller is proposed to be applied in an optical fibre preduction line. This controller consists of 8 09 8 single chip microcomputer.With trac-ing the linear velocity of d rivin g m otor,m edulating optical fibre tension with PLD and calculating layers of optical fibre. The controller automatically medulates the speed of winding and rowin8 step motors.     

THE ANALYSIS OF MECHANICAL CONSTRUCTION OF SURROUNDING ROCKS IN THE PITCHING SEAM

Defferent forms of mechanical construction, the distribution of stress, and the process of the deformation destruction in the enclosing rocks in a working face of a pitching seam are analyzed according to its geological and mining specialities.     

水稻籽粒中内源多胺及其与籽粒充实和粒重的关系

以８个水稻品种（含杂交稻组合）为材料，研究了籽粒发育期内源多胺的含量及其与籽粒充实和粒重的关系。结果表明：籽粒中Ｐｕｔ（腐胺）、Ｓｐｄ（亚精胺）和Ｓｐｍ（精胺）的含量，强势粒高于弱势糕卫粒充实好的品种（组合）高于籽粒充实差的品种（组合），尤以Ｓｐｄ和Ｓｐｍ更明显；籽闰中Ｓｐｄ和Ｓｐｍ的含量或浓度，以及Ｓｐｄ／Ｐｕｔ和Ｓｐｍ／Ｐｕｔ的比值与谷粒充实率和千粒軎于极显著正相关在花后３天喷施外源多胺（１ｍ