MECHANICAL TREATMENT FOR IMPROVING UPLAND GRAZING

The botanical changes in a wet ( Molinia ) and a drier ( Nardtts ) upland pasture that followed a single defoliation by a forage harvester are briefly described. Without fertilizer the bare and litter-covered areas were soon recolonized by the original dominants; with lime and fertilizer the recolonization was mainly by Agrostis spp., even when Agrostis was a minor element in the original sward. These changes were markedly accelerated by the forage-harvester treatment. The results of surface seeding after the forage-harvester treatment are compared with those obtained after rotary cultivation. Contrasting moisture regimes and competitive thresholds led to considerable differences in sward balance and in establishment of sown species, and seeding was successful only on the wetter site. Too rapid regeneration of undesirable remnants was prevalent, but this and other hazards are related to the uneven deposit of litter left by the forage harvester; thick, slowly-rotting accumulations inhibited the redevelopment of rapidly-growing species and germination on seeded areas.     

低钾胁迫对不同钾营养效应型大豆保护酶系统的影响

以四种钾营养效应型大豆品种(系)(铁丰31号、沈农6号、铁95068-5、GD1617)为试验材料,设置两个钾浓度处理,研究低钾胁迫对大豆整个生育期细胞保护酶系统的影响.结果表明:在低钾胁迫下,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性均有不同程度的升高.SOD的活性高峰出现时期最早,CAT次之,POD出现最晚.从敏感型来看,耐性系数高的不敏感型品种(系)CAT与POD的活性差异明显高于耐性系数低的敏感型品种(系),SOD活性差异则不甚显著.由此,本文认为大豆钾营养敏感型间的差异可以通过保护酶系统活性差异得以表现.     

基于耕地质量成果的上海市耕地占补平衡运行机制研究

在对我国耕地占补平衡问题分析的基础上,结合占补平衡耕地质量内涵和上海市耕地质量成果应用条件分析,构建以数量平衡为前提的耕地质量占补平衡运行机制。该机制框架包括耕地质量评估和监测机制、占补平衡指标储备机制、占补平衡指标跨区使用机制和占补平衡实施经济保障机制等4个方面,以为完善耕地占补平衡制度提供依据。     

基于Voronoi图的单粒播玉米产量效果的蒙特卡罗模拟

为了明确在单粒播种条件下田间出苗率对玉米产量的影响,以蒙特卡罗二项分布作缺苗模拟,以Voronoi图分配缺苗土地,通过对产量-密度方程进行数学恒等变换计算产量补偿,模拟了2种单粒播策略的玉米产量随出苗率(75%~95%)的变化。结果表明,缺苗斑数目与出苗率呈单峰曲线关系,缺苗斑大小随出苗率呈负指数下降;单粒播造成0.06%~16.78%的减产,且减产率随出苗率下降而增加;两种单粒播策略的产量大小和产量稳定性均有差异,且差异随出苗率下降而扩大。该研究推荐的玉米单粒播策略是:在千方百计提高出苗率基础上,播种的种子数目应当等于目标种植密度除以出苗率、保证出苗的植株数目达到推荐种植密度。该结论对于在玉米生产上广泛采用单粒精量播种具有重要参考价值。     

昆虫嗅觉在监测物质开发上的应用

本文对昆虫嗅觉传导信息化合物的机理在监测物质开发方面的应用前景进行了综述。首先介绍了参与昆虫嗅觉传导的多种蛋白组分、关键组分OBPs和Ors缺失后对昆虫行为的影响;其次对昆虫嗅觉及其关键组分在害虫监测仪器的开发和高效诱集物质研发上的应用进行了阐述,以期提高害虫监测水平。     

轴流泵叶轮进出口流场的测量

为了获得轴流泵内部流动的真实情况,设计了内部流动测量装置.运用五孔球形探针,对高效轴流泵在0.8Qopt,1.0Qopt和1.2Qopt工况下的叶轮进口和出口速度矢量分布、静压分布和总压分布进行了测量,并分析了不同工况下的轴面速度、环量、圆周方向分速度和压力分布等.测量结果表明：轴流泵模型进口在0.8Qopt至1.2Qopt工况范围内,流动稳定,进口轴向速度从轮毂到轮缘逐渐减小,且进口预旋很小,不同径向位置的静压基本相等.在最优工况下,轮毂与可调叶片间无间隙时,轴流泵叶轮出口基本呈现等环量流型,轴面速度呈现抛物线分布,且效率高.若轮毂侧存在间隙,间隙处的环量、轴面速度、压力和效率均明显下降.     

拟南芥霜霉病抗性分子机制研究进展

随着近年分子生物学技术的发展与应用,植物霜霉病抗性的研究有了长足的进展。本文就拟南芥抗霜霉病基因的克隆与结构分析,抗病信号传导,防卫反应和系统获得抗性,以及寄主-寄生菌共进化冲突方面进行了综述,并就今后的研究进行了展望。     

Adenosine A2A receptor agonists inhibit lipopolysaccharide-induced production of tumor necrosis factor-alpha by equine monocytes

Adenosine is an endogenous nucleoside that regulates many physiological processes by activating one or more adenosine receptor subtypes, namely A1, A2A, A2B and A3. The results of previous studies indicate that adenosine analogues inhibit lipopolysaccharide (LPS)-induced production of reactive oxygen species (ROS) by equine neutrophils primarily through activation of A2A receptors. Because peripheral blood monocytes produce cytokines that are responsible for many of the deleterious effects of LPS, the current study was performed to evaluate the effects of an array of novel adenosine receptor agonists on LPS-induced production of tumor necrosis factor-alpha (TNF-alpha), and to assess the selectively of these agonists for equine adenosine A2A over the A1 receptor. Radioligand binding studies performed with equine tissues expressing adenosine A1 and A2A receptor subtypes yielded a rank order of affinity for the equine A2A receptor of ATL307>ATL309 approximately ATL310 approximately ATL313>ATL202 approximately ATL361 approximately ATL376>ATL372>CGS21680>NECA. Co-incubation of equine peripheral blood monocytes with LPS and these agonists resulted in inhibition of TNF-alpha production with a rank order of potency that strongly correlated with their binding affinities for equine adenosine A2A receptors. Results of experiments performed with one of the adenosine receptor agonists (ATL313) and selective adenosine receptor antagonists confirmed that inhibition of LPS-induced production of TNF-alpha occurred via stimulation of A2A receptors. Although incubation of monocytes with IB-MECA, a compound purported to act as an adenosine A3 receptor agonist, reduced LPS-induced TNF-alpha production, this effect of IB-MECA was inhibited by the A2A selective antagonist ZM241385 but not by the A3 receptor antagonist MRS1220. These results indicate that the adenosine receptor subtype responsible for regulation of LPS-induced cytokine production by equine monocytes is the A2A receptor. To address the signal transduction mechanism responsible for the anti-inflammatory effects of ATL313 in equine monocytes, production of cAMP was compared in the presence and absence of either the adenosine A2A receptor antagonist ZM241385 or the adenosine A2B receptor antagonist MRS1706. In the absence of the antagonists, ATL313 increased production of cAMP; ZM241385 inhibited this effect of ATL313, whereas MRS1706 did not. Furthermore, incubation of monocytes with either the stable analogue of cAMP, dibutyryl cAMP, or forskolin, an activator of adenylyl cyclase, also inhibited LPS-induced production of TNF-alpha production by equine monocytes. Collectively, the results of the current study indicate that adenosine analogues inhibit LPS-induced production of TNF-alpha by equine monocytes primarily via activation of adenosine A2A receptors and do so in a cAMP-dependent manner. The results of this study indicate that stable adenosine analogues that are selective for adenosine A2A receptors may be suitable for development as anti-inflammatory drugs in horses.     

The influence of perfusate volume on antimicrobial concentration in synovial fluid following intravenous regional limb perfusion in the standing horse

This study investigated the influence of perfusate volume on antimicrobial concentration in synovial fluid following intravenous regional limb perfusion (IVRLP) and assessed the efficacy of low volume IVRLP. The front limbs of 9 horses were randomly assigned to 1 of 3 volume groups: 10 mL (Group 1), 30 mL (Group 2), or 60 mL (Group 3). A tourniquet was applied distal to the carpus and the limbs were perfused with 500 mg genta-micin diluted to the assigned volume via a catheter placed in the lateral palmar digital vein at the level of the proximal sesamoid bones. Synovial fluid samples were collected from the metacarpophalangeal joint at 30 minutes, followed by removal of the tourniquet. Gentamicin concentration in synovial fluid was detected using a fluorescence polarization immunoassay. There were no statistically significant differences among gentamicin concentrations in synovial fluid among perfusate volume groups. Mean gentamicin concentration in Group 1 (125.9 μg/mL) was higher than Group 2 (82.7 μg/mL) and Group 3 (56.1 μg/mL).     

一起不履行动物强制免疫义务案的分析

通过对一起不履行动物强制免疫义务案件的分析,指出同类案件在办理过程中容易出现的问题,以及的目前执法工作中存在的一些不足,针对这些问题提出了解决建议。