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排序方式: 共有113条查询结果,搜索用时 31 毫秒
81.
K Gutierrez L Siqueira ML Rigo WG Glanzner JF Oliveira PB Gonçalves 《Reproduction in domestic animals》2013,48(5):844-849
The aim of the present study was to examine the role of oxytocin (OT) in the progesterone (P4) and prostaglandins (PGs) pathway to induce oocyte meiotic resumption. Cumulus–oocyte complexes were co‐cultured with follicular hemisections for 15 h to determine the effects of different doses of OT or atosiban (ATO; oxytocin receptor antagonist) on oocyte meiotic resumption. In another experiment, we examined the effect of the interaction between P4, OT and PGs on the regulatory cascade of the oocyte meiotic resumption. Oxytocin at 1 μm was effective in inducing meiotic resumption in oocytes co‐cultured with follicular cells (84.0%), not differing from the positive control group (74.4%). Atosiban inhibited in a dose‐dependent manner the positive effect of OT on the meiotic resumption (27.6% metaphase I with 10 μm of ATO, which did not differ from the 25.5% of the negative control group). Furthermore, a third experiment showed that P4 was able to induce oocyte meiotic resumption, which was inhibited by ATO. However, the OT positive effect was not blocked by mifepristone (P4 antagonist), but was inhibited by indomethacin (a non‐selective PTGS2 inhibitor). Collectively, these data suggest a sequential role of P4, OT and PGs in the induction of oocyte meiotic resumption. 相似文献
82.
Genetic differences in susceptibility to a mixture of avian infectious bronchitis virus and Escherichia coli 总被引:1,自引:0,他引:1
Two-week-old chickens of 9 inbred and partially inbred lines of chickens were challenged intranasally with a mixed infection consisting of a pool of virulent strains of infectious bronchitis virus and a pool of pathogenic strains of Escherichia coli. 2. Wide differences in mortality were observed in the different lines, ranging from 3% in a Brown Leghorn line to 87% in White Leghorn line 7(2). 3. Experiments involving challenge with E. coli alone or virus alone suggested that this variation reflected resistance to the virus rather than to E. coli. 4. Reciprocal F1 matings suggested these differences in mortality were not attributable to maternal effects and indicated that the inheritance of resistance was fully dominant. 5. The pattern of mortality in F2 and backcross progeny of matings was compatible with the inheritance of a dominant autosomal resistance gene and showed no evidence of association with the major histocompatibility complex. 相似文献
83.
Di Fabio J Rossini LI Orbell SJ Paul G Huggins MB Malo A Silva BG Cook JK 《Avian diseases》2000,44(3):582-589
Fifteen isolations of infectious bronchitis (IB) virus were made from a total of 126 Brazilian poultry flocks of all ages that were examined. These flocks (14 chicken and 1 quail) were experiencing a variety of IB-like conditions including respiratory disease, digestive and kidney problems, and drops in egg production. One of the isolates was of the Massachusetts serotype. The remainder were examined by means of cross-neutralization tests in tracheal organ cultures and were shown to belong to at least four antigenic groups, all different from ones described previously in other countries. Some, but not all, of the flocks from which they were isolated had been vaccinated against IB with vaccines of the Massachusetts serotype. In vivo protection studies showed that the MA5 vaccine (of the Massachusetts serotype) protected well against challenge with four of these isolates, representing the different serotypes reported in this study. 相似文献
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86.
HS Stokes JM Martens A Chamings K Walder ML Berg Y Segal ATD Bennett 《Australian veterinary journal》2019,97(10):398-400
Chlamydia gallinacea is a recently described bacterial species in a genus known to infect and cause disease in animals and humans. Our report describes the identification of C. gallinacea infection in free‐range laying chickens (Gallus gallus) in Australia, and the identification of C. gallinacea infection in a parrot, a wild Australian galah (Eolophus roseicapillus). There is currently little knowledge of the effects of C. gallinacea infection on avian hosts, but it has been linked to respiratory disease in humans and could potentially cause similar disease in other species. Our report highlights the need for further study and surveillance of Chlamydia species in both wild and domestic hosts in Australia. 相似文献
87.
Qualitative and quantitative ultrasound attributes of maternal‐foetal structures in pregnant ewes
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PDA da Silva RAR Uscategui VJC Santos AR Taira RSG Mariano MGK Rodrigues APR Simões MC Maronezi ML Avante WRR Vicente MAR Feliciano 《Reproduction in domestic animals》2018,53(3):725-732
The aim of this study was to examine foetal organs and placental tissue to establish a correlation between the changes in the composition of these structures associated with their maturation and the ultrasonographic characteristics of the images. Twenty‐four pregnant ewes were included in the study. Ultrasonography assessments were performed in B‐mode, from the ninth gestational week until parturition. The lungs, liver and kidneys of foetuses and placentomes were located in transverse and longitudinal sections to evaluate the echogenicity (hypoechoic, isoechoic, hyperechoic or mixed) and echotexture (homogeneous and heterogeneous) of the tissues of interest. For quantitative evaluation of the ultrasonographic characteristics, it was performed a computerized image analysis using a commercial software (Image ProPlus®). Mean numerical pixel values (NPVs), pixel heterogeneity (standard deviation of NPVs) and minimum and maximum pixel values were measured by selecting five circular regions of interest in each assessed tissue. All evaluated tissues presented significant variations in the NPVs, except for the liver. Pulmonary NPVmean, NPVmin and NPVmax decreased gradually through gestational weeks. The renal parameters gradually decreased with the advancement of the gestational weeks until the 17th week and later stabilized. The placentome NPVmean, NPVmin and NPVmax decreased gradually over the course of weeks. The hepatic tissue did not show echogenicity and echotexture variations and presented medium echogenicity and homogeneous echotexture throughout the experimental period. It was concluded that pixels numerical evaluation of maternal‐foetal tissues was applicable and allowed the identification of quantitative ultrasonographic characteristics showing changes in echogenicity related to gestational age. 相似文献
88.
OBJECTIVES: To determine the prevalence of Toxoplasma gondii in a population of domestic cats in Melbourne. DESIGN: An ELISA assay was used to measure T gondii antibody titres in 103 cats from north-eastern Melbourne. Cats were obtained from outer suburban areas (less than 30 km from the Melbourne GPO) and from rural areas (more than 30 km from the Melbourne GPO). RESULTS: Thirty-nine percent of cats were positive for T gondii IgG. Older cats tended to have higher antibody titres. There was no significant difference in the T gondii antibody titres between males and females, or between cats living in urban areas and cats from rural areas. CONCLUSIONS: A significant proportion of cats from Melbourne have been exposed to Toxoplasma. This may have implications for the health of wildlife and humans. 相似文献
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90.
Social Capital and Entrepreneurship: Does the Relationship Hold in Deprived Urban Neighbourhoods?
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The aim of this paper is to examine the relationship between social capital and entrepreneurship in deprived urban neighbourhoods (DUNs). While there is a great deal of research on social capital, its usage and value in deprived areas is an under‐researched topic. Drawing on survey data, it is found that social capital in the form of network ties within a DUN is linked to how entrepreneurship develops in these places. The breadth and variety of network ties that individuals are able to identify as sources of advice is related to the probability of considering an entrepreneurial career, and is even more strongly related to the conversion of entrepreneurial intentions into full engagement. If entrepreneurship is to be harnessed in DUNs a starting point is the enhancement of initiatives for developing social capital incorporating local businesses, residents, and local government agencies. 相似文献