Model Prediction of the Effects of Changing Phosphorus Loads on the Everglades Protection Area

The Everglades Phosphorus and Hydrology (EPH) model was developed to simulate water movement and phosphorus transportin the Everglades Protection Area which is comprised of theEverglades National Park (ENP) and surrounding wetlands knownas the Water Conservation Areas (WCAs). Water flows from theEverglades Agricultural Area (EAA) through the WCAs intoEverglades National Park (ENP). The model is designed to represent the system as a series of cells in which water flowsfrom one cell to the next. The code allows for pumped inputsand pumped outputs of water as well as sorption and removal ofphosphorus through peat accretion. Model application involved dividing the system into twenty cellsrepresenting different segments of the WCAs. Inputs to each cellconsisted of water pumped from the EAA (where appropriate), flowfrom upgradient cells, and precipitation. Outputs included pumped outputs and flow out of each cell. Using data collectedby the South Florida Water Management District, the model wascalibrated by matching simulated and observed flows, water elevations, and phosphorus (P) concentrations for the period 1980–1988. The model was then validated for the 1988–1992 period using the same model parameters derived from the calibration process and comparing simulated and observed values.Reasonable agreement between simulated and observed values wasattained for both the calibration and validation periods. The calibrated and validated model was used to simulate the impacts on annual average total P concentrations in each cellresulting from the implementation of the management plan mandated by the Everglades Forever Act. This plan calls for the construction of six Stormwater Treatment Areas (STAs) to treat discharges from the EAA, hydrologic modifications of thesystem to promote sheet flow, and the implementation of BestManagement Practices to reduce P runoff from individual farms. In addition, the model was used to evaluate the impact of notbuilding one of the STAs (STA 3/4), and sensitivity analyseswere conducted to determine the effects of changing STA outletP concentrations throughout the system. Model results indicatethat phosphorus concentration reductions will occur in areas near EAA discharges in response to reductions in input P concentrations. However these measures will have little impacton phosphorus concentrations for 85% of the area of theWCAs and on the water entering Everglades National Park. The scenario analyses also indicate that phosphorus concentrationsthroughout most of the WCAs are similar with or without the construction of STA-3/4.     

Characterization of antibiotic-resistant bacteria in rendered animal products.

Antibiotics are used in food animal production to treat diseases and also to improve performance. Antibiotics are not used on all farms, and antibiotic resistance is occasionally found on farms that do not use antibiotics. Rendered animal protein products are often included in poultry feeds and could potentially serve as a source of antibiotic-resistant bacteria. One hundred sixty-five rendered animal protein products from cattle, poultry, and fish were aseptically collected from poultry feed mills. Fifty-five percent of the poultry meal samples had detectable levels of gram-negative bacteria ranging from 40 to 10,440 colony-forming units/g of sample. Poultry meal and meat and bone meal had the greatest number of samples with bacteria resistant to five or more antibiotics. A high percentage of feed samples (85%) contained bacteria resistant to amoxicillin, ampicillin, clavulanic acid, or cephalothin, whereas few samples contained bacteria resistant to ciprofloxacin, kanamycin, or trimethoprim/sulfamethoxazole. Acinetobacter calcoaceticus, Citrobacter freundii, and Enterobacter cloacae were the most commonly isolated antibiotic-resistant bacteria. Isolation for Salmonella was also performed, with 14% of the meat and bone meal samples containing Salmonella sp. Only one of the meat and bone meal isolates, Salmonella livingstone, was resistant to five or more antibiotics. Many of the antibiotic-resistant bacteria contained integrons, genetic elements that mediate multiple drug resistance.     

Ventral rupture of the penile tunica albuginea and urethra distal to the sigmoid flexure in a bull

A 4-year-old Limousin bull sustained a rupture of the ventral aspect of the tunica albuginea of the penis, 20 cm distal to the distal bend of the sigmoid flexure. The corpus spongiosum penis and urethra apparently ruptured at the same time.     

Swine dysentery: protection of pigs by oral and parenteral immunisation with attenuated Treponema hyodysenteriae.

An attenuated strain of Treponema hyodysenteriae was used to immunise 18 pigs in three experiments. Live attenuated spirochaetes were dosed orally and injected intra-peritoneally, and killed spirochaetes were injected intramuscularly with adjuvant. The vaccinated pigs, which developed high serum agglutination titres against T hyodysenteriae, and 18 unvaccinated litter-mates were repeatedly challenged with virulent T hyodysenteriae. Nine vaccinated pigs and 16 control pigs developed typical swine dysentery.     

Effect of unilateral orchiectomy on semen quality in bulls

Unilateral orchiectomy was performed on 9 mature mixed-breed bulls with satisfactory semen quality to study the effect of the procedure on quality of semen from the contralateral testicle. Semen was collected by electroejaculation before surgery and on alternate days for 2 weeks, then once weekly for 8 weeks. Each sampling day, progressive motility and morphologic features of spermatozoa were determined, and scrotal thermograms were taken. The percentage of normal spermatozoa decreased significantly (P less than 0.05) only on postoperative day 6. Progressive motility scores varied but at the end of the study there was no significant difference from preoperative values. Scrotal thermography revealed inflammation in the contralateral side of the scrotum, beginning 3 days after surgery, but the thermograms were normal in most bulls by 3 weeks after surgery, and all thermograms were normal by 4 weeks.     

Cell Adherence Reactions in Lungworm (Protostrongylus) Infections of the Rocky Mountain Bighorn Sheep

The immunological response of captive naturally-infected Rocky Mountain bighorn sheep to the lungworm, Protostrongylus stilesi, was examined throughout the seasonal cycle of parasite activity using a cell adherence assay for circulating antibody. The net effect of all stable serum factors on cell adherence was examined by exposure of normal cells to larvae in the presence of test serum (decomplemented-serum test). Homocytotropic antibody was examined by exposure of washed normal cells to larvae after passive sensitization with serum from infected animals (washed-cell test). Although the washed-cell test did not show a clear association with parasite activity, the net ability of sera to promote adherence showed a significant increase during periods of elevated Protostrongylus activity. Fecal counts of Muellerius, Nematorirus and coccidia were not associated with the results of either assay. Significant differences among animals were detected only for the washed-cell test when the effects of parasitism were considered.