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61.
Four hundred male chickens were selected to study the effects of pyruvate (Pyr), creatine pyruvate (CrPyr) and creatine (Cr) on the expression of hepatic mitochondrial and cytoplasm proteins associated with lipid and protein metabolism. Mitochondrial purification was accomplished using the two-step differential centrifugation and density gradient method, and the activities of organelle-specific marker enzymes were determined to assess the purity of the mitochondria. Proteins were extracted and fractionated by two-dimensional electrophoresis and the differential protein spots were assessed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. CrPyr reduced fatty acid accumulation by down-regulating adipose differentiation-related protein, inhibited ATP synthase expression, and reduced cholesteryl ester transfer protein (CETP) expression, thus reducing the levels of high density lipoprotein and triglycerol (TG) levels (thereby lowering fat and cholesterol deposition). CrPyr increased the expression of eukaryotic translation initiation factor (eIF) 2B, calreticulin (CRT) and eIF3a, thus promoting protein synthesis. CrPyr up-regulated the expression of fatty acid-binding proteins, CETP and apolipoprotein A-IV in cytoplasmic extracts, and these proteins accelerated the decomposition of fatty acids and TG, thus reducing fat deposition. In conclusion, CrPyr plays an important role in lipolysis and protein synthesis, and this effect was more pronounced than was the effect of Pyr and Cr. 相似文献
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63.
中国兽药行业的发展现状与未来方向 总被引:1,自引:0,他引:1
一、现状分析
我国的兽药行业,近20年来伴随着改革开放的步伐,取得了举世瞩目的成就,书写了一个全体行业同仁艰苦奋斗、勇于创新的壮丽诗篇.与80年代初期相比,无论从产品规格、生产规模、还是市场营销,一直到研发与经营管理队伍建设及资本运作都达到了前所未有的高度.全行业显示出巨大的生命活力和诱人的产业前景.据初步统计我国现有兽药企业约2600家,生产品种(规程)约2000~3000种,年产值约150亿元;约有经销商7万多家,年营业额约170亿元,其中销售额在1000万元以上的有220多家,销售额在500万~1000万的有1300多家(于康震2003),其中以保定冀中药业有限公司、北京保吉安集团公司等为代表的一大批技术先进,管理新颖,产品占有率高的高科技型企业正逐步斩露头角,市场份额不断扩大。 相似文献
64.
Lee YC Leu SJ Hung HC Wu HH Huang IJ Hsieh WS Chiu WT Hsieh MS Cheng TF Yang YY 《Veterinary immunology and immunopathology》2007,117(1-2):75-85
Severe acute respiratory syndrome (SARS) is a newly emergent human disease, which requires rapid diagnosis and effective therapy. Among antibody sources, immunoglobulin Y (IgY) is the major antibody found in chicken eggs and can be used as an alternative to mammalian antibodies normally used in research and immunotherapy. In this study, phage-expressing chicken monoclonal scFv antibody was chosen and characterized with phage display antibody technology. Truncated fragments of SARS-CoV spike protein were cloned in pET-21 vector and expressed in BL-21 Escherichia coli (E. coli) cells. After purification, the purity of these recombinant spike proteins was examined on SDS-PAGE and their identity verified with Western blot analysis using anti-his antibodies and sera from convalescent stage SARS-CoV-infected patients. Using these bacteria-derived proteins to immunize chickens, it was found that polyclonal IgY antibodies in the egg yolk and sera were highly reactive to the immunogens, as shown by Western blot and immunocytochemical staining analysis. A phage displaying scFv library was also established from spleen B cells of immunized chicken with 5 x 10(7) clones. After four panning cycles, the eluted phage titer showed a 10-fold increase. In sequence analysis with chicken germline gene, five phage clones reacted, with large dissimilarities of between 31 and 62%, in the complementarity-determining regions, one dominant phage 4S1 had strong binding to fragment Se-e, located between amino acid residues 456-650 of the spike protein and this particular phage had significantly strong binding to SARS-CoV-infected Vero E6 cells. Based on the results, we conclude that generating specific scFv-expressing phage binders with the phage display system can be successfully achieved and that this knowledge can be applied in clinical or academic research. 相似文献
65.
基于前海蛇口自贸区动植物检疫工作的现状和需求,对自贸区动植检快筛实验室的建设要素进行了分析和探讨,提出了以快筛快检、生物安全监测和技术交流平台为主要功能,建立自贸区动植检快筛实验室,并在实验室的建立标准、质量控制和生物安全等方面提出了初步建议,以期为自贸区动植检快筛实验室的建设提供借鉴。 相似文献
66.
山羊传染性胸膜肺炎又称山羊支原体肺炎,临床上以高热、咳嗽、肺和胸膜发生浆液性和纤维蛋白性炎症为特点,传播快,呈急性或慢性经过,发病率及病死率很高. 相似文献
67.
Jiarong Xu Dongyan Huang Jiaping Xu Shichao Liu Huixing Lin Haodan Zhu Bao Liu Wen Chen Chengping Lu 《Veterinary microbiology》2013
The recombinant swine poxvirus rSPV/H3-2A-H1 co-expressing HA1 genes of H3N2 and H1N1 subtype SIV has been constructed and identified. Inoculations of rSPV/H3-2A-H1 yielded ELISA and neutralization antibodies against SIV H1N1 and H3N2, and elicited potent H1N1 and H3N2 SIV-specific INF-γ response from T-lymphocytes in mice and pigs in this study. Complete protection against SIV H1N1 or H3N2 challenge in pigs was observed. 相似文献
68.
69.
本文采用化学分析及仪器分析两种测试方式,对饲料样品的粗蛋白质含量进行分析检验,旨在探讨两种分析测试方法的测量误差,经统计分析,两种测试方法的分析结果无显著差异。同化学分析法相比较,FossTecator2300凯氏定氮仪具有自动化程度高、分析速度快、分析数据准确等优势。 相似文献
70.