Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens

Four hundred male chickens were selected to study the effects of pyruvate (Pyr), creatine pyruvate (CrPyr) and creatine (Cr) on the expression of hepatic mitochondrial and cytoplasm proteins associated with lipid and protein metabolism. Mitochondrial purification was accomplished using the two-step differential centrifugation and density gradient method, and the activities of organelle-specific marker enzymes were determined to assess the purity of the mitochondria. Proteins were extracted and fractionated by two-dimensional electrophoresis and the differential protein spots were assessed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. CrPyr reduced fatty acid accumulation by down-regulating adipose differentiation-related protein, inhibited ATP synthase expression, and reduced cholesteryl ester transfer protein (CETP) expression, thus reducing the levels of high density lipoprotein and triglycerol (TG) levels (thereby lowering fat and cholesterol deposition). CrPyr increased the expression of eukaryotic translation initiation factor (eIF) 2B, calreticulin (CRT) and eIF3a, thus promoting protein synthesis. CrPyr up-regulated the expression of fatty acid-binding proteins, CETP and apolipoprotein A-IV in cytoplasmic extracts, and these proteins accelerated the decomposition of fatty acids and TG, thus reducing fat deposition. In conclusion, CrPyr plays an important role in lipolysis and protein synthesis, and this effect was more pronounced than was the effect of Pyr and Cr.     

一例剖腹产母犬及仔犬的护理

一头4岁龄史宾格母犬于2005年3月12日发情,3月21日和3月23日交配两次。该母犬5月21日上午出现产前征兆,扒窝,烦躁,于17点45分开始产第1头仔犬,至当晚20点50分产下第4头仔犬后,由于第5头仔犬胎位不正,至5月22日上午9点30分由兽医人工助产,仔犬死亡。10点10分产下第6头仔犬后,第7头仔犬又出现胎位不正。此时母犬体力出现下降,呼吸急促,兽医决定实施剖腹产,手术于17点20分至19点10分进行,剖腹后拿出4头仔犬,其中2头死亡,2头存活。     

中国兽药行业的发展现状与未来方向

一、现状分析 我国的兽药行业,近20年来伴随着改革开放的步伐,取得了举世瞩目的成就,书写了一个全体行业同仁艰苦奋斗、勇于创新的壮丽诗篇.与80年代初期相比,无论从产品规格、生产规模、还是市场营销,一直到研发与经营管理队伍建设及资本运作都达到了前所未有的高度.全行业显示出巨大的生命活力和诱人的产业前景.据初步统计我国现有兽药企业约2600家,生产品种（规程）约2000～3000种,年产值约150亿元;约有经销商7万多家,年营业额约170亿元,其中销售额在1000万元以上的有220多家,销售额在500万～1000万的有1300多家（于康震2003）,其中以保定冀中药业有限公司、北京保吉安集团公司等为代表的一大批技术先进,管理新颖,产品占有率高的高科技型企业正逐步斩露头角,市场份额不断扩大。     

A dominant antigenic epitope on SARS-CoV spike protein identified by an avian single-chain variable fragment (scFv)-expressing phage

Severe acute respiratory syndrome (SARS) is a newly emergent human disease, which requires rapid diagnosis and effective therapy. Among antibody sources, immunoglobulin Y (IgY) is the major antibody found in chicken eggs and can be used as an alternative to mammalian antibodies normally used in research and immunotherapy. In this study, phage-expressing chicken monoclonal scFv antibody was chosen and characterized with phage display antibody technology. Truncated fragments of SARS-CoV spike protein were cloned in pET-21 vector and expressed in BL-21 Escherichia coli (E. coli) cells. After purification, the purity of these recombinant spike proteins was examined on SDS-PAGE and their identity verified with Western blot analysis using anti-his antibodies and sera from convalescent stage SARS-CoV-infected patients. Using these bacteria-derived proteins to immunize chickens, it was found that polyclonal IgY antibodies in the egg yolk and sera were highly reactive to the immunogens, as shown by Western blot and immunocytochemical staining analysis. A phage displaying scFv library was also established from spleen B cells of immunized chicken with 5 x 10(7) clones. After four panning cycles, the eluted phage titer showed a 10-fold increase. In sequence analysis with chicken germline gene, five phage clones reacted, with large dissimilarities of between 31 and 62%, in the complementarity-determining regions, one dominant phage 4S1 had strong binding to fragment Se-e, located between amino acid residues 456-650 of the spike protein and this particular phage had significantly strong binding to SARS-CoV-infected Vero E6 cells. Based on the results, we conclude that generating specific scFv-expressing phage binders with the phage display system can be successfully achieved and that this knowledge can be applied in clinical or academic research.     

自贸区动植检快筛实验室建设要素分析

基于前海蛇口自贸区动植物检疫工作的现状和需求,对自贸区动植检快筛实验室的建设要素进行了分析和探讨,提出了以快筛快检、生物安全监测和技术交流平台为主要功能,建立自贸区动植检快筛实验室,并在实验室的建立标准、质量控制和生物安全等方面提出了初步建议,以期为自贸区动植检快筛实验室的建设提供借鉴。     

山羊传染性胸膜肺炎的诊治

山羊传染性胸膜肺炎又称山羊支原体肺炎,临床上以高热、咳嗽、肺和胸膜发生浆液性和纤维蛋白性炎症为特点,传播快,呈急性或慢性经过,发病率及病死率很高.     

Immune responses and protective efficacy of a recombinant swinepox virus co-expressing HA1 genes of H3N2 and H1N1 swine influenza virus in mice and pigs

The recombinant swine poxvirus rSPV/H3-2A-H1 co-expressing HA1 genes of H3N2 and H1N1 subtype SIV has been constructed and identified. Inoculations of rSPV/H3-2A-H1 yielded ELISA and neutralization antibodies against SIV H1N1 and H3N2, and elicited potent H1N1 and H3N2 SIV-specific INF-γ response from T-lymphocytes in mice and pigs in this study. Complete protection against SIV H1N1 or H3N2 challenge in pigs was observed.     

安卡纯系肉鸡六个微卫星位点遗传多样性研究

安卡肉鸡是生长速度最快的有色羽肉鸡之一,具有适应性强、耐应激、饲料报酬高等特点。为了了解其群体遗传结构,实验利用6个微卫星标记对3个品系共150只个体进行遗传多样性检测。结果表明3个品系都具有较高的多态性,反映了群体基本的遗传特征,为安卡肉鸡的育种和利用提供了基本的研究数据。     

饲料中粗蛋白质的仪器分析

本文采用化学分析及仪器分析两种测试方式,对饲料样品的粗蛋白质含量进行分析检验,旨在探讨两种分析测试方法的测量误差,经统计分析,两种测试方法的分析结果无显著差异。同化学分析法相比较,FossTecator2300凯氏定氮仪具有自动化程度高、分析速度快、分析数据准确等优势。     

益生菌发酵饲料对猪的生长和肉质的影响

应用益生菌发酵饲料喂猪,能提高饲料中粗蛋白含量,明显降低猪粪臭味,促进生长、降低料肉比和提高猪肉品质。