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41.
Huang Y Yang Z Putaporntip C Miao M Wei H Zou C Jongwutiwes S Cui L 《Veterinary parasitology》2011,176(1):9-15
Southeast Asian macaques are hosts of a number of Plasmodium infections, some of which are transmittable to humans. During examination of blood films of five wild-caught long-tailed macaques Macaca fascicularis from South China, malaria infection was detected in one of the monkeys. In order to isolate this parasite for identification and characterization, we experimentally passed this parasite through both Assamese (M. assamensis) and rhesus (M. mulatta) monkeys by intravenous injection of infected blood. This parasite morphologically resembled Plasmodium inui, and had a typical 72 h quartan periodicity. This parasite was infective to Anopheles dirus mosquitoes, and salivary gland sporozoites appeared 13 days post feeding. Feeding by 20 infected An. dirus mosquitoes on another Assamese monkey produced infection with a prepatent period of 8 days. Molecular analysis of the small subunit rRNA genes and the mitochondrial genome confirmed this parasite as an isolate of P. inui. In spleen-intact macaques, the infection had a protracted duration with parasites being detected during the rearing of the infected monkeys for over two years. In summary, this study identified a P. inui isolate and successfully passed this parasite through Assamese monkeys by both intravenous inoculation and mosquito transmission. 相似文献
42.
Pei-Pei Zhang Jing-Jing Wang Chong-Yang Li Hai-sheng Hao Hao-Yu Wang Wei-Hua Du Yun-Wei Pang Shan-Jiang Zhao Hui-Ying Zou Tong Hao Sha Yang Wei-Min Ruan Jin-Ming Huang Peng-cheng Jiao Hua-Bin Zhu Xue-Ming Zhao 《Reproduction in domestic animals》2020,55(9):1103-1114
The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10–3, 1 × 10–4, 1 × 10–5, 1 × 10–6 M) or Lyc (0, 1 × 10–4, 1 × 10–5, 1 × 10–6, 1 × 10–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 × 10–3 M VC or 1 × 10–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10–4 M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 × 10–3 M VC or 1 × 10–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure. 相似文献
43.
尿素中毒在兽医临床上较多见。笔者曾治疗6例病牛,皆因误食多量尿素后发病,经及时抢救均痊愈,现将解毒方法总结如下。 相似文献
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45.
Deng Y Sun CQ Cao SJ Lin T Yuan SS Zhang HB Zhai SL Huang L Shan TL Zheng H Wen XT Tong GZ 《Veterinary microbiology》2012,159(3-4):490-493
Nested RT-PCR was used to investigate bovine viral diarrhea virus in 511 specimens collected from Chinese pigs exhibiting clinical symptoms between 2007 and 2010. Of these, 137 samples were BVDV-positive and the BVDV prevalence rate was 23.1% (9/39) in 2007, 27.7% (44/159) in 2008, 33.6% (34/101) in 2009, and 23.6% (50/212) in 2010. Twenty of 137 BVDV-positive samples were used for further genetic analysis of the 5'-UTR. Phylogenetic analysis revealed that they were BVDV-1 and subtyped into BVDV-1a, BVDV-1b, BVDV-1m, BVDV-1o and an unknown subgenotype. This study showed that BVDVs were highly prevalent in Chinese pig herds and appropriate measures should be taken to control BVDV prevalence in pig herds. 相似文献
46.
香菇多糖是从香菇中提取分离的一种活性多糖,具有较强的免疫活性作用。本文综述了香菇多糖的免疫调节作用及其在家禽中的应用效果。 相似文献
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49.
屠宰企业的非洲猪瘟自检对切断病毒传播链条、打好非洲猪瘟攻坚战有着重要作用。为摸清四川省宜宾市屠宰企业的非洲猪瘟检测水平,对全市10个区县83家屠宰企业组织开展了非洲猪瘟检测能力比对工作。结果显示:屠宰企业检测人员偏少、岗位工作时间短、文化程度不高;不同区县、不同类别、不同组合的检测结果存在差异,有的差异较大。总的检测敏感性为49.72%,特异性为87.41%,准确率为66.56%。结果表明:屠宰企业的检测能力较为一般,监测风险预警效果不甚理想,有待加强。建议从保障人员队伍和加强操作培训两方面入手,提高屠宰企业非洲猪瘟检测能力。 相似文献
50.
为评估罗非鱼湖病毒(tilapia lake virus,TiLV)跨境传入风险,按照OIE传入风险评估框架,从危害识别、风险评估及风险管理3个方面,开展了TiLV跨境传入风险分析,并提出了相应的风险控制措施。分析认为:活的敏感鱼类(包括亲鱼、鱼卵和鱼苗),用做鲜活饵料的鱼类(野杂鱼)及冰冻和冰鲜的敏感鱼类(包括去除内脏的鱼肉)TiLV传入风险较高,而敏感鱼加工类产品、非敏感鱼类(包括活的和其他形式的产品)及运输活鱼的水、包装、运输工具和用具等传入风险较低。建议不从疫区进口高风险产品,对于低风险产品可以自由贸易,而对运输活鱼的水、包装、运输工具和用具等要进行强制常规消毒。 相似文献