提高高校青年教师教学能力的途径

为了提高青年教师教学能力,对黑龙江八一农垦大学青年教师教学能力现状进行了分析,通过培养教师自主学习能力、完善教师培养培训体系、建立科学的教学考评制度、加强师风师德建设和教师交流多元化等途径,使青年教师的教学能力和水平明显提高。     

Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice()

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.     

猪结合珠蛋白基因的克隆、原核表达及单克隆抗体制备

本研究的目的在于利用重组猪结合珠蛋白(Haptoglobin,Hp)制备特异性单克隆抗体.以梅山猪肝脏总RNA为模板,利用RT-PCR方法扩增猪hp基因,将其克隆到原核表达栽体pGEX-KG和pET-32a上,转化至E.coli BL21中,IPTG诱导下高效表达重组GST-Hp和HIS-Hp蛋白.用纯化的重组GST-Hp免疫BALB/c小鼠,用纯化的HIS-Hp作为包被抗原进行间接ELISA检测,通过淋巴细胞杂交瘤技术筛选阳性细胞株,制备单克隆抗体.结果表明筛选到2株高效分泌抗猪Hp单克隆抗体的杂交瘤细胞株,命名为3B4和3C8.Western-blot和间接ELISA检测均表明制备的单克隆抗体具有良好的特异性.     

蛋鸡饲料中添加植酸酶对蛋鸡生产性能的影响

摘要： 选择40周龄健康的海兰褐壳蛋鸡1200只,随机分为3组,设1个对照组和2个试验组。2个试验组饲喂在对照组基础上分别添加300、600U/kg植酸酶的日粮,其营养标准相对对照组分别降低了0.1%、0.2%的有效磷,研究产蛋鸡饲料中添加植酸酶来替代部分磷酸氢钙对产蛋鸡生产性能、蛋壳厚度、鸡蛋品质和饲料成本的影响。试验结果表明：产蛋鸡饲料中添加300U/kg植酸酶替代0.1%有效磷与对照组相比,产蛋率提高了1.8%,蛋重、蛋壳厚度、鸡蛋品质(哈氏单位) 、采食量统计上差异不显著（P>0.05）;添加600U/kg植酸酶替代0.2%有效磷与对照组相比,产蛋率提高了1.7%,但蛋壳厚度降低了2.8%,蛋重、采食量、鸡蛋品质(哈氏单位)统计上差异不显著（P>0.05）。     

Hemodynamic changes of abdominal organs in the early stage of septic mice induced by cecal ligation and puncture

AIM: To characterize the hemoperfusion of abdominal organs in the early stage of sepsis in mice. METHODS: Health male Kunming mice were used in the study (n=100). The techniques of 2D, M-mode and pulse-wave Doppler were applied to evaluate the systolic functions of the heart and the blood flow of abdominal aorta, right renal artery and portal vein before cecal ligation and puncture (CLP) as the baseline and at the time points of 12 h, 24 h, 36 h, 48 h and 60 h after CLP. The mice survived for 7 d were considered as survivals. All data were compared with the baseline values.RESULTS: The cardiac output of the CLP mice remained in normal or hyperdynamic levels in the early stage of sepsis. Compensatory responses of systolic functions were observed. The levels of blood flow in abdominal aorta were increased first and then decreased. Resistent index (RI) and pulsatility index (PI) of abdominal aorta began to increase at the time point of 24 h. Blood flow of right renal artery showed a significant decline from the beginning to the end of our observation. No significant difference of the right arteriorenal RI and PI was observed. Portal venous flow increased significantly at 12 h, and decreased at 24 h after CLP. Congestion index of the portal vein was distinctly increased from 12 h to the end of the observation. CONCLUSION: The hemodynamics of abdominal organs in early stage of septic mice shows specific changes, indicating an important role in evaluating the mechanism of sepsis.     

小贯小绿叶蝉与其主要天敌空间关系研究方法的比较

天敌与目标害虫空间关系密切与否,直接关系到天敌对目标害虫的控制利用。为了选择较为理想的天敌与害虫之间空间关系的研究方法,本文用地统计学法、模糊相似优先比法,扩散系数法、空间生态位重叠指数法和聚块样方方差分析法研究‘黄山大叶种’茶园和‘农抗早’茶园天敌与小贯小绿叶蝉Empoasca onukii 空间关系密切程度,以地统计学法的结果为标准,以其前3位天敌粽管巢蛛Clubiona japonicola、斜纹猫蛛Oxyopes sertatus和鳞纹肖蛸Tetragnatha squamata的位次之和为据,根据位次差别评论5种研究方法研究结果的一致性。聚块样方方差分析方法研究结果与地统计学方法的结果差别较小,其次是扩散系数法和模糊相似优先比法,空间生态位重叠指数法研究结果与地统计学方法的结果差别较大。按照天敌与小贯小绿叶蝉空间关系密切程度位次分析,粽管巢蛛、斜纹猫蛛和鳞纹肖蛸是与小贯小绿叶蝉空间关系密切的前3种天敌。本文是对多种研究方法研究结果一致性进行比较的一种尝试,为分析天敌与害虫空间关系选择研究方法提供参考。     

晋西黄土区主要造林树种耗水量测算与分析

依据水量平衡原理,采用盆栽试验分别测算了晋西黄土区主要造林树种侧柏、刺槐、杏树和梨树在其生长季(4～10月)成熟单株树木的耗水量,并根据林地土壤水分动态及土壤水分特征曲线标定的各树种无效水界值,分析了各树种土壤水分供耗特点及其有效性.结果表明:①2002贫水年生长季降水量430.7 mm,试验树种同期耗水量约为430～470 mm,树木供耗失衡;2003丰水年降水量870.2 mm,耗水量约为450～510 mm,但降雨分配不均,5月和10月树木供耗也略有失衡.②不同树种年内土壤含水量变化趋势相近,而同月耗水量差异较大,同一树种不同月耗水量差异也较大,丰水年各试验地土壤水分状况要好于贫水年.③侧柏、刺槐、杏树和梨树无效水界值为8.0%,8.4%,9.4%和10.9%,侧柏较其它树种利用水分能力最强.贫水年林外单株树木土壤含水量在一段时间内低于对应树种的无效水界值,影响树木正常生长.单株树木依靠冬季和次年春季降水补充,生长季初期都能恢复到速效水水平.     

上海郊区水稻精准施肥效益研究

以沪郊10.7万hm2优质粮田为研究区,在地理信息平台ARCGIS上,利用GPS定位网格化采集土壤样品2 817个,建立12个水稻高产示范基地。试验结果表明,应用土壤快速测定方法为当季水稻进行推荐施肥,水稻平均增产31.5 kg/666.7 m2,增幅为6.04%。     

胶体金试纸条检测H9亚型禽流感病毒的研究

对禽流感病毒H9亚型血凝素单克隆抗体4C4进行胶体金标记,喷涂于玻璃纤维上制成金标垫,鸡抗AIVIgG和羊抗鼠IgG分别包被于硝酸纤维素（NC）膜上作为检测线和质控线,制作禽流感H9亚型免疫层析检测试纸条。验证结果表明,试纸条操作简单,肉眼于10min内可判定结果,对H9亚型禽流感病毒及其标准血凝抗原具有高度特异性,与其它亚型禽流感病毒标准抗原及其它禽类病毒没有交叉反应,检测灵敏度比HA试验高4倍以上。试纸条在室温保存6个月、4℃保存12个月,其特异性及灵敏度没有明显变化。对实验室送检及临床采集的共311份喉头拭子进行检测,结果与本室研制的禽流感ELISA试剂盒总符合率为92％,随机选取10份阳性样品经病毒分离证实无一误检。结果证明,本研究建立的H9亚型禽流感病毒免疫层析检测方法具有特异、敏感、稳定、操作简单快捷等特点,适合用于H9亚型禽流感病毒的现场检测及鉴别诊断。     

氮、磷输入对黄河口潮滩湿地不同生境下碱蓬种子萌发与幼苗生长的影响

 ２０１０年１０－１１月,在人工气候箱控制条件下研究了不同氮（Ｎ）、磷（Ｐ）浓度对黄河口潮滩湿地不同生境条件下碱蓬种子萌发和幼苗生长的影响。结果表明,中潮滩碱蓬（ＪＰ１）和低潮滩碱蓬（ＪＰ２）的出芽率均随盐分的升高而降低;相同盐分条件下ＪＰ１的出芽率均高于ＪＰ２,原因可能与二者生长节律有关;高盐分条件下,ＪＰ１和ＪＰ２种子的萌发率、根重比及根冠比均在高Ｎ 条件下取得最大值,分别为２０．６％和２．０％,０．０６和０．０７,０．１０和０．１１,说明高Ｎ 可缓解盐分胁迫对碱蓬的毒害作用。Ｐ对ＪＰ１、ＪＰ２ 种子萌发和幼苗生长的影响比较复杂。在无盐分胁迫下,Ｐ对二者根重比、茎重比、叶重比和根冠比的影响几乎无差别;在中等盐分胁迫下（３００ ｍｍｏｌ／Ｌ）,不同Ｐ 浓度对二者幼苗生长的影响相近,均表现为中等Ｐ 浓度不利于根、茎生长,但有利于叶生长,且根冠比均在最大Ｐ 浓度（１０ｍｍｏｌ／Ｌ）时最大（０．１０,０．１８）;在高盐分胁迫下（６００ ｍｍｏｌ／Ｌ）,ＪＰ１ 的根重比、根冠比随Ｐ 浓度的增加而升高,叶重比随Ｐ浓度的增加而降低,ＪＰ２的生长状况正好与之相反。总的来说,Ｎ、Ｐ 浓度对不同生境下碱蓬种子萌发及幼苗生长存在影响,且整体表现在高盐分胁迫条件下,高Ｎ 浓度或低Ｐ 浓度有助于二者种子萌发和幼苗生长。加之近年来黄河口营养盐呈增加趋势,且主要污染物为无机氮,Ｐ含量相对较低,由此可见黄河水的输入对黄河口潮滩碱蓬退化区的恢复具有有利的作用。