Pathogenicity of Campylobacter jejuni for turkeys and chickens.

A Campylobacter jejuni isolate obtained from a turkey liver, designated C101, and a C. jejuni isolate obtained from the feces of a chicken, designated C111, were used to inoculate their respective hosts. Isolate C101 depressed weight gain by 20% when inoculated into newly hatched poults or 4-day-old poults. It also caused death, hepatic necrosis, and generalized hemorrhages in turkey embryos. The chicken-derived isolate, C111, did not reduce weight gain in newly hatched chicks, but it did induce mortality in chicken embryos. The supernatant of the cultures of both C. jejuni isolates also caused mortality in embryos.     

鸡大肠杆菌病中草药佐剂多价灭活苗的研制——Ⅰ、疫苗生产工艺

采用鸡大肠杆菌多发病型菌株以及地方病型菌株作为制苗用菌种，保证了疫苗既具有一定的广谱性，又具有一定的特异性；采用液体通气培养方法，在一定程度上提高了菌数；采用中草药蜂胶作为佐剂不但可有效地提高机体的免疫功能，同时兼具有治疗，保健和促生长作用，另外蜂胶还可以增大抗原表面积，延长抗原在组织的贮存时间，可持久地释放抗原，保证了机体持续产生高效价抗体。     

Susceptibility to onion-induced hemolysis in dogs with hereditary high erythrocyte reduced glutathione and potassium concentrations.

The hemolytic effect of onion consumption in dogs with hereditary high erythrocyte reduced glutathione and potassium concentrations (designated HK dogs) was compared with that in clinically normal dogs. Twelve hours after oral administration of boiled onions (200 g/dog), hemoglobin concentration decreased to 84.4% of the initial value in HK dogs; it decreased only to 90.5% in clinically normal dogs. At 24 hours, methemoglobin concentration was significantly (P less than 0.05) higher in HK dogs than in clinically normal dogs. The concentration of erythrocyte oxidized glutathione increased about fivefold at 10 hours in HK dogs, whereas it did not change during the experimental period in clinically normal dogs. In addition, at 12 hours, the proportion of erythrocytes containing Heinz bodies increased to 24.4% in HK dogs, but increased only to 1.2% in clinically normal dogs. Thus, results indicated that HK dogs were more susceptible to the oxidant action of onions than were clinically normal dogs.     

Spontaneous regression of bovine cutaneous leukosis

Biopsies of skin from a 2-year-old heifer with spontaneously regressing dermal leukosis were examined. The heifer was not infected with bovine leukemia virus and was negative for tumor-associated antigens of enzootic bovine lymphosarcoma. By hematological standards for bovine leukemia, the heifer was positive at about 60 days post-occurrence of the disease (POD). At 53 days POD, lymphoblastic neoplastic cells in the dermis reacted with anti-T lymphocyte monoclonal antibody by the avidin biotin peroxidase complex method. The cells had intracytoplasmic clustered dense bodies under electron microscopy. From 53 to 83 days POD, figures of the transepithelial elimination (TE) against neoplastic cells and perivascular infiltration of small lymphocytes were in the dermis. Small lymphocytes reacted with anti-T lymphocyte monoclonal antibody. At necropsy there were no neoplastic lesions; there were flat lymph node-like tissues in the subcutis. Many germinal centers were seen in the lymphatic organs. Blood lymphocytes at 46 days POD were stimulated by phytohemagglutinin-P and concanavalin A. Sera, taken until 75 days POD and at necropsy, showed an inhibitory effect on mitogen-induced blastogenesis of normal bovine lymphocytes. These results suggested the existence of a spontaneous regressive mechanism against neoplastic lesions by TE and tumor immunity.     

Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.     

APPLICATION AND EFFECTS OF RARE-EARTH ON SEEDLINGS

In order to improve the quality of seedlings and economic benefits,rare--earth experiments were carried out on eleven seedling-age-types of four silvicultral treespecies.Some indexes such as the increment,biomass and chlorophyl content weredetermined.The results showed that rare-earth can improve the growth in height anddiameter,increase the bioprductivity and increase the mean benefit by887766.29 yuan/hm~2.It is an effective method to improve the quality of seedlings for the characters such as lowcost and easy grasping.And the method can be widely used in seedling.     

猴头菌新菌株宁猴6号的选育初报

采用多孢分离技术产生多个新组合 ,经驯化筛选获得猴头新菌株宁猴 6号 ,宁猴 6号为适合用针叶林木屑物料栽培的新菌株。生物学特性表明 :猴头菌宁猴 6号菌丝生长适温 2 5～ 2 8℃ ,子实体生长适温 15～2 0℃ ,菌丝生长期短 ,36～ 4 0d长满瓶底 ,栽培袋在常温下培养 4 3～ 5 0d菌丝满袋。室内栽培生物学效率达5 6 .0 0 % ,室外栽培生物学效率达 6 1.4 0 % ,子实体中型匀称近球型 ,柄短或无柄 ,刺长 0 .9～ 2 .2cm。宁猴 6号不仅适于阔叶木屑栽培 ,最突出的是适应针叶林木屑栽培