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191.
Release and activity of allelochemicals from allelopathic rice seedlings 总被引:11,自引:0,他引:11
Kong C Liang W Xu X Hu F Wang P Jiang Y 《Journal of agricultural and food chemistry》2004,52(10):2861-2865
3-Isopropyl-5-acetoxycyclohexene-2-one-1 (1), momilactone B (2), and 5,7,4'-trihydroxy-3',5'-dimethoxyflavone (3) were isolated and identified from an allelopathic rice accession PI312777. These three compounds at low concentrations could inhibit the growth of weeds Echinochloa crusgalli and Cyperus difformis associated with rice, especially mixtures of the compounds had stronger inhibitory activity than did individual compounds. Studies with hydroponic culture, continuous root exudates trapping system (CRETS), and direct resin adsorption methods showed that a total of 7.6 n moles 1, 2, and 3 were exuded from living roots of each seedling into the environment at 10 days after seedlings were transplanted. Furthermore, 1, 2, and 3 were found in the soil growing PI312777 seedlings at day 15 after seedlings emergence and reached a total of 39.5 microg/g soil at day 30. The results indicated that PI 312777 seedlings could release sufficient quantities of 1, 2, and 3 into the environment to act as allelochemicals inhibiting the growth of associated weeds. Investigations on the distribution of 1, 2, and 3 in PI 312777 plant, and its root exudates showed that the levels of 1, 2, and 3 were significantly higher in the shoots and root exudates than in the roots, and only trace 1 was observed in the roots. The results suggest that the roots of rice seedlings are not major site of synthesis or accumulation 1, 2, and 3, but a pathway for their release into the environment. The levels of 1, 2, and 3 in the root exudates were over 2-folds higher under direct resin adsorption than under hydroponic culture and CRETS, and hence, it is the preferred method to collect and identify active allelochemicals in rice exudates in future studies on rice allelopathy. 相似文献
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Quantification of the group B soyasaponins by high-performance liquid chromatography 总被引:5,自引:0,他引:5
High-performance liquid chromatographic methods were developed for the isolation and quantitative determination of the group B soyasaponins, including 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated soyasaponins alphag, betag, and betaa, and their non-DDMP counterparts, soyasaponins V, I, and II, respectively, with formononetin used as the internal standard. The limits of quantification for soy products were 0.11-4.86 micromol/g. The within-day and between-days assay coefficients of variation were <9.8 and < 14.3%, respectively. The group B soyasaponin concentrations in 46 soybean varieties ranged from 2.50 to 5.85 micromol/g. Soy ingredients (soybean flour, toasted soy hypocotyls, soy protein isolates, textured vegetable protein, soy protein concentrates, and Novasoy) and soy foods (commercial soy milk, tofu, and tempeh) contained the group B soyasaponins from 0.20 to 114.02 micromol/g. There was no apparent correlation between isoflavone and soyasaponin concentrations in the soy products examined. 相似文献
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植物低聚糖提取和生物活性鉴定 总被引:1,自引:0,他引:1
应用酶解法从小麦细胞壁中提取出低聚糖。经过活性鉴定,具有诱导大豆和小麦抗毒素产生,特别是从小麦代谢、细胞、植株等水平上改善该作物体内防御功能等生物作用。水解酶的种类、纯度、酶活值与所降解低聚糖的活性程度密切相关;高纯度、高酶活单位的水解酶提取的低聚糖,具有较强的生物功能。对来源不同的低聚糖进行了活性差异的比较,同时还讨论了其它因素——诸如小麦细胞的生理状态,低聚糖保存条件等对低聚糖活性的影响。 相似文献
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农业保护性耕作技术是当今国际上先进的农业生产技术.实施以残茬覆盖和免耕、少耕为主要内容的机械化保护性耕作,可以减少地表径流,避免土壤流失,降低土壤风蚀,增加土壤有机质含量,改善耕地肥力及理化性状,是实现保土、保水、保肥和保持生态环境的重要措施,是旱作农业实现可持续发展的重要途径. 相似文献