酶法提取椰子蛋白质及其对亚基的影响

研究酶制剂的种类、用量、提取温度、pH、料液比等因素对椰子蛋白质提取率的影响,通过正交试验研究最佳的提取条件。得到的椰子蛋白质进行SDS-PAGE分析,研究酶法提取对椰子蛋白质亚基组成的影响。结果表明,添加质量分数0.3%的风味蛋白酶的提取效果最好,各因素对提取率影响的次序为:料液比>pH>温度>提取时间,其中,料液比和pH对提取率的影响达到了显著水平;最佳工艺参数为:酶质量分数0.3%、50℃、pH8.5、料液比1∶15和时间2h,在此条件下,椰子蛋白质的提取率为78.28%。SDS-PAGE分析表明,风味蛋白酶对椰子蛋白的酶解作用十分显著,与缓冲溶液提取的椰子蛋白质相比,酶法提取的椰子蛋白质中小分子质量亚基含量很高。     

中国野生葡萄醛脱氢酶基因在拟南芥中的过量表达

将质粒pWR306中包含ED35s启动子、Omega元件及TNOS终止子的一段核苷酸序列定向克隆到质粒pCAMBIA1300,构建了植物中间表达载体pWR-II;将重组质粒pGEM-T-ALDH的醛脱氢酶(ALDH)基因片段定向克隆到pWR-II,构建了葡萄ALDH基因的植物过量表达载体pWR-II-ALDH。采用改进冻融法将其导入农杆菌EHA105,采用花序浸蘸法转化拟南芥,获得了潮霉素抗性的转化拟南芥植株。PCR检测证明外源基因已整合进拟南芥基因组,Real-timePCR结果表明ALDH基因在转化植株的表达量远高于野生种。转化植株和野生植株在形态上有一定的差异,说明葡萄ALDH基因在拟南芥中表达对其生长发育有一定影响。     

荔枝的功能及活性成分研究现状

自古以来,中国就有荔枝各组织保健医疗功能的记载。现代医学、营养学、植物化学和药理学为荔枝的医疗和保健功能提供了坚实的证据,其功能活性成分正不断被揭示。对荔枝果肉抗氧化、"上火"和致敏原,果皮抗氧化和抗癌功效,核抗癌、抗病毒、降血糖、调血脂等功能和相关成分研究现状进行了介绍。     

桃花源桃花品种资源及其特殊种质

桃花源现有桃花品种25个,具有花期较早、花期较长、花色淡雅、色调柔和、花型活泼等特点。其中,‘春蕾’、‘单二乔’、‘碗寿粉’为首次报道品种;‘单红’、‘单二乔’雌蕊败育,需冷量极低,流胶病抗性强,具有稳定的秋花特性,是早花、抗流胶病品种选育的良好亲本。     

第8届国际甜椒圃地试验报告

从6个方面对参试品种进行了比较分析,结论认为：除ISPN8#3在陕西岐山试验地表现差以外,其余品种（系）均表现优良。     

沼气气肥对番茄产量和品质的影响

通过在温室大棚种植番茄增施CO2沼气肥对大棚番茄产量和品质影响研究的结果表明,施用CO2沼气肥可增加温室大棚番茄产量,改善果实品质.当温室的CO2浓度在1500 uL*L-1,每天施肥3小时,番茄小区的产量、单果重、单株果数最高,小区产量比对照组提高43%,单果重比对照组提高103%,单株果数比对照增加52%.     

Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree

To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (Δ_L) at different growth stages and fruit stable carbon isotope discrimination (Δ_F) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on Δ_L at different growth stages and Δ_F at fruit maturation stage. As compared with full irrigation, the average Δ_L at different growth stages and Δ_F at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. Δ_L and Δ_F among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the Δ_L and WUE_i (photosynthesis rate/transpiration rate, P_n/T_r), WUE_n (photosynthesis rate/stomatal conductance of CO_2, P_n/g_s), WUE_y (yield/crop water consumption, Y/ET_c) and yield, or between the Δ_F and WUE_y and yield were found, respectively. There were significantly negative correlations of Δ_L with WUE_i, WUE_n, WUE_y and yield (P < 0.01) at the fruit maturation stage, or Δ_L with WUE_i and WUE_n (P < 0.01) over whole growth stage, respectively. Δ_F was negatively correlated with WUE_y, WUE_n and yield (P < 0.05), but positively correlated with ET_c (P < 0.01) over the whole growth stage. Thus Δ_L or Δ_F can compare WUE_n and WUE_y, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation.     

基于MRFD的车载仪器并联减振半主动控制实验

采用3-PRRP~((4r))并联机构作为主体机构,在机构移动副处的主负荷方向上辅以磁流变阻尼器(MRFD),采用LQR最优控制算法对装置进行半主动控制,从而实现对车载仪器的多维减振.实验结果表明,该MRFD多维减振机构具有良好的多维减振效果.     

基于双目立体视觉的苗期玉米株形测

将田间正常生长的待测玉米植株带土移至测定台上,标定双目立体视觉系统,提取、分割叶片图像,以Douglas-Peucker多边形法逼近叶片边缘,去除两幅对应图像中没有匹配关系的多边形顶点,结合投影矩阵计算出叶片边缘点的三维坐标.分别投影叶片边缘点到植株平面和植株水平平面,对投影的离散点分段二次拟合、Cardinal样条插值,得到代表叶片形状的曲线,计算出叶长、叶片着生高度、茎叶夹角、叶片方位角等株形指标.测量实验表明,本方法快速、准确、自动化程度高,能够满足苗期玉米株形测量的要求.     

金属板材数控渐进成形螺旋线轨迹生成

在分析金属板材数控渐进成形特点的基础上,指出现行等高线运动成形方式存在的问题,提出了一种基于螺旋运动的数控渐进成形方式和基于STL数据模型的螺旋线成形加工轨迹的生成算法。利用顶点偏置方法生成STL模型的等距模型,采用一系列水平面和与Z轴平行的平面分别切割该等距模型,求出其交线的交点,并将其连接成螺旋线成形加工轨迹。算法应用实例表明,该算法能够生成无干涉的螺旋线成形加工轨迹。