腐霉对甲霜灵抗性测定及其生物防治

测定了腐霉属的8个种209个菌株对甲霜灵在浓度为0.5和50ug/ml时的抗性，从实验中观察到不同菌株对甲霜灵抗性变化的范围非常大，有95株在0.5ug/ml时，菌丝即停止生长，在20株在50ug/ml时，菌丝生长速度与对照几乎一样。经鉴定，这209株菌中，优势种为终极腐霉(Pythium ultimum）和刺腐霉(P.spinosum)。20株高抗菌中,P.ultimum占16株，P.irregulare占2株，P.spinosum和P.graminicola各占1株。盆栽试验表明：假单胞菌株Pseudomonas sp.CR56对高抗菌株P.ultimum ONCUR01引起黄瓜苗期猝倒病具有显著的防效，防效与甲霜灵和五氯硝基苯相比，达到极显著差异。     

Effect of extracellular HSP70/HSP70-PCs on epithelial-mesenchymal transition of human hepatocellular carcinoma HepG2 cells

AIM：To investigate the effect of extracellular heat-shock protein 70 (HSP70)/HSP70-peptide complexes (HSP70-PCs) on epithelial-mesenchymal transition (EMT) of human hepatocellular carcinoma HepG2 cells and its probable mechanism. METHODS：HepG2 cells were divided into 3 groups: control group, HSP70/HSP70-PCs (2 mg/L) group and LY294002+HSP70/HSP70-PCs group. The mRNA and protein expression of epithelial cell surface marker E-cadherin, mesenchymal cell surface marker α-smooth muscle actin (α-SMA), phosphatidylinositol 3-kinase (PI3K) and hypoxia-inducible factor 1α (HIF-1α) was examined by real-time RT-PCR and Western blotting. RESULTS：Extracellular HSP70/HSP70-PCs promoted the initiation of EMT of HepG2 cells. The expression of HIF-1α and PI3K significantly increased in the process of EMT of HepG2 cells. After PI3K was blocked by LY294002, EMT did not occur and HIF-1α was not up-regulated in HepG2 cells. CONCLUSION：Extracellular HSP70/HSP70-PCs may promote EMT of hepatocellular carcinoma cells via PI3K/HIF-1α signaling pathway.     

ND─EDS二联油乳剂灭活苗的研制

用鸡新城疫（ＮＤ）克隆３０毒株接种鸡胚，鸡产蛋下降综合症（ＥＤＳ）ＢＨ９１毒株接种鸭胚，分别收获ＮＤ鸡胚尿囊液毒和ＥＤＳ鸭胚尿囊液毒，用福尔马林灭活，二者混合后，加入１０号白油油相中，制成ＮＤ─ＥＤＳ二联油乳剂灭活苗。该苗接种在产前母鸡，可使接种鸡获得对ＮＤ和ＥＤＳ强毒攻击坚强的抵抗力。该苗安全、稳定，４℃保存期至少可达１２个月以上，适用于基层推广使用。     

通舒口服液中丹参和川芎的薄层鉴别

采用薄层层析法鉴别复方口服液中的丹参、川芎2味中药.结果表明:在薄层色谱上均能检识出丹参、川芎的色谱斑点.所用方法可行,专属性强,重复性好,可用于通舒口服液的质量控制.     

Analysis of Large Caisson Sinking Measured Resistance

In the sinking process of large-scale caisson, the distribution of the shaft friction and the end resistance is the main controlling factor of the sinking feasibility, but the research based on large-diameter piles and small or medium-scale caissons are no longer applicable. With the real-time monitoring data of the south anchorage caisson sinking process of Yangtze River Highway Bridge in Ma An Shan, this paper analyses the underlying principles and the mechanical characteristics in the sinking process of the large-scale caisson foundation, Verify the applicability of different formulas of the foundation ultimate bearing capacity in the of the caisson; Contrast sink to different depths , the value and the distribution rules of the shaft friction and the end resistance, this paper also modifies the formulas and distribution characteristics of the shaft friction proposed by current specifications, and the conclusions are suitable for reference of similar scale projects.     

Anatomical Structure of Root and Root Nodule of Alnus sibirica-Host o fBoschniakia rosica

ＡｎａｔｏｍｉｃａｌＳｔｒｕｃｔｕｒｅｏｆＲｏｏｔａｎｄＲｏｏｔＮｏｄｕｌｅｏｆＡｌｎｕｓｓｉｂｉｒｉｃａ－ＨｏｓｔｏｆＢｏｓｃｈｎｉａｋｉａｒｏｓｉｃａＨｕＢａｏｚｈｏｎｇ，ＬｉＪｉｎｇ，ＧｕｉＭｉｎｇｚｈｕ，ＬｉＧｕｉｑｉｎａｎｄＸｕＬｉｊｕｎ（...     

毕节地区林业有害生物普查

对全区林业有害生物进行普查,初步查出林业有害生物共七大类147种,病害53种,虫害83种,鼠(兔)害8种,寄生性种子植物3种,蜱螨3种,软体动物2种,有害植物1种.发现林业外来有害生物松材线虫病1种,有害植物紫茎泽兰1种,本土有害生物145种.     

除草剂对胡麻田大麦、稷（糜子）的防除效果

为筛选胡麻田大麦和稷（糜子）的高效除草剂,进行了7种除草剂防除胡麻田大麦和稷的应用研究。结果表明,108 g/L高效氟吡甲禾灵乳油、15%精吡氟禾草灵乳油、240 g/L烯草酮乳油和10%精喹禾灵乳油4种除草剂对大麦具有良好防除效果,分别在145.8、270、432 g/hm2和90 g/hm2剂量（有效成分）下药后45 d的株防效达97.39%以上,鲜重防效达99.24%以上,胡麻增产率在91.05%以上,该剂量可作为大面积示范推广的适宜剂量。50 g/L唑啉草酯乳油、108 g/L高效氟吡甲禾灵乳油、15%精吡氟禾草灵乳油、240 g/L烯草酮乳油、15%炔草酸可湿性粉剂、10%精喹禾灵乳油和12.5%烯禾啶乳油7种除草剂对稷具有良好防除效果,分别在75、145.8、270、432、135、90和412.5 g/hm2剂量下药后45 d的株防效达98.43%以上,鲜重防效达99.40%以上,胡麻增产率在84.41%以上,该剂量可作为大面积示范推广的适宜剂量。上述除草剂在推荐剂量下对胡麻安全。     

益蝽对草地贪夜蛾高龄幼虫的捕食能力评价和捕食行为观察

为了探究天敌昆虫益蝽对草地贪夜蛾的捕食潜力,本试验在室内条件下评价了益蝽5龄若虫对草地贪夜蛾6龄幼虫的捕食能力,结合田间观察总结了益蝽的捕食行为特点。结果表明：益蝽5龄若虫对草地贪夜蛾6龄幼虫的捕食功能反应符合HollingⅡ模型,对草地贪夜蛾6龄幼虫的日最大捕食量、瞬时攻击率和处理时间分别为4.671头、1.512和0.215 d;益蝽偏食活猎物,对草地贪夜蛾幼虫致死量大,当草地贪夜蛾幼虫密度大时,益蝽将一头猎物刺吸致死后并不持续吸取,而是快速攻击其他猎物,这一特点对于控制草地贪夜蛾种群数量十分有利。     

稻田恶性杂草防除药剂配方的筛选

杂草是目前严重影响水稻生产的主要障碍。经田间药效试验确定了37.5%苯.二甲水剂的适用剂量为：防治扁秆藨草、日本藨草、藨草等恶性杂草为3000-3750 mL.hm^-2;防治鸭舌草、慈菇、泽泻、谷精草和狼巴草等一般阔叶杂草则为2250 mL.hm^-2,施药适期是在杂草出齐后开花前排水喷雾茎叶处理,对水稻安全。对扁秆藨草、日本藨草和藨草的防效达98%-100%。