LY12合金冷拉棒性能的研究

对淬火前的热挤压棒用不同的冷拉拔量进行拉拔，结果表明；冷拉拔量对棒材的组织和性能有不同的影响．当变形量达到10％时，经淬火后的棒材其组织是均匀的细晶组织．     

水牛梭形住肉孢子虫抗原的薄层等电聚焦行为分析

应用薄层聚丙烯酰胺等电聚焦电泳（ＩＦＥ）对水牛梭形住肉孢子虫（Ｓａｒｃｏｃｙｓｔｉｓｆｕｓｉｆｏｒｍｉｓ）抗原进行了分析。结果表明，经ＳｅｐｈａｄｅｘＧ－１００纯化的包囊抗原在电泳图谱上显示３条相距较近的带，等电点分别为６．３０，６．４５，６．６５；缓殖子纯化抗原仅出现１条带，等电点为６．４０     

不同养殖模式翘嘴鲌肌肉营养品质比较

为评价池塘与大湖不同养殖模式翘嘴鲌(Culter alburnus)肌肉营养品质差异,试验分析了商品翘嘴鲌肌肉常规营养成分、氨基酸、脂肪酸、矿物元素含量等营养指标。结果表明:大湖翘嘴鲌肌肉水分含量显著低于池塘翘嘴鲌(P<0.05),粗蛋白质含量高于池塘翘嘴鲌,差异不显著(P>0.05)。池塘与大湖翘嘴鲌肌肉,除了赖氨酸和组氨酸以外(P<0.05),绝大部分氨基酸含量均无显著性差异(P>0.05)。大湖翘嘴鲌肌肉的总氨基酸、必需氨基酸和鲜味氨基酸含量分别较池塘翘嘴鲌高出2.72%、2.87%和2.50%。根据氨基酸评分(AAS)和化学评分(CS)结果,池塘与大湖翘嘴鲌肌肉中第一限制性氨基酸均为蛋氨酸+胱氨酸,赖氨酸相对含量最高,必需氨基酸指数(EAAI)分别为68.81和69.62。池塘翘嘴鲌的棕榈油酸(C16:1)含量显著高于大湖翘嘴鲌(P<0.05),而大湖翘嘴鲌肌肉的单不饱和脂肪酸(MUFAs)、高不饱和脂肪酸(HUFAs)、必需脂肪酸(EFA)、EPA+DHA含量则高于池塘翘嘴鲌(P>0.05)。试验检测了池塘与大湖翘嘴鲌肌肉11种矿物元素,其中镁有显著差异(P<0.05),重金属元素(铬、镉、铅)含量均在限量范围以内。综上所述,大湖养殖翘嘴鲌营养品质优于池塘养殖。     

Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree

To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (Δ_L) at different growth stages and fruit stable carbon isotope discrimination (Δ_F) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on Δ_L at different growth stages and Δ_F at fruit maturation stage. As compared with full irrigation, the average Δ_L at different growth stages and Δ_F at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. Δ_L and Δ_F among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the Δ_L and WUE_i (photosynthesis rate/transpiration rate, P_n/T_r), WUE_n (photosynthesis rate/stomatal conductance of CO_2, P_n/g_s), WUE_y (yield/crop water consumption, Y/ET_c) and yield, or between the Δ_F and WUE_y and yield were found, respectively. There were significantly negative correlations of Δ_L with WUE_i, WUE_n, WUE_y and yield (P < 0.01) at the fruit maturation stage, or Δ_L with WUE_i and WUE_n (P < 0.01) over whole growth stage, respectively. Δ_F was negatively correlated with WUE_y, WUE_n and yield (P < 0.05), but positively correlated with ET_c (P < 0.01) over the whole growth stage. Thus Δ_L or Δ_F can compare WUE_n and WUE_y, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation.     

Genetic diversity of Brassica napus L. Germplasm from China and Europe assessed by some agronomically important characters

Summary The genetic diversity and relationships among 63 rapeseed accessions, including 34 Chinese, 22 Czech, 2 Swedish, 2 German, one French and 2 Canadian accessions, were evaluated by nine agronomically important characters in the field at Yangling, Shaanxi, China. Significant differences between Chinese and European group in plant height, setting position of the first primary branch, number of siliques of the terminal raceme, thousand seed weight and seed yield per plant were detected. There were significant variations in nine agronomic characters among the tested rapeseed accessions. Ward’s minimum variance cluster analysis based on Mahalanobis distances on the raw data of nine agronomic characters clearly separated the European accessions from the Chinese ones. However, the Chinese accessions with erucic acid free and/or low glucosinolates could not be separated from those Chinese accessions with both high erucic acid and glucosinolates. In general, cluster analysis of the 63 accessions based on the selected agronomic characters was consistent with known pedigree information and geographic origin, as well as the previous RAPD results of these accessions. The European rapeseed could be important germplasm resources for enriching the genetic background of Chinese rapeseed, and vice versa.     

双波长反射法快速测定植株中的硝酸盐

为了找到一种新的快速检测植株中硝酸盐的方法,自主研制了YN-FS反射仪进行试验,通过标准溶液试验、多个植株样品的回收率实验、以及其与硝酸试粉法的对比试验,研究双波长反射法快速检测植株中硝酸盐含量的可行性。结果表明：硝酸盐含量为1.0~50.0 μg/mL时,双波长反射法快速测定植株中硝酸盐的回收率为92.8%~107.9%,测试的相对标准偏差为2.4%~4.6%,测值与标准值的绝对误差在10%以内,且其与硝酸试粉法测值呈极显著相关关系（r=0.999）。这说明双波长反射法快速检测植株中硝酸盐含量的方法是稳定可靠的,可推广使用。     

Spatial variability of soil organic carbon in a catchment of the Loess Plateau

Abstract

The issue of soil organic carbon (SOC) is of increasing concern. Because SOC, as an important soil component in farming systems, is essential for improving soil quality, sustaining food production and quality, and maintaining water quality and as a major part of the terrestrial carbon reservoir, it plays an important role in the global carbon cycle. In this paper, a total of 665 soil samples from different depths were collected randomly in the autumn of 2007, and the spatial variability of SOC content at a small catchment of the Loess Plateau was analysed using classical statistics and geo-statistical analysis. In nonsampled areas classical kriging was utilized for interpolation of SOC estimation. The classic statistical analysis revealed moderate spatial variability with all five layers of SOC-content. In addition, the average SOC content decreased with soil depth and the relationship can be modelled by an exponential equation (y=3.1795x ^?1.2015, R ²=0.9866) and all of the SOC-content data in the different depth were normally distributed. The geo-statistical analysis indicated a moderate spatial dependence in 0–60 cm, while in the 60–80 cm depth spatial dependence was strong. The semi-variogram could be fitted by an exponential model for 0–10 cm depth; by a spherical model for 10–20 cm depth and 60–80 cm depth; and by a Gaussian model for 20–60 cm depth. The range increases with increasing depth. In addition, classical kriging could successfully interpolate SOC content in the catchment. In general, the geo-statistics method on a watershed scale could be accurately used to evaluate spatial variability of the SOC content in the Loess Plateau, China.     

Sensitive determination of DNA based on the interaction between norfloxacin-Tb3+ complex and DNA

The fluorescence intensity of the norfloxacin (NFX)-Tb3+ complex enhanced by DNA was studied. Therefore, a sensitive fluorescence method for the determination of DNA was developed. The optimal conditions of the method were as follows: the hexamethylenamine (HMA)-HCl buffer was adopted for adjusting the pH to 6.5 +/- 0.1, the concentrations of NFX and Tb3+ were both fixed in 1.0 x 10(-6) mol L(-1), and the excitation and emission wavelengths were selected at 290 and 545 nm, respectively. Under the optimal conditions, the enhanced fluorescence intensity was in proportion to the concentration of DNA in the same range of 5.0 x 10(-9) - 1.0 x 10(-6) g mL(-1) for hsDNA and thermally denatured ctDNA. The detection limits (S/N = 3) were 0.9 and 0.6 ng mL(-1), respectively. In addition, the interaction between NFX-Tb3+ and DNA was discussed in detail. The experimental results from UV absorption spectra, fluorescence spectra, and the salt effect study indicated that the interaction between norfloxacin-Tb3+ complex and DNA had at least two different binding modes: the electrostatic binding and the intercalation binding. The mechanism of the fluorescence enhancement effect was also discussed.     

胡萝卜的真空微波干燥特性研究及工艺优化

利用真空微波干燥设备对胡萝卜进行正交回归干燥试验,得出各指标之间的相互影响关系及其干燥曲线,进而得到回归方程,对其进行方程的显著性检验以及参数综合优化,提出了胡萝卜真空微波干燥工艺参数的最佳组合。     

渭北旱塬核桃经济林径流林业技术应用研究

针对渭北旱塬地区干旱少雨的气候条件,布设试验样地,采取不同覆盖材料和集水整地措施,从土壤温度、含水量等方面分析其对核桃经济林造林的影响。结果表明,造林时采取一定的微型集水工程和覆盖措施,在集流节水、抑制蒸发和保蓄土壤水分等方面是有效的,薄膜、麦秸和杂草3种覆盖材料中,以薄膜覆盖集水效果最好,含水量达到21．74％,对0～20 cm土层增温效果明显,能够显著提高造林成活率,从而提高核桃经济林的产量和质量。