饲料企业成长过程中那些纠结的问题

<正>对于饲料企业的经营,除了要把握好外部环境的变化,还要关注内部环境的变化。事实上,绝大多数企业发展到一定程度之后,就会走向死亡,原因在于这些企业没有及时解决一些伴随企业成长而出现的问题。     

Structure and function of antimicrobial peptide penaeidin-5 from the black tiger shrimp Penaeus monodon

The gene for penaeidin-5, an antimicrobial peptide comprising 55 amino acids, was isolated from the hemocyte of black tiger shrimp (Penaeus monodon). RT-PCR expression tests revealed that penaeidin-5 was produced in hemocytes, gills, the intestine and muscle. Western blot analysis confirmed the panaeidin-5 was aboundantin hemocytes, the intestine and hemolymph. Immunohistochemistry revealedpenaeidin-5 in the cuticle and gills that are considered primary defense barriers. The deduced amino acid sequence of penaeidin-5 included a proline-rich N-terminal domain and a carboxyl-domain that contained six cysteine residues. Circular dichrosim analysis revealed an -helix in its secondary structure and the predicted 3D structure indicated two-disulfide bridges in the -helix. Based on the sequence of penaeidin-5 peptide cDNA, synthetic penaeidin-5 was prepared to carry out functional tests. The synthetic peptide had efficient bacteriostatic and bactericidal activity against Aerococcus viridans, and also inhibited the growth of two filamentous fungi, Fusarium pisi and Fusarium oxysporum. To measure penaeidin-5 in vivo, black tiger shrimp were challenged with Vibrio alginolyticus and A. viridans. At 3 h post-challenge, penaeidin-5 was induced and bacterial numbers decreased significantly by 12 h and 24 h.     

永磁体磁吸式排种器充种性能仿真与实验

以圆柱形永磁体为磁力排种元件设计了永磁体磁吸式精密排种器。对其充种过程进行分析,建立了充种动力学模型,并通过Matlab编程仿真分析了排种轮转速、充种角以及种子相对排种轮的摩擦因数对排种器充种率的影响,并确定了各参数的优化组合。仿真表明,充种率与排种轮转速、充种角以及种子相对排种轮的摩擦因数呈S型、指数型、对数型关系。在优化的参数组合下进行实验,其充种率可达97%。     

Role for the membrane receptor guanylyl cyclase-C in attention deficiency and hyperactive behavior

Midbrain dopamine neurons regulate many important behavioral processes, and their dysfunctions are associated with several human neuropsychiatric disorders such as attention deficit hyperactivity disorder (ADHD) and schizophrenia. Here, we report that these neurons in mice selectively express guanylyl cyclase-C (GC-C), a membrane receptor previously thought to be expressed mainly in the intestine. GC-C activation potentiates the excitatory responses mediated by glutamate and acetylcholine receptors via the activity of guanosine 3',5'-monophosphate-dependent protein kinase (PKG). Mice in which GC-C has been knocked out exhibit hyperactivity and attention deficits. Moreover, their behavioral phenotypes are reversed by ADHD therapeutics and a PKG activator. These results indicate important behavioral and physiological functions for the GC-C/PKG signaling pathway within the brain and suggest new therapeutic targets for neuropsychiatric disorders related to the malfunctions of midbrain dopamine neurons.     

花岗岩黄红壤杉木幼林施肥连年生长效应

１９９１￣１９９５年试验结果表明，在江西分宜花岗岩残积物发育的黄红壤上，杉木幼林施Ｎ、Ｐ、Ｋ及配合处理，使树高、胸径、蓄积总生长量指标分别达到５．６７￣６．５９ｍ、８．３５￣９．２８ｃｍ、６７．９２２￣９６．４１ｍ＾３／ｈｍ＾２，较对照增长５．６％￣２０．２％、７．４％￣１７．５％、２１．６％￣５６．２％。在各种施肥处理中，以５０ｋｇ／ｈｍ＾２Ｐ２Ｏ５的效果最佳，其连年生长量效应显著持续到第５年；     

榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术

选用品种(品系)、生长素(IBA、α-NAA)、质量分数3个因素,采用L16(42×29)正交设计研究榛子嫩枝扦插繁殖技术,研究生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)的活性变化.结果表明:生长素质量分数对扦插生根率影响最大,依次为品种、生长素种类.不同质量分数水平间生根率差异极显著,IBA 0.03%质量分数处理的插穗生根率最高.不同品种间生根指数差异显著,巴赛罗娜生根指数最大.榛子嫩枝扦插生根过程可分为愈伤组织形成期、诱导期和表达期3个阶段.POD活性在愈伤组织形成期呈上升趋势,表达期下降;PPO活性在愈伤组织形成期和诱导期上升,表达期下降;IAAO活性在愈伤组织形成期上升,诱导期和表达期下降.     

土壤有机质空间变异与农用地自然质量关系研究

该文以广州市花都区赤坭镇为试验区,根据典型农用地类型的土壤采样基于Cokriging方法进行土壤有机质精确插值与空间变异分析,并将结果与农用地分等成果进行相关分析。结果表明,以土壤有机质和农用地类型为协因子的球状模型Cokriging总体插值精度最高,试验区内土壤有机质分布格局是四周丘陵和水库区含量较低,巴江河两岸的平原地区、西南角的山前冲积平原含量较高,这也是灌溉水田密集的区域;试验区内土壤有机质含量菜地分布比较均匀、空间相关性很强,而水浇地分布差异较大、空间变异性较高;土壤有机质分布在南北、东北-西南两个方向相关性较强具有各向同性特征,在东南-西北、东西方向具有各向异性特征,受到随机因素的影响变异性较大。土壤有机质Cokriging插值结果与试验区内农用地自然质量等指数的相关性很显著,菜地与农用地自然质量等指数相关性最强,灌溉水田其次,旱地最差。     

转基因抗虫保铃棉(Bollgard~ひ)检测方法研究

运用常规 PCR方法和实时荧光 PCR方法对转基因抗虫保铃棉外源基因 Ca MV 35 S启动子、NOS终止子、标记基因NPT II和目的基因 Cry IA(c)进行了检测。建立了快速、准确的抗虫保铃棉籽转基因成分的 PCR检测方法。     

木材热分解动力学的研究

用ＴＧ－ＤＴＡ热分析联用技术测定了４种常用木材的ＴＧ－ＤＴＡ－５曲线，并用热动力学方法处理的ＴＧ曲线，获得了相应动力学参数。发现各种木材在干燥阶段和煅烧阶段的热性质大致相似，而炭化阶段的热性质则因木材人化学组成不同而有较大差异。