用PEG“引发”沙打旺种子活力及抗逆性的研究

应用ＰＥＧ“引发”沙打旺种子，能提高发芽率，增强发芽势，缩短发芽成苗时间。可以在北方半干旱地区充分有效地利用早春降雨。还可以提高幼苗的抗逆性，增强对早春寒的低卸能力，并探讨了ＰＥＧ渗透调控的机理和不同时间处理增强发芽势的效果。     

保持公羊精液卫生无菌的措施

公羊包皮、四肢被毛、母台羊、采精及精液处理环境条件、饲养条件、人员操作、精液稀释液等十大因素可能是造成精液污染的主要因素。因此,必须从上述各方面采取技术措施保持精液卫生无菌。     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised intercaruncular areas （IC）. In order to better understand aglandular areas called caruncular （C）, and intensely glandular the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins （DEPs） （P 〈 0.05）, functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

A miniature lighted stylet for fast oral endotracheal intubation in rabbits

Efficient oral endotracheal intubation of laboratory animals is a challenging technique in veterinary research. This study introduces a miniaturized lighted stylet for rabbit intubation. An experiment with repeated measures on two factors was used to assess the feasibility and efficacy of this method. The first factor compared stylet intubation vs. laryngoscopic intubation. The second compared three practitioners, one with prior experience and two without. Success rates on the initial attempt were not statistically different (χ² = 2.46, P = 0.12). The time difference between methods was significant (F = 41.007, P < 0.001), although the effect of practitioners was not (F = 1.038, P = 0.365). The mean ± SD of the intubation time, combining results from the three practitioners, was 20.34 ± 17.15 s for the stylet method and 57.58 ± 64.21 s for the laryngoscopic method. The results of this study demonstrate that lighted stylet intubation is efficient, robust, and independent of practitioner experience.     

日粮添加免疫生长促进剂C96对雏鸡若干血液指标的影响

选择１２０只１日龄ＡＡ商品健康雏鸡,随机分为４组,每组３０只。初步观察了日粮添加免疫生长促进剂Ｃ９６对血液中红细胞总数（ＲＢＣ）、比容（ＰＣＶ）、血红蛋白含量（Ｈｂ）、网织红细胞比例（ＲＣＣ）、白细胞总数（ＷＢＣ）等的影响。结果表明：肉雏鸡在１５ｄ时,日粮添加Ｃ９６为１１ｍｇ／ｋｇ,饲料的Ｃ９６组和疫苗－Ｃ９６组,与对照组和疫苗组比较,ＲＢＣ和Ｈｂ增高,ＷＢＣ显著下降,ＲＣＣ降低,ＰＣＶ无明显规律性变化;３０ｄ和４５ｄ时,添加Ｃ９６组和疫苗－Ｃ９６组的ＲＢＣ、Ｈｂ和ＰＣＶ,与对照组和疫苗组基本相近,ＷＢＣ和ＲＣＣ均比对照组和疫苗组低或明显低。但３０ｄ时疫苗组的ＷＢＣ比对照组高。整个试验期,ＲＢＣ、ＷＢＣ和ＰＣＶ随日龄的增长呈上升趋势,ＲＣＣ呈波动性下降。     

生长前期北京鸭维生素E需要量的研究

试验采用单因子完全随机试验设计,研究不同水平维生素E对北京鸭生产性能的影响,进而探讨0～14日龄北京鸭维生素E的需要量。试验一维生素E水平为0、5、10、20、40、100IU/kg和400IU/kg,选取392只1日龄健康雄性北京鸭随机分为7个处理组,每处理组8个重复,每重复7只。试验二采用半纯合日粮,维生素E水平为0、10、20IU/kg和100IU/kg,选取192只1日龄健康雄性北京鸭随机分为4个处理组,每处理组6个重复,每重复8只。饲养期均为14d。试验结果表明:维生素E可提高0～14日龄北京鸭生产性能,以日增重为评价指标,北京鸭维生素E最低需要量为10IU/kg。     

Dermatophagoides farinae-specific IgG responses in atopic dogs undergoing allergen-specific immunotherapy with aqueous vaccines

The molecular and immunologic mechanisms associated with successful allergen-specific immunotherapy (ASIT) have not been completely elucidated. The aim of this study was to characterize the changes in Dermatophagoides farinae -specific IgG in atopic dogs undergoing ASIT using aqueous vaccines. Fifteen atopic dogs with a positive skin test reaction to D. farinae were treated with aqueous vaccines for a minimum of 2 months following a standard protocol. Serum samples were collected before and during therapy and used to probe Western blots containing separated proteins of D. farinae . IgG responses were detected using a polyclonal goat anticanine IgG antibody and a chromogenic substrate 3,3'-diaminobenzidine. The blots were analysed using a semiquantitative digital image analysis system that evaluated the number and molecular weight of bands, as well as their intensity, which was related to IgG concentration. Prior to ASIT, all dogs showed allergen-specific IgG responses to various antigens of D. farinae . During ASIT, there was a significant increase in the total quantity of D. farinae -specific IgG antibodies to various antigens from the mite ( P  = 0.015). Significant increases were observed for a 98-kDa band ( P  = 0.015), likely to be Der f 15; bands with molecular weights between 50 and 70 kDa ( P  = 0.012); and bands between 30 and 45 kDa ( P  = 0.035). These findings provide support for the hypothesis that ASIT induces IgG blocking antibodies to allergens known to be relevant in canine atopic dermatitis.     

四种羊传染性疾病诊断试剂的测试比对试验及结果分析

随着我国改革开放的步伐加快,我国畜牧业的发展也迎来了新的大好时期。从澳大利亚和新西兰进口的种羊数量增长很快。1995年至2000年从天津口岸进口种羊约2600只,2001年至2006年约4587只。大量动物进口的同时,也带来了动物疫病传入的风险。     

保证鲜奶卫生质量的综合措施

鲜奶属于一种特殊的畜产品，难于保存和运输，如何防止鲜奶被污染，是一项非常重要的工作。现将提高鲜奶卫生质量的一些措施介绍给大家，以供参考。     

养殖场环境污染的治理

国内外治理畜禽粪便主要分为产前、产中和产后治理. 　　1 产前治理 　　发达国家对养殖场污染物的治理主要采用源头控制的对策,因为即使在对农民有巨额补贴的欧洲国家,能够采用污水处理设备的畜禽养殖场也很少,为此畜禽场的源控制,主要通过制定畜禽场农田最低配置(指畜禽场饲养量必须与周边可蓄纳畜禽粪便的农田面积相匹配)、畜禽场化粪池容量、密封性等方面的规定进行[1].在日本、欧洲大部分国家,强制要求单位面积的养殖畜禽数量,使畜禽养殖数量与地表的植物及自净能力相适应.……