Changes of immune function and mortality in patients with sepsis-induced stress hyperglycemia

AIM: To investigate the role of stress hyperglycemia on condition assessment and predicting prognosis in patients with sepsis. METHODS: The study included 44 patients with sepsis, divided into three groups according to their blood glucose profile within 24 h after admission: patients with stress hyperglycemia (group SH, n=15), diabetes mellitus type 2 (group DM, n=10), and normal glucose levels (group NG, n=19). CD4+/CD8+ ratio, Th1/Th2 ratio and HLA-DR% of the patients were measured within 24 h after admission by flow cytometry as assessment of their immune function. The sepsis-related organ failure assessment (SOFA) scores and acute physiology and chronic health evaluation II (APACHE II) scores of patients were recorded at the same time to analyze whether stress hyperglycemia affects the immune function and the 28 d mortality in patients with sepsis. RESULTS: A higher mortality rate of septic patients with stress hyperglycemia was observed compared to diabetic patients (53.3% vs 10.0%) and group NG (53.3% vs 21.1%). SOFA score and APACHE II score were higher in group SH than those in group DM and group NG while lower levels of human leucocyte antigen DR (HLA-DR) expression and CD4+/CD8+ ratio was found in group SH than those in group DM and group NG. No difference in the levels of Th1/Th2 among the three groups was observed. Non-survivors had higher levels of SOFA score, APACHEⅡ score, Th1/Th2 ratio and HLA-DR%. No difference was detected for CD4+/CD8+ ratio, mean glucose values and age. CONCLUSION: Stress hyperglycemia is associated with decreased immune function and an adverse clinical outcome in patients with sepsis.     

农业机械化组织模式及其分析

介绍和分析了国内外农业机械化的组织模式,认为组织化可以提高农业机械化服务产业的总体经济效率,也能够增加农机个体经营者的收益,同时也便于管理,减少管理成本.各地农机管理部门应重视农业机械化的组织问题,积极研究适合当地的农业机械化的组织模式,并采取适当的方法进行引导和扶持.     

FAM3C activates Akt to promote viability of oral squamous-cell carcinoma cells

AIM: To investigate the expression and roles of family with sequence similarity 3, member C (FAM3C) in oral squamous-cell carcinoma cells. METHODS: The mRNA and protein expression levels of FAM3C in dysplastic oral keratinocyte (DOK) and oral squamous-cell carcinoma WSU-HN6 cells were detected by RT-qPCR and Western blot. The WSU-HN6 cells were treated with siFAM3C or FAM3C antibody. After 24, 48 and 72 h, the viability of WSU-HN6 cells was measured by CCK-8 assay, and the activation of protein kinase B (Akt) was detected by Western blot. Adenovirus was used to mediate over-expression of FAM3C in the DOK cells. The DOK cell viability was measured by CCK-8 assay after adenovirus infection for 24, 48 and 72 h, and the activation of Akt was detected by Western blot. RESULTS: Compared with the DOK cells, the mRNA and protein levels of FAM3C were significantly increased in the WSU-HN6 cells (P<0.05). The viability of WSU-HN6 cells transfected with siFAM3C was significantly inhibited at 48 h and 72 h (P<0.05). siFAM3C treatment inhibited the activation of Akt (P<0.05). FAM3C antibody treatment also suppressed the viability of the WSU-HN6 cells at 48 h and 72 h and the activation of Akt (P<0.05). Over-expression of FAM3C in the DOK cells promoted the cell viability at 48 h and 72 h and activated Akt (P<0.05). CONCLUSION: FAM3C might promote oral squamous-cell carcinoma cell growth by activating Akt.     

黄豆芽愈伤组织培养条件对Vc积累的影响

优化了黄豆芽愈伤组织培养条件,建立了合适的黄豆芽细胞悬浮培养体系。以在25℃无激素条件下自然生长的豆芽子叶、叶芽、芽茎、芽根等部位为外植体诱导愈伤组织,以次生代谢产物Vc积累最多为目标,考察外植体、基础培养基、萘乙酸(NAA)浓度以及pH值的影响。结果表明:叶芽在pH值为5.0的B5培养基中,能有效诱导愈伤组织,且目标产物Vc积累最多。低浓度NAA对黄豆芽愈伤组织诱导及次生代谢产物Vc积累影响不大,随浓度增加,影响加大。     

不同剂量达虫净对山羊自然感染线虫的驱虫效果试验

按体重5mg/kg、10mg/kg、15mg/kg不同剂量的达虫净对自然感染线虫的山羊进行拌料饲喂驱虫,同时用0.2mg/kg阿维菌素一次皮下注射作药效对照,并设空白对照组。分别于用药前及用药后第7天取粪便进行虫卵计数。用药后第2天起每天检查由粪便中驱出的虫体数至查不出为止。结果表明:各剂量组药物对肺线虫虫卵减少率分别为100%,100%,100%,83.3%,虫卵转阴率分别为100%,100%,100%,66.7%;对捻转血矛线虫等其他线虫虫卵减少率分别为88.9%,100%,100%,100%,虫卵转阴率分别为66.7%,100%,100%,100%。用药后第2天病羊开始排虫,第3天为排虫高峰,第4天排虫减少,第5天仅见有少量虫体,第6天以后未再见虫体排出。     

合肥市高校城市森林树冠覆盖分析

以合肥市二环内、大学城和职教城3个典型区域的15所建设于不同年代的高校为研究对象,应用Arc GIS判读2018年卫星遥感影像,统计分析高校林木树冠覆盖特征。结果表明:①15所高校现实树冠覆盖面积283.25 hm~2,整体树冠覆盖率29.67%;各高校现实树冠覆盖率在11.90%～43.89%间变化,潜在树冠覆盖率在1.73%～30.00%间变化。②超过2/3的高校树冠覆盖率处在高覆盖度或极高覆盖度。③早期建设的高校现实树冠覆盖率整体上高于后期建设的,二环内高校(38.69%)最高;而在树冠覆盖潜力增长方面正好相反,职教城高校潜在树冠覆盖率(19.02%)最高。进一步分析发现,合肥市高校城市森林未来整体树冠覆盖率可提高至40.14%,不同年代区域高校树冠覆盖差异主要受时间跨度对树木的影响和高校绿化建设思路的影响。     

中国稻谷生产率的地域差异和时空分异——基于稻谷主产区的实证分析

农业结构调整的背景下,提高稻谷的生产效率对于保障我国口粮安全具有重要意义。采用1999-2015年全国籼稻和粳稻主产省的数据,利用数据包络分析法对我国各省份各年度稻谷生产效率的DEA-Malmquist指数进行了测算,通过测算得出反映稻谷全要素生产率的Malmqusit指数,并将全要素生产率分解为规模效率变化、纯技术效率变化和技术进步变化。结果表明：①稻谷主产区区域布局方面,水稻产业可以结合地区优势布局。②技术进步在稻谷的投入产出效率的提高中发挥了重要作用。在推广水稻种植中,农业部门应充分重视技术进步的作用。③发展适度规模经营,提高规模效率,提高稻谷生产效率。未来稻谷产业仍以去库存、绿色发展、规模化经营为主要趋势。     

论有机农业目标体系的整体性

本文从农业生产中人与人之间的关系、人与自然的关系,以及人与自身的关系角度,对农业效益的目标体系进行了全新的诠释,同时,建立起农业中不同利益主体目标的关联.有机农业通过政府粮食安全目标、生产者生计安全目标和消费者食品安全目标的有机整合,最大限度地实现农业经济效益、生态效益和社会效益相互间的均衡,是农业可持续发展的必然选择.     

高产优质菜用大豆品种的筛选

笔者对26个菜用大豆品种鲜荚采摘期的农艺性状及品质性状进行了研究,结果表明:科丰14、96p11、东新1号、BN-1、9118和小萤壳-1是鲜荚、鲜粒产量最优的品种;BN-1、小萤壳-1的维生素C含量最高;东新1号和诱处4号的蛋白质含量最高。以上6个菜用大豆品种适合于北京地区栽培种植。