欧盟皮内变态反应比较试验法结合ELISA检测牛结核病的研究

现在应用较多的牛结核病检疫方法有变态反应、ELISA等方法。检疫牛结核病目前OIE唯一指定方法是皮内变态反应,该方法也是最古老的方法之一,经过多次改进,仍然为各国所用。该方法的不足是非特异性反应过高,且只能检测细胞免疫状态。牛结核     

阿克苏地区鸡球虫病流行情况调查

鸡球虫病是由艾美耳属球虫引起的呈世界性分布、严重危害养鸡生产的主要鸡病之一。可造成巨大的经济损失。据统计,在各种鸡病中,球虫     

EPD估计的原理与应用

EPD是在BLUP理论的基础上对种用动物遗传值估计的一种参数。EPD值是估计育种值的一半，它是一个容易理解和应用的种畜遗传传递力的值，能很直观地反映种畜价值在其后代中的体现。EPD主要是用在同一品种中2个不同个体间直接遗传效应的比较，从而预测在同一平均遗传值水平上种用动物未来子女的性能差异。EPD适用于动物的出生、发育、母性性状、屠宰性状以及其它特征性状的估计。EPD广泛应用于肉用种公牛价值的评定。对肉牛重要经济性状EPD估计后，以出版物和（或）计算机网络的方式发布，肉牛生产者根据自己的不同需要选用种牛或精液。作者对EPD的估计原理与应用作较为详细的论述。     

Diploid female gametes induced by colchicine in Oriental lilies

The young flower buds of diploid Oriental cultivars: ‘Con. Amore’ and ‘Acapulco’ (Lilium) were treated with 0.02, 0.05, 0.1, and 0.2% colchicine to induce diploid egg (female gamete). The treated young buds between the two cultivars were crossed reciprocally as female parents with normal pollen respectively. The polyploid plants were identified by measuring stoma size and chromosome number. The results showed: that triploid progenies could be obtained through this way, and the fact that the treated young flower buds were successfully used as female parents indicated the formation of 2n or 2x egg cells. The above results implied that polyploidization by artificially induced diploid female gametes could be a powerful method to create novel variations in the breeding of Oriental lilies.     

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.     

水葫芦病原菌的研究进展

水葫芦是世界上危害最严重的水生杂草之一。目前,运用病原菌来治理水葫芦日益受到关注。本文介绍了水葫芦致病真菌、细菌和它们的有关代谢产物——植物毒素的研究进展,并探讨了利用病原菌防除水葫芦的前景。     

秆野螟属(Ostrinia)系统进化与分类研究进展

秆野螟属(Ostrinia)昆虫全球分布20种,寄主广泛,在农业生产中具有重要的经济意义。由于该属地理分布广泛,形态特征复杂,寄主和生态区重叠严重,秆野螟属系统发育研究还存在很多问题尚未解决。本文综合中国秆野螟属分类概况,对目前应用于秆野螟属系统发育研究的各种性状,包括形态特征、性信息素(生殖隔离)以及分子水平的研究进展作了阐述,并针对秆野螟属研究内容的复杂性及其研究现状,提出了相关对策。     

杂交稻对稻瘟病和稻白叶枯病的抗性鉴定

对广东省将推广的54份杂交稻组合对稻瘟病和白叶枯病的抗病性进行了鉴定。对稻瘟病的抗病性鉴定表明:抗病组合共49个,占90.7%;其中,高抗(抗性比≥91%)组合30个,占55.6%。对稻白叶枯病的抗病性鉴定表明:没有高抗(HR)和抗(R)的组合,仅有一个杂交稻组合(西胜2175)表现为中抗(mR),仅占1.9%,其余53个组合都表现感病,占98.1%。进一步分析表明:对稻瘟病表现高抗的30个杂交稻组合都不抗白叶枯病,而中抗白叶枯病的杂交稻组合西胜2175对稻瘟病表现为中抗,抗性比为74%。     

15种植物病原真菌培养滤液除草活性初步筛选

通过15种植物病原真菌的培养滤液对草坪杂草稗草、反枝苋、蒲公英、车前、苘麻进行种子萌发抑制试验,对其除草活性进行了初步研究。结果表明:玉米小斑病菌、黄瓜黑星病菌的培养滤液对稗草、蒲公英、车前都表现出显著的除草活性;玉米大斑病菌的培养滤液仅对稗草和蒲公英表现显著的除草活性;烟草赤星病菌的培养滤液仅对车前和苘麻表现了显著的除草活性。安全试验表明:烟草赤星病菌的培养滤液对黑麦草和早熟禾种子的萌发、芽和根的生长表现安全;黄瓜黑星病菌的培养滤液仅对黑麦草种子的萌发、芽和根的生长表现安全。     

土壤中花蓟马蛹的检测方法

本文对花蓟马属（Frankliniella）蛹期在土壤中存在的状态以及存在的时间进行了描述,并用直接镜检、干燥漂浮镜检、生物饲养检测等方法对土壤中蓟马蛹的检测方法进行了初步研究.生物饲养法能有效检出存在于土壤中的花蓟马蛹.