Effect of mitochondrial ATP sensitive potassium channels opening on translocation of protein kinase C epsilon in cultured adult rat ventricular myocytes

AIM: To investigate the effects of mitochondrial ATP sensitive potassium (MitoKATP) channel opening on translocation of protein kinase C epsilon (PKCε) and the relationship between the translocation of PKCε and the production of reactive oxygen species. METHODS: The expression of PKCε in cultured adult rat ventricular myocytes was investigated with immunofluorescence and Western blotting techniques. RESULTS: （1） Diazoxide, a selective MitoKATP channel opener, caused a significant translocation to myofibrillar-like structures in cultured adult rat ventricular myocytes. (2) The N-2-mercaptopropionylglycine (MPG), a free radical scavenger, partly inhibited the translocation of PKCε caused by diazoxide. (3) Chelerythrine, a selective protein kinase C (PKC) inhibitor, completely blocked the translocation of PKCε caused by diazoxide. CONCLUSION: Opening of MitoKATP channel might activate PKCε and make it translocation to myofibrillar-like structures. PKCε activation occurs downstream of MitoKATP channel, and might be caused by production of reactive oxygen species after opening of MitoKATP channel.     

Expression of telomerase inhibitor Pinx1 in leukemia cells and its correlation with telomerase activity

AIM:To study the expression of telomerase inhibitor Pinx1 in acute leukemia cells and during the differentiation of acute promyelocytic leukemia cells,and to realize its effect on telomerase activity.METHODS:Realtime quantitative PCR with fluorescence probe hybridization was used to measure the expression of Pinx1 and hTERT mRNA in acute leukemia cells and during differentiation of NB4 cells induced by ATRA.The correlations between Pinx1 and hTERT expression were also analyzed.RESULTS:Pinx1 mRNA expression in acute leukemia samples (0.00312,5.42×10-4-0.024) was significantly higher than that in normal bone marrow mononuclear cells (7.89×10-4,0-0.00863,P<0.01).The expression of Pinx1 mRNA had significant positive correlation with hTERT mRNA expression (r=0.296,P<0.05).Pinx1 mRNA expression decreased during differentiation,its expression was positive correlated with hTERT mRNA expression (r=0.900,P<0.05).CONCLUSIONS:As an inhibitor of telomerase,however,Pinx1 also had the same direction of regulation with telomerase activity in acute leukemia cells,suggesting its expression variation may be a subsequent reaction induced by that of hTERT to stabilize telomerase activity.The exact mechanisms remained to be verified.     

金叶女贞褐斑病病原鉴定

经致病性测定、病原菌形态特征观察及生物学特性测定,明确引起金叶女贞褐斑病的病原菌为素馨生棒孢(Corynespora jasminiicolaMeenu,Kharwar&;Bhartiya),并描述了其病原菌形态特征。该病菌适宜生长温度为25~30℃。用孢子悬浮液人工接种,该菌可侵染菜豆和豇豆,不侵染黄瓜、番茄、茄子和辣椒。     

国内外水果农药残留管理概况

总结和分析了我国主要水果贸易国在水果农药残留方面的标准情况概况。相对欧美、日本、加拿大等国家在水果上设立越来越苛刻的农药残留限量,我国的水果农药残留限量标准的制订和研究还比较缺乏,迫切需要根据国际食品法典要求和我国及其主要水果贸易伙伴国的水果生产和农药管理实际,制订或修订我国水果农药残留标准。     

西藏八角莲炭疽病病原鉴定及室内药剂筛选

从西藏大学农牧学院药材基地的西藏八角莲叶片病斑上分离到1种炭疽病菌,根据病害症状特点、病原菌形态特征、培养性状和致病性测定等进行鉴定,结果表明:西藏八角莲炭疽病的病原菌为半知菌亚门,腔孢纲,黑盘孢目,刺盘孢属(Colletotrichum sp.)。药剂筛选试验结果表明,对菌丝生长和孢子萌发均有强烈抑制作用的药剂有80%福.福锌可湿性粉剂、58%甲霜灵可湿性粉剂、50%多菌灵可湿性粉剂、72%霜脲.锰锌可湿性粉剂,稀释倍数在2000倍时,对菌丝和孢子的抑制率均达90%以上。     

良药美食马齿苋人工栽培技术

马齿苋，又名蚂蚁菜、长命草、酸米菜、五行草、马蛇子菜、瓜子菜，台湾通称猪母草。属于马齿苋科马齿苋属，一年生草本植物。其口味微酸，性寒滑爽，有清热解毒，健胃消积之功效，是一种常用的中草药，对预防心血管疾病和治疗热毒痢疾有很好的效果。其幼嫩茎叶可供食用。近年研究表明，无论从药理作用还是从食用保健看，马齿苋都是一种不可多得的中草药和天然绿色佳蔬。近几年来随着消费的增加，已开始转入人工栽培。     

欧盟皮内变态反应比较试验法结合ELISA检测牛结核病的研究

现在应用较多的牛结核病检疫方法有变态反应、ELISA等方法。检疫牛结核病目前OIE唯一指定方法是皮内变态反应,该方法也是最古老的方法之一,经过多次改进,仍然为各国所用。该方法的不足是非特异性反应过高,且只能检测细胞免疫状态。牛结核     

阿克苏地区鸡球虫病流行情况调查

鸡球虫病是由艾美耳属球虫引起的呈世界性分布、严重危害养鸡生产的主要鸡病之一。可造成巨大的经济损失。据统计,在各种鸡病中,球虫     

EPD估计的原理与应用

EPD是在BLUP理论的基础上对种用动物遗传值估计的一种参数。EPD值是估计育种值的一半，它是一个容易理解和应用的种畜遗传传递力的值，能很直观地反映种畜价值在其后代中的体现。EPD主要是用在同一品种中2个不同个体间直接遗传效应的比较，从而预测在同一平均遗传值水平上种用动物未来子女的性能差异。EPD适用于动物的出生、发育、母性性状、屠宰性状以及其它特征性状的估计。EPD广泛应用于肉用种公牛价值的评定。对肉牛重要经济性状EPD估计后，以出版物和（或）计算机网络的方式发布，肉牛生产者根据自己的不同需要选用种牛或精液。作者对EPD的估计原理与应用作较为详细的论述。     

Diploid female gametes induced by colchicine in Oriental lilies

The young flower buds of diploid Oriental cultivars: ‘Con. Amore’ and ‘Acapulco’ (Lilium) were treated with 0.02, 0.05, 0.1, and 0.2% colchicine to induce diploid egg (female gamete). The treated young buds between the two cultivars were crossed reciprocally as female parents with normal pollen respectively. The polyploid plants were identified by measuring stoma size and chromosome number. The results showed: that triploid progenies could be obtained through this way, and the fact that the treated young flower buds were successfully used as female parents indicated the formation of 2n or 2x egg cells. The above results implied that polyploidization by artificially induced diploid female gametes could be a powerful method to create novel variations in the breeding of Oriental lilies.