高温胁迫下水稻氨基酸态氮与铵态氮营养效应研究

在高温胁迫无菌砂培条件下对无机氮 (硫酸铵 )、有机氮 (甘氨酸 ,谷氨酸和赖氨酸 )和缺氮 (对照 )培养的水稻植株干物重、总氮量及其根、叶谷草转氨酶 (GOT)和谷丙转氨酶 (GPT)活性作了研究。结果表明 ,有机、无机氮均被水稻吸收 ,且吸收量相当。低浓度无机氮 (N 10mg/L)或有机氮培养 2 0d后的水稻植株总氮量比对照的高 50%以上 ;无机氮培养的植株干物重显著低于有机氮培养和对照 ,表明植株受NH4+毒害。培养 15d后 ,多数有机氮培养的植株根或叶GOT或GPT活性显著高于对照 ,但根、叶间有较大差别 ;而无机氮培养的植株根GOT及根、叶GPT活性是对照的 2倍或以上。高浓度无机氮 (N 500mg/L)处理 6h后稻株根、叶GPT活性分别是对照的2.5和4倍 ,而高浓度甘氨酸 ,谷氨酸和赖氨酸态氮 (N 500mg /L)处理 6h后 ,根、叶GPT活性分别是对照的 5.7、9.4、1.9倍及1.8、2.3、1.4倍。     

不同比例尺DEM提取地面坡度的精度研究——以在黄土丘陵沟壑区的试验为例

以陕北绥德县韭园沟流域为试验样区 ,采用高精度 1∶ 1万 DEM所提取的坡度为准值 ,应用多层面复合分析和比较分析的方法 ,研究该地区 1∶ 5万 DEM提取地面坡度的误差特征与纠正方法。试验结果显示 ,所获得的不同空间尺度下 DEM所提取坡度值的转换图谱 ,可对 1∶ 5万 DEM计算的地面坡度统计值进行有效修正。该成果对于 DEM数据在水土保持领域若干应用标准的制定 ,提供了重要的理论依据与技术路线     

中药渣用于培养灵芝菌及功能成分分析

该文利用中药渣培养灵芝菌,研究灵芝菌的最适发酵条件,子实体生长条件及其生物活性成分(灵芝多糖及灵芝三萜化合物)和重金属含量。结果表明:灵芝菌固体发酵最适温度为26～28℃,培养基含水量55～60％,pH值5．0～6．0,氧气充足,无需光照;子实体的适宜生长条件为:温度25～30℃,空气湿度90％左右,pH值4．0～6．0,氧气充足,需要光照。分析检测了灵芝酸多糖含量,重金属含量均在安全范围内。     

中药渣用于培养灵芝菌的研究及功能成分分析

该文利用中药渣培养灵芝菌，研究灵芝菌的最适发酵条件,子实体生长条件及其生物活性成分（灵芝多糖及灵芝三萜化合物）和重金属含量。结果表明：灵芝菌固体发酵最适温度为26～28℃，培养基含水量55～60%，pH5.0～6.0，氧气充足，无需光照；子实体的适宜生长条件为：温度25～30℃，空气湿度90%左右，pH4.0～6.0,氧气充足，需要光照。分析检测了灵芝酸多糖含量，重金属含量均在安全范围内。     

Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.     

Genotypic variation in grain cadmium concentration of lowland rice

Cadmium (Cd) contamination of paddy rice soils is commonly observed in the Yangtse River Delta, China. Large Cd uptake by rice plants and its translocation into the grains can entail human‐health risks. Genotypic variations in Cd uptake and a differential Cd partitioning into grains will be the basis for developing a rice screening or breeding tool for low grain Cd. A field experiment, conducted at the experimental farm of Jiaxing, Zhejiang province from 2002 to 2004, compared 38 rice genotypes of different types (indica vs. japonica) collected from the Yangtse River Delta. The results showed large differences in Cd concentrations in straw, brown rice, and grain chaff among the rice genotypes grown on Cd‐contaminated soil. Concentrations in brown rice ranged from 0.06 to 0.99 mg Cd kg^–1. The total Cd uptake in brown rice varied between 0.96 and 28.58 μg plant ^{– 1}. In general, indica‐type cultivars accumulated significantly more Cd than the japonica‐type cultivars. The Cd concentration in straw was highly correlated with that in brown rice. While significant differences in the Cd‐partitioning ratio (% share of total Cd uptake found in brown rice) among rice genotypes were observed, these were not correlated with Cd concentration of brown rice. This indicates that the Cd accumulation in rice grains appears to be governed mainly by the Cd uptake by the plant and probably not by differential Cd partitioning. The large genotypic variation suggests the possibility to lower the Cd content of rice by genotype selection. The development of such breeding tools should focus on low Cd uptake rather than Cd partitioning between straw and grain.     

淮河上游全新世黄土-古土壤序列元素地球化学特性研究

在野外考察研究的基础上,对河南禹州全新世风成黄土-古土壤剖面系统采样,利用X-荧光光谱仪测定了常量元素和微量元素的含量及其变化,并与磁化率曲线、粒度变化曲线对比分析发现:常量元素氧化物比值钾钠比、残积系数和以Rb为代表的微量元素含量等随剖面层次呈规律性变化,即在古土壤层(S0)中含量最高,表土层(MS)中次之,在黄土层(L1、Lt、L0)中最低。硅铝率和Sr的含量表现出相反的变化规律。表明在全新世古土壤层和表土层形成时期,气候温暖湿润,成壤作用和次生粘化作用较强;在黄土堆积时期,气候比较干旱,沙尘暴频繁,成壤作用微弱。这些化学参数与磁化率曲线可以进行对比,表明成土过程中元素的迁移变化受全新世以来气候变化和成壤环境变化的控制。与黄土高原腹地的洛川剖面相比,淮河上游禹州剖面中绝大多数常量元素的含量较高,而绝大多数微量元素的含量偏低,磁化率也显著偏低,绝对值差别很大,粒度组成当中细沙成分含量很高,这说明淮河上游黄土物质来源与黄土高原地区明显不同。联系全新世时期黄河频繁泛滥、改道、沉积与黄淮平原盛行东北风形成流沙地的事实,认为淮河上游的黄土是黄河泛滥沉积物质经过风沙活动改造,由东北风力系统搬运而来的近源粉尘堆积,再经过就地风化或者成土改造而形成。     

粒状碎屑溜砂坡树根桩固砂防护技术

 粒状碎屑溜砂坡综合加固防护技术包含控制封闭砂源区、稳定砂坡体和保护砂坡脚3部分措施。从稳定砂坡体和保护砂坡脚的一般措施入手,通过野外调查和室内模型试验,探讨花管微型树根桩固砂机制、设计要点及固砂技术。     

太湖地区氮磷肥施用对稻田氨挥发的影响

在太湖地区乌栅土上,采用田间小区试验连续两年研究了施氮(N)量为0、180、255、330kg hm-2,施磷(P2O5)量为0、309、0、180 kg hm-2的6个组合(对照N0P0、低氮N180P90、优化N255P90、低磷N255P30、高磷N255P180、高氮N330P90)以及三个施肥时期对稻田氨挥发损失的影响,氨挥发采用密闭室间歇通气法测定。结果表明,稻田氨挥发损失主要发生在施肥后6d内,基肥和第一次追肥后各处理氨挥发量占施氮量的0.4%~11.5%,而第二次追肥后氨挥发损失比例较大,对照、低氮、优化、低磷、高磷和高氮处理的氨挥发在2002年稻季分别占施氮量的5.8%、9.7%、25.6%、15.6%和11.6%,在2003年稻季则分别为27.4%、26.2%、30.0%、35.1%和27.6%。若施肥后遇阴雨天气或正值水稻拔节孕穗期,氨挥发量便降低。田面水中的NH4 -N浓度是氨挥发的决定因素之一,与氨挥发通量呈正相关。施磷量相同时,氨挥发随施氮量增加而增加;施氮量相同时,高磷和低磷处理氨挥发均高于优化处理,表明在氮磷不平衡施用时,氮肥氨挥发损失会加剧,从氨挥发损失方面考虑,稻田推荐施磷量不宜超过P2O590 kg hm-2。     

TiO2纳米粒子气固相光催化降解乙烯初探

该文对TiO2纳米粒子气固光催化降解果蔬贮藏环境乙烯技术进行了初步研究.采用溶胶-凝胶法制备的纳米TiO2薄膜作光催化剂,利用自行设计的气固光催化实验系统,研究了乙烯浓度、紫外光作用时间对光催化降解反应的影响,探讨了乙烯的光催化降解的动力学.结果显示:该研究所制备的TiO2锐钛矿型含量为48.766%,比表面积为47.186 m2/g,具有良好的光催化性能;光催化降解乙烯比直接紫外线光降解效果显著,光照10 min时光催化乙烯降解率比直接紫外线光降解提高23.76%;乙烯的降解率随着其浓度的增加而降低;乙烯的光催化降解的动力学可以用Langmuir-Hinshelwood动力学方程加以描述.