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101.
Inhibition of chicken brain neurotoxic esterase (NTE) by a series of O-halogenated-phenyl-O-alkyl phenylphosphonates was studied in vitro. The “apparent” activity was found to consist of “true” NTE (sensitive to mipafox) plus a minor mipafox-resistant component. The pI50 of O-(2,6-dichlorophenyl) O-methyl phenylphosphonate for “true” NTE was 6.65, whereas it was about 3 for mipafox-resistant hydrolysis of phenyl valerate. This compound is suitable as an alternative to mipafox in the assay of “true” NTE, whereas the use of leptophos oxon gives a less accurate measure. The ethoxy analogs are about as potent in vitro as the corresponding methoxy compounds. Leptophosoxon and ethoxyleptophosoxon are more potent in vitro inhibitors than desbromoleptophosoxon. Within a like group of chlorinated phenylphosphonates, a reasonable correlation between in vitro neurotoxic esterase inhibition of the oxon and in vivo delayed neurotoxic potential by the corresponding phosphonothionate exists. In vivo inhibition of “apparent” NTE from chicken brain, studied 24 hr after an oral dose, is dose dependent for leptophos, ethoxyleptophos, and desbromoleptophos, the latter one being a very potent in vivo inhibitor. Ethoxyleptophos and leptophos have about equal in vivo esterase inhibitory properties. For desbromoleptophos and leptophos there is good agreement between the minimum dose causing delayed neurotoxicity and the dose leading to substantial inhibition of “apparent” NTE; ethoxyleptophos, on the other hand, inhibits the esterase at a dose much lower than the one which is neurotoxic. Several possible explanations for this discrepancy are considered.  相似文献   
102.
Counter current immunoelectrophoresis (CCIE) is the diagnostic method used in the ongoing Aleutian disease virus eradication program on Danish mink farms. There has been an increasing demand for an alternative diagnostic test especially to evaluate suspected false positive CCIE reactions. We compared test results of a number of negative and positive mink sera in indirect counter current immunoelectrophoresis (ICCIE), counter current line absorption immunoelectrophoresis (CCLAIE) and radio immuno assay (RIA) with test results from counter current immunoelectrophoresis and found that counter current line absorption immunoelectrophoresis is the best alternative diagnostic screening test to counter current immunoelectrophoresis for Aleutian disease eradication programs. Not only proved the CCLAIE test to be useful for evaluation of doubtfully positive CCIE reactions, but it was found to have a higher sensitivity than the CCIE test.  相似文献   
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Function of polymorphonuclear leukocytes (PMNL) and proliferation of lymphocytes after stimulation with mitogens were evaluated in vitro at incubation temperatures of 38.5 and 42 C, and after in vivo heat stress of lactating Holstein cows. Cytochrome-c reduction and random migration of PMNL were reduced when cells were preincubated or incubated at 42 C, but high incubation temperature had little or no effect on phagocytosis and killing of Escherichia coli. Proliferation of lymphocytes was reduced when cells were incubated for 60 hours at 42 C after stimulation with phytohemagglutinin, pokeweed mitogen, or concanavalin A. After stimulation with phytohemagglutinin, lymphocytes were most sensitive to high temperature during the first 24 hours of the 60-hour culture period. High incubation temperature had little effect on viability of cells. In vivo heat stress had no significant effect on responses of PMNL in vitro, but the decrease in proliferation of lymphocytes in vitro at high temperature was less when cells were obtained from heat-stressed cows. Total leukocyte counts in blood and somatic cell counts in milk were higher in heat-stressed cows. Results indicate that: exposure to high temperature in vitro can depress responses of PMNL and lymphocytes; apparent adaptive mechanisms induced by in vivo heat stress provide protection from effects of high temperature seen in vitro; and evidence could not be found to support the hypothesis that reduction in immune function is the basis for increases in the incidence of mastitis during the summer.  相似文献   
105.
Nutritional value of quality protein maize for starter and finisher swine.   总被引:4,自引:0,他引:4  
Growth trials with starter (n = 120, 6.8 kg initially, 28 d of age, Exp. 1) and finisher (n = 70, 59 kg initially, Exp. 2) pigs were conducted to compare quality protein maize (QPM, .40% lysine) and normal corn (.31% lysine) in simple corn-based diets containing the same levels of soybean meal. In Exp. 1, pig performance was similar (P greater than .10) on all diets, regardless of the level of soybean meal, suggesting that QPM and normal corn have similar feeding value in lysine-adequate (.99 to 1.11%) diets. In Exp. 2, less soybean meal was needed in QPM than in normal corn diets to maximize performance; increasing soybean meal from 10.8 to 13.8% improved rate (P less than .05) and efficiency (P less than .01) of gain of pigs fed normal corn diets but had no effect on performance of pigs fed QPM diets. A QPM-based diet containing 6% soybean meal and supplemental lysine and tryptophan failed to maximize feed efficiency, but growth rate was equal to that obtained on the normal corn diet with 13.8% soybean meal. The apparent fecal digestibility of GE and ileal digestibility of N were similar for QPM and normal corn, but apparent ileal digestibility of most essential amino acids was slightly higher for QPM (Exp. 3). Experiment 4 compared apparent digestibilities of QPM, conventional opaque-2 corn and two high-protein corns. Digestibilities differed (P less than .05) among the corns, but the absolute differences were small and were likely due to differences in amino acid content of the corns.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   
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This study was done to characterize morphologically the spinal changes in stunted salmon, 1.5–2 years of age, spontaneously appearing in a Swedish fish farm. Radiographic examination and alizarin-alcian blue preparations showed compressed areas of the spine, most often near the dorsal fin. Of the average 50–52 vertebrae, 20–30 were often compressed, resulting in an average shortening of the fish by 23%. The histopathologic character of the shortened spine was a replacement of the chorda (intervertebral pad or disc) with a poorly differentiated hyaline cartilage, in which proliferative and degenerative processes produced a very irregular pattern. The origin of this cartilage, which had severely impaired ossifying capacity, was perichordal and it often had direct connections with the growth centers of the vertebrae. The disappearance of the large strongly convex (spheric) chorda and the arrest of the vertebral endochondral ossification resulted in narrow disc-like vertebrae in contrast to the normal X-shaped ones. The pathologic changes resemble those observed in chondrodystrophic types of perosomus ("short spine") in certain mammals, including humans, and in turkeys.  相似文献   
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ABSTRACT For more than 25 years, controversy has surrounded the characterization and differentiation of small-spored Alternaria spp. And, therefore, the application of names of several species that are involved in the pathology of diseases related to host-specific toxin production. The name A. alternata often has been broadly applied to various morphologically and chemically distinct groups of isolates from different hosts. The purpose of this study was to develop and evaluate automated and unbiased image analysis systems that will analyze different phenotypic characters and facilitate testing and application of the morphological species concept in Alternaria taxonomy. Host-specific toxin-producing Alternaria isolates assigned to five morpho-species were compared with representative isolates of morphologically distinct A. alternata. Combined results of growth rates at different temperatures, colony morphology, and metabolite profiles were found to be useful in characterization and differentiation of small-spored Alternaria spp. when standardized conditions are applied and representative isolates employed for comparison.  相似文献   
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