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91.
Steidler L Hans W Schotte L Neirynck S Obermeier F Falk W Fiers W Remaut E 《Science (New York, N.Y.)》2000,289(5483):1352-1355
The cytokine interleukin-10 (IL-10) has shown promise in clinical trials for treatment of inflammatory bowel disease (IBD). Using two mouse models, we show that the therapeutic dose of IL-10 can be reduced by localized delivery of a bacterium genetically engineered to secrete the cytokine. Intragastric administration of IL-10-secreting Lactococcus lactis caused a 50% reduction in colitis in mice treated with dextran sulfate sodium and prevented the onset of colitis in IL-10(-/-) mice. This approach may lead to better methods for cost-effective and long-term management of IBD in humans. 相似文献
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耐盐性Imt1基因表达载体的构建及其在酵母中的高效表达 总被引:2,自引:0,他引:2
将Imt1(Inositol O-methyltransferase,肌醇甲基转移酶)构建成酵母表达载体pBD#-3,在酵母双突变体gpd#+(-)(glycerol 3-phosphate-dehydrogenase,3-磷酸甘油脱氢酶)中高效表达,使gpd#+(-)突变株对氯化钠的耐性由2.5%提高到5.0%。生化分析表明,在每mg干酵母细胞内积累高达2.2nmol的Ononitol(芒柄醇)含量。Western blot分析证明了Imt1基因的表达。这一研究为植物耐盐基因工程开辟了一条新途径。 相似文献
96.
Keller HU Jorda L Küppers M Gutierrez PJ Hviid SF Knollenberg J Lara LM Sierks H Barbieri C Lamy P Rickman H Rodrigo R 《Science (New York, N.Y.)》2005,310(5746):281-283
The OSIRIS cameras (optical, spectroscopic, and infrared remote imaging system) onboard the European Space Agency's Rosetta spacecraft observed comet 9P/Tempel 1 for 17 days continuously around the time of NASA's Deep Impact mission. The cyanide-to-water production ratio was slightly enhanced in the impact cloud, compared with that of normal comet activity. Dust particles were flowing outward in the coma at >160 meters per second, accelerated by the gas. The slope of the brightness increase showed a dip about 200 seconds after the impact. Dust Afrho values before and long after the impact confirm the slight decrease of cometary activity. The dust-to-water mass ratio was much larger than 1. 相似文献
97.
Chimeras of two isoprenoid synthases catalyze all four coupling reactions in isoprenoid biosynthesis
The carbon skeletons of over 55,000 naturally occurring isoprenoid compounds are constructed from four fundamental coupling reactions: chain elongation, cyclopropanation, branching, and cyclobutanation. Enzymes that catalyze chain elongation and cyclopropanation are well studied, whereas those that catalyze branching and cyclobutanation are unknown. We have catalyzed the four reactions with chimeric proteins generated by replacing segments of a chain-elongation enzyme with corresponding sequences from a cyclopropanation enzyme. Stereochemical and mechanistic considerations suggest that the four coupling enzymes could have evolved from a common ancestor through relatively small changes in the catalytic site. 相似文献
98.
A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome 总被引:6,自引:0,他引:6
99.
Hans Jacquemyn Carmen Van Mechelen Rein Brys Olivier Honnay 《Biological conservation》2011,(1):416-422
Calcareous grasslands, most of which are man made and therefore depend on some kind of human interference for their maintenance, are among the most species-rich communities on Earth at a small scale. For many centuries, most of these grasslands have been used as extensive pasture. However, after 1900, and particularly from 1940 onwards, livestock grazing has declined throughout Europe leading to the abandonment of low intensity grasslands over large areas. To conserve the remaining grasslands or to restore recently abandoned grasslands, better insights about the effects of grassland management on above and belowground species diversity are needed. Here, we describe the results of an 11-year experiment to investigate the role of grassland management (grazing, mowing and abandonment) in determining species composition and diversity both in the aboveground vegetation and the seed bank of a calcareous grassland in Belgium. Species diversity declined by about 60% 11 years after abandonment, from 29 species m−2 to as few as 12 species m−2. Plots that were grazed remained constant in species richness, whereas mown sites lost about 20% of their original species. Abandoned plots were largely dominated by a few grass species, in particular Festuca rubra. Concomitant with changes in the aboveground vegetation, both the number of species found in the seed bank and seed density (number of seeds m−2) had changed significantly 11 years after abandonment. Species diversity and seed density were significantly lower in abandoned plots than in grazed or mown plots. We conclude that abandonment of calcareous grasslands may lead to rapid decline of plant species diversity both in the aboveground vegetation and in the seed bank. As a result, seed banks probably have a limited role to play in the restoration of recently abandoned grasslands. 相似文献
100.
Zhao Dawei Hans M. Seip Zhao Dianwu Zhang Dongbao 《Water, air, and soil pollution》1994,77(1-2):27-48
The Chongqing region located in Sichuan Province of China, comprises a large city, many small towns and rural areas. The region relies heavily on high-sulfur coal for energy production. Atmospheric pollution by sulfur oxides and other compounds from coal burning has become a major issue. Acid rain is observed throughout the region; volume weighted average pH is about 4.30. Average total S deposition for the region is estimated to be about 8 g S m?2 yr?1. In this paper we report concentrations of major ions in rain, fog, dew and clouds, of SO2 in air and of airborne particles as well as dustfall. The emissions and interactions between important chemical species in air and water droplets are discussed. 相似文献