Cigarette dust particles induced lung function injury of chronic obstructive pulmonary disease in mice

AIM: To investigate the pathogenesis of chronic obstructive disease (COPD) in mice by using nasal drip of cigarette dust particles (DSP) induced pulmonary function damage model.METHODS: BALB/c mice were randomly divided into control group, LPS 100 mg/L group, DSP 0.75 mL/L group, DSP 1.5 mL/L group and DSP 3 mL/L group for 30 days. The method of nasal drip was used for 30 days to establish the COPD model. Rrs, Ers, Crs, Est, Cst, P-3/8Rn, P-3/8G and P-3/8H were measured for evaluating lung function of the mice in each group by the method of FlexiVent. The effect on the increase of airway resistance induced by methacholine (Mach) was determined using main bronchial rings by Myograph method. The HE, Masson and Resorcinol fuchsin staining of mouse tracheas and lung tissues were conducted. RESULTS: Continuous nasal drip with DSP for 30 days increased Rrs, Ers, Est, P-3/8Rn and decreased Crs, Cst, P-3/8G and P-3/8H in the mice. DSP significantly shifted the dose-effect curve of tracheal contraction induced by Mach to the left, increased the sensitivity of the airway to Mach, and significantly increased the maximal contractile airway effect of Mach. Exposure to DSP caused fibrosis of airway subepithelial, deposition of collagen in the airway basement membrane under the reticular plate, induced reticular plate thickening, pulmonary bronchial lumen serious deformation, and the inflammatory cell infiltration in the lung of mice. Significantly increased alveolar wall muscle fibers and collagen fibers were also observed. CONCLUSION: The lung function and pathomorphological changes of COPD mice induced by 30 days nasal drip of cigarette dust particles were similar to those of human COPD.     

紫丁香叶中丁香苦苷和橄榄苦苷的质量分数

分别采用甲醇回流法和水煮法对5—10月份采集的12组紫丁香树叶样品进行提取,制备提取物,并通过HPLC分析其中丁香苦苷和橄榄苦苷的质量分数。结果表明,醇提法优于水提法,紫丁香树叶中同时含有大量的丁香苦苷和橄榄苦苷,并且二者变化规律相同,分别在5月下旬、6月下旬和8—9月份出现3个高峰期。在5月20日、6月20日和9月5日采集的树叶中,干燥树叶丁香苦苷的质量分数分别为:8.28%、3.77%和3.82%;橄榄苦苷的质量分数分别为:3.75%、0.90%和1.16%。     

类胡萝卜素的功能研究进展(综述)

β-胡萝卜素和类胡萝卡素广泛存在自然界中。作为维生素A的前体,可提高人体的免疫能力,也是一种抗氧化剂,可淬灭与清除机体内产生的自由基。重要的是β-胡萝卜素和类胡萝卜素能预防癌症和减缓癌症的发展,细胞缝间联结的理论支持了类胡萝卜素的具有这一效果的看法,但是根据人群调查也出现类胡萝卜素具有负结果的报道。本文就类胡萝卜素的营养功能的研究现状作一概要综述。     

盆栽大丽花的花期控制研究

探讨了影响大丽花开花的若干因素。在试验中利用扦插不定芽来繁殖种苗,采取盆栽独本大丽花的方式,分别于5年里的3月至10月间对63个大丽花品种的花期控制进行了研究。将大丽花按开花先后分为早花、中花、晚花三个品种类群;并运用全息生物学基本原理对取自根颈不同部位插穗所繁殖时后代的开花情况进行了分析;确定了营养水平会给大丽花花期早晚带来重要影响。从而总结出控制大丽花开花的具体措施,使其成品出圃率从50%左右提高到80%左右,显著增加了盆栽大丽花的观赏、商品价值。     

高校横向科研项目的发展形势及建议

通过研究高校横向科研项目目前的发展形势及横向科研项目的特点,提出横向科研项目发展的建议,以供参考。     

日粮添加硒碘铜对宁夏地区奶牛生产和繁殖性能的影响

选择宁夏地区８５８头奶牛进行日粮添加硒、碘、铜试验,其中对照组２６７头,其余分别添加硒、碘,硒、铜,或硒、碘、铜。于添加前后４次采样,测定血液和肝脏的硒、碘、铜含量。结果表明：添加硒和碘的奶牛血硒、肝脏和血清蛋白结合碘极显著地高于对照组（Ｐ＜０．０１）,白肌病发病率由１７．９％至０;死胎、弱犊发生率由２２．９７％降至６．７９％;硒、碘、铜组奶牛妊娠率,平均情期受胎率和一次情期受胎率分别上升１０．４     

雷公藤总DNA提取方法的研究

由于雷公藤叶片富含多糖及酚类物质,要获得高质量的DNA有一定难度。本研究以雷公藤植物叶片为材料,对传统提取方法进行改进、优化,比较了4种不同的提取方法,最终获得了一种以CTAB法为基础,试剂盒纯化的获得高质量DNA的方法。试验结果表明,使用该方法从雷公藤叶片中分离的DNA,纯度高、杂质少,且相对于其他方法,试验成功率明显提高,适用性强。     

人工栽培雷公藤水浸提液对杉木种子发芽的化感效应

利用水浸提的方式对人工栽培雷公藤的生化物质进行提取,通过杉木种子发芽实验进行生物检测.分析结果表明：人工栽培雷公藤的水浸提物在高浓度下能够抑制杉木种子的萌发和生长,但是在中、低浓度下对杉木种子的萌发和生长有显著的促进作用,这有可能为雷公藤与杉木合理混交密度的研究提供可靠的实验依据.     

4个野生早熟禾属草种的耐热性研究

以草地早熟禾栽培种"午夜"为对照,通过测定高温胁迫下4个野生早熟禾属草种的几项生理指标,探讨了半致死温度、叶片细胞质膜透性、叶片脯氨酸含量以及叶片丙二醛含量等生理指标的变化特征.结果表明草地早熟禾、硬质早熟禾、细叶早熟禾、早熟禾和"午夜"的半致死温度分别为56.56℃、55.95℃、53.78℃、52.88℃和56.09℃;高温胁迫下,各草种随着胁迫程度和胁迫时间的增加,都表现为质膜透性增大,游离脯氨酸积累先增加后减少,丙二醛含量持续增长.4种草受高温胁迫时伤害的程度存在差异,耐热能力强弱依次为草地早熟禾、硬质早熟禾、早熟禾、细叶早熟禾.     

Effects of soil moisture content on CO2 triggered soil physicochemical properties in a near-surface environment

Journal of Soils and Sediments - Carbon capture and storage (CCS) has been frequently discussed as a strategy for meeting CO2 emission reduction and its targets. However, some critical issues have...