二化螟dsRNA降解酶基因的克隆与表达分析

为解析二化螟Chilo suppressalis体内参与降解双链RNA(double-stranded RNA,dsRNA)的关键核酸酶的功能,克隆二化螟不同的非专一性核酸酶（non-specific nuclease,NUC）基因,并对这些基因进行生物信息学分析和组织定量表达分析,同时对dsRNA降解酶（dsRNA degrading nuclease,dsRNase）活力的组织分布进行研究。结果显示,共克隆获得5个NUC基因,其中有4个编码dsRNase亚家族基因（CsdsRNase1～CsdsRNase4）和1个编码Endonuclease G亚家族基因（CsEndoG）。5个NUC基因的开放阅读框核苷酸序列长度范围为828～1 338 bp,编码275～445个氨基酸残基,其分子量大小为31.68～49.57 kD,预测等电点为5.48～9.42。CsdsRNase1和CsdsRNase2含有信号肽序列,两者相似度极高,且与家蚕Bombyx mori和斜纹夜蛾Spodoptera litura中具有dsRNA降解酶活力的dsRNase同源聚类;CsdsRNase1和CsdsRN...     

生态技术在现代农业中的地位及其技术群构成

提出了现代农业的主要支撑技术包括生态技术、生物技术和管理技术,并分析了此三类支撑技术的相互关系;通过深入比较,指出生态技术在现代农业中应占主导地位,这是现代农业发展的内在要求,是中国生态环境现状的必然选择,是建立社会主义和谐社会的必然趋势;同时提出了生态技术群中应该重点发展的主要技术:立体农业技术、综合养殖生态技术、特色农业技术、多色农业技术、设施农业技术、农业综合节水技术、农业污染综合防治技术和无公害绿色农产品标准化生产技术等。     

多胺对甜瓜幼苗抗冷性的影响机理研究

本文研究了外源多胺对在低温胁迫下甜瓜幼苗体内DNA、RNA、可溶性蛋白质、超氧物歧化酶(SOD)及细胞质膜透性的影响.结果表明,多胺处理的甜瓜幼苗在低温(2±1℃)下处理10h后,RNA和DNA含量明显增多,蛋白质的图谱发生显著变化,SOD活性增强,细胞膜的破损渗漏较少,尤以0.4mmol的腐胺处理最为显著.多胺与核酸的相互作用调节了植物体内的代谢过程,与膜的相互作用增强了机体的抗冷性.     

Design of Preparation of High Grade WC Powder from Voilet WO2.72

Synthesis of W powder by oxide reduction method and fabrication of WC powder by carbonization of W powder are investigated, using violet and blue tungsten oxides as the raw materials, respectively, for the preparation of excellent high grade WC powder. Microstructure and properties of the two raw materials and the obtained W powders and WC powders are studied. The results show that violet tungsten is a suitable raw material for synthesizing uhrafine W powder using the conventional method. The W and WC powders produced from the violet tungsten oxide have better physical properties than those produced from blue tungsten oxide.     

Decision Making for Vendor Selection With AHP and Random Disposal of DEA

The vendor selection directly relatives to the procurement quality and influences the competition of the corporation in the supply chains. It is important and practical to study the vendor selection method. The measuring criteria and the method for vendor selection are analyzed. The authors put forward an new method which is combined with both the advantage of analytic hierarchy process (AHP ) and random disposal of data envelopment analysis ( DEA) for vendor selection. It introduces a random variable which solves the disadvantage of the weigh value in DEA which convert subjective estimation to objective opinion in the course of the vendor selecting. The method can improve the reliability of the vendor selection.     

Review on Synthesis Refrectory Nanoparticles

In general, the process of synthesis the refractory nanoparticles Is classified as solid state, liquid state and gaseity. The new progress and development in the study of refractory nanoparticles are investigated. The synthesis technology of the refrectory nanoparticle in liquid state is discussed mostly. The refrectory nanoparticles is synthesized by the liquid state. The characteristics and the structure of the refrectory nanoparticles have been studied in this paper.     

玉米自交系及杂交种抗青枯病鉴定

1986-1989年采用自然鉴定和人工土壤接种鉴定的方法，对602份玉米材料的青枯病抗性进行了鉴定。人工接种鉴定397份，其中204份材料表现抗病。在正常年份，人工接种鉴定效果明显好于自然鉴定，但在重病区或青枯病流行年份，在尚未具备人工接种条件时，利用自然发病作为选育抗病材料仍有一定效果。玉米苗期接种青枯病重于成株期     

基于网络的模具CAD／CAE／CAM一体化系统的研究

对基于网络的模具CAD／CAE／CAM一体化系统进行了研究，提出了基于ASP技术的系统体系结构和框架模型。对系统开发过程中的数据交换技术、数据库开发和管理技术、系统核心组件的开发和集成技术进行了探讨。给出了该系统在轿车行李箱后盖拉 模设计和制造中的应用实例。     

基于模式搜索的轮胎纵向刚度估计方法

提出了一种含有两个参数的轮胎纵向力模型,并通过该模型利用基于模式搜索的最小二乘法对轮胎的纵向刚度和滚动半径进行了动态估计。仿真证明了该算法的可行性。将该算法应用于车辆的实际试验数据,结果证明轮胎的压力与轮胎纵向刚度近似成反比。